Ultrastructural studies of an aquatic bacterium, seliberia stellata
Seliberia stellata is a bacterium first isolated from tundra soil and described as a spirally twisted rod having one subpolar flagellum and reproducing by fission or budding. The cells stick at their poles forming a rosette or star form. Although this procaryotic organism has been noted in aquatic e...
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Cambridge University Press (CUP)
1978
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Online Access: | http://dx.doi.org/10.1017/s0424820100082832 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0424820100082832 |
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crcambridgeupr:10.1017/s0424820100082832 2024-03-03T08:49:14+00:00 Ultrastructural studies of an aquatic bacterium, seliberia stellata Swafford, J. R. Schmidt, J. M. 1978 http://dx.doi.org/10.1017/s0424820100082832 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0424820100082832 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Proceedings, annual meeting, Electron Microscopy Society of America volume 36, issue 2, page 394-395 ISSN 0424-8201 2690-1315 General Medicine journal-article 1978 crcambridgeupr https://doi.org/10.1017/s0424820100082832 2024-02-08T08:33:32Z Seliberia stellata is a bacterium first isolated from tundra soil and described as a spirally twisted rod having one subpolar flagellum and reproducing by fission or budding. The cells stick at their poles forming a rosette or star form. Although this procaryotic organism has been noted in aquatic environments such as fresh water ponds and laboratory distilled water, strains from these habitats have apparently not been obtained in pure culture. We have isolated the bacteria from two fresh water sources and maintained them as pure cultures in dilute peptone-yeast extract broth or 1% agar media. Isolation of two strains of these organisms was achieved by enriching water samples with 0. 005% peptone. Enrichment culture surfaces were evaluated for the presence of bacteria by placing carbon-stabilized formvar grids onto the unbroken surface film followed by immediate removal and staining with 0. 25 or 0. 5% potassium phosphotungstate for TEM. The occurrence of Seliberia-like cells in these enrichments was rare (approximately 1/1000). Article in Journal/Newspaper Tundra Cambridge University Press Proceedings, annual meeting, Electron Microscopy Society of America 36 2 394 395 |
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Cambridge University Press |
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English |
topic |
General Medicine |
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General Medicine Swafford, J. R. Schmidt, J. M. Ultrastructural studies of an aquatic bacterium, seliberia stellata |
topic_facet |
General Medicine |
description |
Seliberia stellata is a bacterium first isolated from tundra soil and described as a spirally twisted rod having one subpolar flagellum and reproducing by fission or budding. The cells stick at their poles forming a rosette or star form. Although this procaryotic organism has been noted in aquatic environments such as fresh water ponds and laboratory distilled water, strains from these habitats have apparently not been obtained in pure culture. We have isolated the bacteria from two fresh water sources and maintained them as pure cultures in dilute peptone-yeast extract broth or 1% agar media. Isolation of two strains of these organisms was achieved by enriching water samples with 0. 005% peptone. Enrichment culture surfaces were evaluated for the presence of bacteria by placing carbon-stabilized formvar grids onto the unbroken surface film followed by immediate removal and staining with 0. 25 or 0. 5% potassium phosphotungstate for TEM. The occurrence of Seliberia-like cells in these enrichments was rare (approximately 1/1000). |
format |
Article in Journal/Newspaper |
author |
Swafford, J. R. Schmidt, J. M. |
author_facet |
Swafford, J. R. Schmidt, J. M. |
author_sort |
Swafford, J. R. |
title |
Ultrastructural studies of an aquatic bacterium, seliberia stellata |
title_short |
Ultrastructural studies of an aquatic bacterium, seliberia stellata |
title_full |
Ultrastructural studies of an aquatic bacterium, seliberia stellata |
title_fullStr |
Ultrastructural studies of an aquatic bacterium, seliberia stellata |
title_full_unstemmed |
Ultrastructural studies of an aquatic bacterium, seliberia stellata |
title_sort |
ultrastructural studies of an aquatic bacterium, seliberia stellata |
publisher |
Cambridge University Press (CUP) |
publishDate |
1978 |
url |
http://dx.doi.org/10.1017/s0424820100082832 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0424820100082832 |
genre |
Tundra |
genre_facet |
Tundra |
op_source |
Proceedings, annual meeting, Electron Microscopy Society of America volume 36, issue 2, page 394-395 ISSN 0424-8201 2690-1315 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0424820100082832 |
container_title |
Proceedings, annual meeting, Electron Microscopy Society of America |
container_volume |
36 |
container_issue |
2 |
container_start_page |
394 |
op_container_end_page |
395 |
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1792506410751229952 |