Ultrastructural Changes in the Root Meristem Cells of Rubus Chamaemorus L. (Cloudberry)
In the course of studies in the root meristem tissue of Rubus chamaemorus L. some important changes in the ultrastructural morphology were observed during the initiation of senescence at the end of the growing season. Root meristems were collected from naturally growing healthy populations of Cloudb...
Published in: | Proceedings, annual meeting, Electron Microscopy Society of America |
---|---|
Main Author: | |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Cambridge University Press (CUP)
1975
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1017/s0424820100116437 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0424820100116437 |
id |
crcambridgeupr:10.1017/s0424820100116437 |
---|---|
record_format |
openpolar |
spelling |
crcambridgeupr:10.1017/s0424820100116437 2024-03-03T08:48:30+00:00 Ultrastructural Changes in the Root Meristem Cells of Rubus Chamaemorus L. (Cloudberry) Bal, Arya K. 1975 http://dx.doi.org/10.1017/s0424820100116437 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0424820100116437 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Proceedings, annual meeting, Electron Microscopy Society of America volume 33, page 562-563 ISSN 0424-8201 2690-1315 General Medicine journal-article 1975 crcambridgeupr https://doi.org/10.1017/s0424820100116437 2024-02-08T08:44:27Z In the course of studies in the root meristem tissue of Rubus chamaemorus L. some important changes in the ultrastructural morphology were observed during the initiation of senescence at the end of the growing season. Root meristems were collected from naturally growing healthy populations of Cloudberry plants, and fixed in Karnovsky's mixture or in 2.5% glutaraldehyde in phosphate buffer. The samples were osmicated, dehydrated following usual methods and embedded in Epon. Ultrathin sections were stained in uranyl acetate and lead citrate. Figure 1 shows part of a dense cell in the meristem. The electron density of these cells is due to large amounts of a particulate material in the cytoplasmic matrix. The smallest particle seen in electron micrographs is about 40 A, although larger aggregates are also found, which remain randomly distributed in association with various cell organelles. Dense substance has been found associated with golgi membranes, proplastids, vacuoles and microtubules (Fig. 2). Article in Journal/Newspaper Rubus chamaemorus Cambridge University Press Proceedings, annual meeting, Electron Microscopy Society of America 33 562 563 |
institution |
Open Polar |
collection |
Cambridge University Press |
op_collection_id |
crcambridgeupr |
language |
English |
topic |
General Medicine |
spellingShingle |
General Medicine Bal, Arya K. Ultrastructural Changes in the Root Meristem Cells of Rubus Chamaemorus L. (Cloudberry) |
topic_facet |
General Medicine |
description |
In the course of studies in the root meristem tissue of Rubus chamaemorus L. some important changes in the ultrastructural morphology were observed during the initiation of senescence at the end of the growing season. Root meristems were collected from naturally growing healthy populations of Cloudberry plants, and fixed in Karnovsky's mixture or in 2.5% glutaraldehyde in phosphate buffer. The samples were osmicated, dehydrated following usual methods and embedded in Epon. Ultrathin sections were stained in uranyl acetate and lead citrate. Figure 1 shows part of a dense cell in the meristem. The electron density of these cells is due to large amounts of a particulate material in the cytoplasmic matrix. The smallest particle seen in electron micrographs is about 40 A, although larger aggregates are also found, which remain randomly distributed in association with various cell organelles. Dense substance has been found associated with golgi membranes, proplastids, vacuoles and microtubules (Fig. 2). |
format |
Article in Journal/Newspaper |
author |
Bal, Arya K. |
author_facet |
Bal, Arya K. |
author_sort |
Bal, Arya K. |
title |
Ultrastructural Changes in the Root Meristem Cells of Rubus Chamaemorus L. (Cloudberry) |
title_short |
Ultrastructural Changes in the Root Meristem Cells of Rubus Chamaemorus L. (Cloudberry) |
title_full |
Ultrastructural Changes in the Root Meristem Cells of Rubus Chamaemorus L. (Cloudberry) |
title_fullStr |
Ultrastructural Changes in the Root Meristem Cells of Rubus Chamaemorus L. (Cloudberry) |
title_full_unstemmed |
Ultrastructural Changes in the Root Meristem Cells of Rubus Chamaemorus L. (Cloudberry) |
title_sort |
ultrastructural changes in the root meristem cells of rubus chamaemorus l. (cloudberry) |
publisher |
Cambridge University Press (CUP) |
publishDate |
1975 |
url |
http://dx.doi.org/10.1017/s0424820100116437 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0424820100116437 |
genre |
Rubus chamaemorus |
genre_facet |
Rubus chamaemorus |
op_source |
Proceedings, annual meeting, Electron Microscopy Society of America volume 33, page 562-563 ISSN 0424-8201 2690-1315 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0424820100116437 |
container_title |
Proceedings, annual meeting, Electron Microscopy Society of America |
container_volume |
33 |
container_start_page |
562 |
op_container_end_page |
563 |
_version_ |
1792505379775578112 |