Genetic detection of Dobrava-Belgrade hantavirus in the edible dormouse ( Glis glis) in central Serbia
SUMMARY Hantaviruses are endemic in the Balkans, particularly in Serbia, where sporadic cases and/or outbreaks of hantaviral human disease have been reported repeatedly, and evidenced serologically. Here, we present genetic detection of Dobrava-Belgrade virus (DOBV) hantaviral sequences in wild rode...
Published in: | Epidemiology and Infection |
---|---|
Main Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Cambridge University Press (CUP)
2014
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1017/s0950268814001010 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0950268814001010 |
id |
crcambridgeupr:10.1017/s0950268814001010 |
---|---|
record_format |
openpolar |
spelling |
crcambridgeupr:10.1017/s0950268814001010 2024-09-15T18:18:46+00:00 Genetic detection of Dobrava-Belgrade hantavirus in the edible dormouse ( Glis glis) in central Serbia STANOJEVIC, M. NIKOLIC, V. STAJKOVIC, N. STAMENKOVIC, G. BOZOVIC, B. CEKANAC, R. MARUSIC, P. GLIGIC, A. 2014 http://dx.doi.org/10.1017/s0950268814001010 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0950268814001010 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Epidemiology and Infection volume 143, issue 2, page 400-404 ISSN 0950-2688 1469-4409 journal-article 2014 crcambridgeupr https://doi.org/10.1017/s0950268814001010 2024-08-07T04:04:09Z SUMMARY Hantaviruses are endemic in the Balkans, particularly in Serbia, where sporadic cases and/or outbreaks of hantaviral human disease have been reported repeatedly, and evidenced serologically. Here, we present genetic detection of Dobrava-Belgrade virus (DOBV) hantaviral sequences in wild rodents trapped in central Serbia. All the animals were pre-screened serologically by indirect immunofluorescence (IF) test and only those with a positive finding of hantaviral antigens were further tested by polymerase chain reaction. Of the total of 104 trapped animals, 20 were found to be IF positive and of those three were positive for hantaviral RNA: one Microtus arvalis for Tula virus, and one each of Apodemus agrarius and Glis glis for DOBV. Phylogenetic analysis of the obtained sequences implies putative DOBV spillover infection of A. agrarius and G. glis from Apodemus flavicollis. However, future investigations should help to identify the most common natural host and geographical distribution of DOBV in its reservoir hosts in Serbia. Article in Journal/Newspaper Microtus arvalis Cambridge University Press Epidemiology and Infection 143 2 400 404 |
institution |
Open Polar |
collection |
Cambridge University Press |
op_collection_id |
crcambridgeupr |
language |
English |
description |
SUMMARY Hantaviruses are endemic in the Balkans, particularly in Serbia, where sporadic cases and/or outbreaks of hantaviral human disease have been reported repeatedly, and evidenced serologically. Here, we present genetic detection of Dobrava-Belgrade virus (DOBV) hantaviral sequences in wild rodents trapped in central Serbia. All the animals were pre-screened serologically by indirect immunofluorescence (IF) test and only those with a positive finding of hantaviral antigens were further tested by polymerase chain reaction. Of the total of 104 trapped animals, 20 were found to be IF positive and of those three were positive for hantaviral RNA: one Microtus arvalis for Tula virus, and one each of Apodemus agrarius and Glis glis for DOBV. Phylogenetic analysis of the obtained sequences implies putative DOBV spillover infection of A. agrarius and G. glis from Apodemus flavicollis. However, future investigations should help to identify the most common natural host and geographical distribution of DOBV in its reservoir hosts in Serbia. |
format |
Article in Journal/Newspaper |
author |
STANOJEVIC, M. NIKOLIC, V. STAJKOVIC, N. STAMENKOVIC, G. BOZOVIC, B. CEKANAC, R. MARUSIC, P. GLIGIC, A. |
spellingShingle |
STANOJEVIC, M. NIKOLIC, V. STAJKOVIC, N. STAMENKOVIC, G. BOZOVIC, B. CEKANAC, R. MARUSIC, P. GLIGIC, A. Genetic detection of Dobrava-Belgrade hantavirus in the edible dormouse ( Glis glis) in central Serbia |
author_facet |
STANOJEVIC, M. NIKOLIC, V. STAJKOVIC, N. STAMENKOVIC, G. BOZOVIC, B. CEKANAC, R. MARUSIC, P. GLIGIC, A. |
author_sort |
STANOJEVIC, M. |
title |
Genetic detection of Dobrava-Belgrade hantavirus in the edible dormouse ( Glis glis) in central Serbia |
title_short |
Genetic detection of Dobrava-Belgrade hantavirus in the edible dormouse ( Glis glis) in central Serbia |
title_full |
Genetic detection of Dobrava-Belgrade hantavirus in the edible dormouse ( Glis glis) in central Serbia |
title_fullStr |
Genetic detection of Dobrava-Belgrade hantavirus in the edible dormouse ( Glis glis) in central Serbia |
title_full_unstemmed |
Genetic detection of Dobrava-Belgrade hantavirus in the edible dormouse ( Glis glis) in central Serbia |
title_sort |
genetic detection of dobrava-belgrade hantavirus in the edible dormouse ( glis glis) in central serbia |
publisher |
Cambridge University Press (CUP) |
publishDate |
2014 |
url |
http://dx.doi.org/10.1017/s0950268814001010 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0950268814001010 |
genre |
Microtus arvalis |
genre_facet |
Microtus arvalis |
op_source |
Epidemiology and Infection volume 143, issue 2, page 400-404 ISSN 0950-2688 1469-4409 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0950268814001010 |
container_title |
Epidemiology and Infection |
container_volume |
143 |
container_issue |
2 |
container_start_page |
400 |
op_container_end_page |
404 |
_version_ |
1810456842349838336 |