Ultrastructural Changes in the Root Meristem Cells of Rubus Chamaemorus L. (Cloudberry)

• Published in: Proceedings, annual meeting, Electron Microscopy Society of America
• Main Author: Bal, Arya K.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Cambridge University Press (CUP) 1975
• Subjects: General Medicine; Rubus chamaemorus
• Online Access: http://dx.doi.org/10.1017/s0424820100116437; https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0424820100116437

In the course of studies in the root meristem tissue of Rubus chamaemorus L. some important changes in the ultrastructural morphology were observed during the initiation of senescence at the end of the growing season. Root meristems were collected from naturally growing healthy populations of Cloudberry plants, and fixed in Karnovsky's mixture or in 2.5% glutaraldehyde in phosphate buffer. The samples were osmicated, dehydrated following usual methods and embedded in Epon. Ultrathin sections were stained in uranyl acetate and lead citrate. Figure 1 shows part of a dense cell in the meristem. The electron density of these cells is due to large amounts of a particulate material in the cytoplasmic matrix. The smallest particle seen in electron micrographs is about 40 A, although larger aggregates are also found, which remain randomly distributed in association with various cell organelles. Dense substance has been found associated with golgi membranes, proplastids, vacuoles and microtubules (Fig. 2).