Surface Ultrastructure of Gram (−) Bacteria: II. Determination of the Gram Reaction in Antartic Soil Bacteria by Electron Microscopy
A modified negative staining technique (“OTPT”) has been described in the study of the surface topography of bacteria. This technique is useful in the rapid and accurate identification and discrimination of the Gram reaction of unknown bacteria under circumstances unfavorable to the direct applicati...
Published in: | Proceedings, annual meeting, Electron Microscopy Society of America |
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Cambridge University Press (CUP)
1974
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Online Access: | http://dx.doi.org/10.1017/s0424820100050901 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0424820100050901 |
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crcambridgeupr:10.1017/s0424820100050901 2024-03-03T08:38:04+00:00 Surface Ultrastructure of Gram (−) Bacteria: II. Determination of the Gram Reaction in Antartic Soil Bacteria by Electron Microscopy Uydess, I. L. Vishniac, Wolf 1974 http://dx.doi.org/10.1017/s0424820100050901 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0424820100050901 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Proceedings, annual meeting, Electron Microscopy Society of America volume 32, page 178-179 ISSN 0424-8201 2690-1315 General Medicine journal-article 1974 crcambridgeupr https://doi.org/10.1017/s0424820100050901 2024-02-08T08:41:50Z A modified negative staining technique (“OTPT”) has been described in the study of the surface topography of bacteria. This technique is useful in the rapid and accurate identification and discrimination of the Gram reaction of unknown bacteria under circumstances unfavorable to the direct application of the Gram stain. Cells of a particular genus exhibit the same basic topographical features independent of cell age, culture conditions or other cellular parameters. Several pure cultures of unclassified bacteria isolated from Antarctic soils were prepared by the OTPT staining method. Although initial attempts employing the Gram stain were often inconclusive, this technique allowed a positive identification of all samples. Two organisms were chosen for more detailed study. Article in Journal/Newspaper Antarc* Antarctic antartic* Cambridge University Press Antarctic Proceedings, annual meeting, Electron Microscopy Society of America 32 178 179 |
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Cambridge University Press |
op_collection_id |
crcambridgeupr |
language |
English |
topic |
General Medicine |
spellingShingle |
General Medicine Uydess, I. L. Vishniac, Wolf Surface Ultrastructure of Gram (−) Bacteria: II. Determination of the Gram Reaction in Antartic Soil Bacteria by Electron Microscopy |
topic_facet |
General Medicine |
description |
A modified negative staining technique (“OTPT”) has been described in the study of the surface topography of bacteria. This technique is useful in the rapid and accurate identification and discrimination of the Gram reaction of unknown bacteria under circumstances unfavorable to the direct application of the Gram stain. Cells of a particular genus exhibit the same basic topographical features independent of cell age, culture conditions or other cellular parameters. Several pure cultures of unclassified bacteria isolated from Antarctic soils were prepared by the OTPT staining method. Although initial attempts employing the Gram stain were often inconclusive, this technique allowed a positive identification of all samples. Two organisms were chosen for more detailed study. |
format |
Article in Journal/Newspaper |
author |
Uydess, I. L. Vishniac, Wolf |
author_facet |
Uydess, I. L. Vishniac, Wolf |
author_sort |
Uydess, I. L. |
title |
Surface Ultrastructure of Gram (−) Bacteria: II. Determination of the Gram Reaction in Antartic Soil Bacteria by Electron Microscopy |
title_short |
Surface Ultrastructure of Gram (−) Bacteria: II. Determination of the Gram Reaction in Antartic Soil Bacteria by Electron Microscopy |
title_full |
Surface Ultrastructure of Gram (−) Bacteria: II. Determination of the Gram Reaction in Antartic Soil Bacteria by Electron Microscopy |
title_fullStr |
Surface Ultrastructure of Gram (−) Bacteria: II. Determination of the Gram Reaction in Antartic Soil Bacteria by Electron Microscopy |
title_full_unstemmed |
Surface Ultrastructure of Gram (−) Bacteria: II. Determination of the Gram Reaction in Antartic Soil Bacteria by Electron Microscopy |
title_sort |
surface ultrastructure of gram (−) bacteria: ii. determination of the gram reaction in antartic soil bacteria by electron microscopy |
publisher |
Cambridge University Press (CUP) |
publishDate |
1974 |
url |
http://dx.doi.org/10.1017/s0424820100050901 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0424820100050901 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic antartic* |
genre_facet |
Antarc* Antarctic antartic* |
op_source |
Proceedings, annual meeting, Electron Microscopy Society of America volume 32, page 178-179 ISSN 0424-8201 2690-1315 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0424820100050901 |
container_title |
Proceedings, annual meeting, Electron Microscopy Society of America |
container_volume |
32 |
container_start_page |
178 |
op_container_end_page |
179 |
_version_ |
1792504263756218368 |