Molecular tools for understanding population structure in Antarctic species
During the last decade, methods for detecting DNA polymorphisms have proliferated at a bewildering pace. Today the investigator must choose among various types of genetic markers as well as between a variety of methods for discovering and screening polymorphisms. Polymorphisms useful for the analysi...
Published in: | Antarctic Science |
---|---|
Main Author: | |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Cambridge University Press (CUP)
2000
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1017/s0954102000000353 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102000000353 |
id |
crcambridgeupr:10.1017/s0954102000000353 |
---|---|
record_format |
openpolar |
spelling |
crcambridgeupr:10.1017/s0954102000000353 2024-03-03T08:38:41+00:00 Molecular tools for understanding population structure in Antarctic species Gaffney, Patrick M. 2000 http://dx.doi.org/10.1017/s0954102000000353 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102000000353 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Antarctic Science volume 12, issue 3, page 288-296 ISSN 0954-1020 1365-2079 Geology Ecology, Evolution, Behavior and Systematics Oceanography journal-article 2000 crcambridgeupr https://doi.org/10.1017/s0954102000000353 2024-02-08T08:43:45Z During the last decade, methods for detecting DNA polymorphisms have proliferated at a bewildering pace. Today the investigator must choose among various types of genetic markers as well as between a variety of methods for discovering and screening polymorphisms. Polymorphisms useful for the analysis of population structure are found in both mitochondrial and nuclear genomes. Marker development may proceed along two routes: 1) discovery of species–specific markers, and 2) application of universal methods. Species-specific markers are based on sequence data from the target species or close relatives, whereas universal markers are based on the use of polymerase chain reaction (PCR) primers targeted to regions highly conserved across diverse taxa. Markers commonly employed include mitochondrial DNA polymorphisms, microsatellites, anonymous nuclear loci and known genes (both coding and noncoding regions). Methods for detecting polymorphisms range from technically simple (RFLP analysis) to more sophisticated mutation scanning methods. We review the application of these approaches to several key Antarctic species (the Patagonian toothfish Dissostichus eleginoides , the mackerel icefish Champsocephalus gunnari , and the squid Martialia hyadesi Rochebrune & Mabille, 1889) and present preliminary data on genetic polymorphisms in toothfish and icefish. Article in Journal/Newspaper Antarc* Antarctic Antarctic Science Icefish Patagonian Toothfish Cambridge University Press Antarctic Antarctic Science 12 3 288 296 |
institution |
Open Polar |
collection |
Cambridge University Press |
op_collection_id |
crcambridgeupr |
language |
English |
topic |
Geology Ecology, Evolution, Behavior and Systematics Oceanography |
spellingShingle |
Geology Ecology, Evolution, Behavior and Systematics Oceanography Gaffney, Patrick M. Molecular tools for understanding population structure in Antarctic species |
topic_facet |
Geology Ecology, Evolution, Behavior and Systematics Oceanography |
description |
During the last decade, methods for detecting DNA polymorphisms have proliferated at a bewildering pace. Today the investigator must choose among various types of genetic markers as well as between a variety of methods for discovering and screening polymorphisms. Polymorphisms useful for the analysis of population structure are found in both mitochondrial and nuclear genomes. Marker development may proceed along two routes: 1) discovery of species–specific markers, and 2) application of universal methods. Species-specific markers are based on sequence data from the target species or close relatives, whereas universal markers are based on the use of polymerase chain reaction (PCR) primers targeted to regions highly conserved across diverse taxa. Markers commonly employed include mitochondrial DNA polymorphisms, microsatellites, anonymous nuclear loci and known genes (both coding and noncoding regions). Methods for detecting polymorphisms range from technically simple (RFLP analysis) to more sophisticated mutation scanning methods. We review the application of these approaches to several key Antarctic species (the Patagonian toothfish Dissostichus eleginoides , the mackerel icefish Champsocephalus gunnari , and the squid Martialia hyadesi Rochebrune & Mabille, 1889) and present preliminary data on genetic polymorphisms in toothfish and icefish. |
format |
Article in Journal/Newspaper |
author |
Gaffney, Patrick M. |
author_facet |
Gaffney, Patrick M. |
author_sort |
Gaffney, Patrick M. |
title |
Molecular tools for understanding population structure in Antarctic species |
title_short |
Molecular tools for understanding population structure in Antarctic species |
title_full |
Molecular tools for understanding population structure in Antarctic species |
title_fullStr |
Molecular tools for understanding population structure in Antarctic species |
title_full_unstemmed |
Molecular tools for understanding population structure in Antarctic species |
title_sort |
molecular tools for understanding population structure in antarctic species |
publisher |
Cambridge University Press (CUP) |
publishDate |
2000 |
url |
http://dx.doi.org/10.1017/s0954102000000353 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102000000353 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctic Science Icefish Patagonian Toothfish |
genre_facet |
Antarc* Antarctic Antarctic Science Icefish Patagonian Toothfish |
op_source |
Antarctic Science volume 12, issue 3, page 288-296 ISSN 0954-1020 1365-2079 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0954102000000353 |
container_title |
Antarctic Science |
container_volume |
12 |
container_issue |
3 |
container_start_page |
288 |
op_container_end_page |
296 |
_version_ |
1792507107570876416 |