Know your enemy – transcriptome of myxozoan Tetracapsuloides bryosalmonae reveals potential drug targets against proliferative kidney disease in salmonids
Abstract The myxozoan Tetracapsuloides bryosalmonae is a widely spread endoparasite that causes proliferative kidney disease (PKD) in salmonid fish. We developed an in silico pipeline to separate transcripts of T. bryosalmonae from the kidney tissue of its natural vertebrate host, brown trout ( Salm...
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Cambridge University Press (CUP)
2021
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Online Access: | http://dx.doi.org/10.1017/s003118202100010x https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S003118202100010X |
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crcambridgeupr:10.1017/s003118202100010x 2024-06-23T07:51:24+00:00 Know your enemy – transcriptome of myxozoan Tetracapsuloides bryosalmonae reveals potential drug targets against proliferative kidney disease in salmonids Ahmad, Freed Debes, Paul V. Pukk, Lilian Kahar, Siim Hartikainen, Hanna Gross, Riho Vasemägi, Anti 2021 http://dx.doi.org/10.1017/s003118202100010x https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S003118202100010X en eng Cambridge University Press (CUP) http://creativecommons.org/licenses/by/4.0/ Parasitology volume 148, issue 6, page 726-739 ISSN 0031-1820 1469-8161 journal-article 2021 crcambridgeupr https://doi.org/10.1017/s003118202100010x 2024-06-12T04:04:07Z Abstract The myxozoan Tetracapsuloides bryosalmonae is a widely spread endoparasite that causes proliferative kidney disease (PKD) in salmonid fish. We developed an in silico pipeline to separate transcripts of T. bryosalmonae from the kidney tissue of its natural vertebrate host, brown trout ( Salmo trutta ). After stringent filtering, we constructed a partial transcriptome assembly T. bryosalmonae , comprising 3427 transcripts. Based on homology-restricted searches of the assembled parasite transcriptome and Atlantic salmon ( Salmo salar ) proteome, we identified four protein targets (Endoglycoceramidase, Legumain-like protease, Carbonic anhydrase 2, Pancreatic lipase-related protein 2) for the development of anti-parasitic drugs against T. bryosalmonae . Earlier work of these proteins on parasitic protists and helminths suggests that the identified anti-parasitic drug targets represent promising chemotherapeutic candidates also against T. bryosalmonae , and strengthen the view that the known inhibitors can be effective in evolutionarily distant organisms. In addition, we identified differentially expressed T. bryosalmonae genes between moderately and severely infected fish, indicating an increased abundance of T. bryosalmonae sporogonic stages in fish with low parasite load. In conclusion, this study paves the way for future genomic research in T. bryosalmonae and represents an important step towards the development of effective drugs against PKD. Article in Journal/Newspaper Atlantic salmon Salmo salar Cambridge University Press Parasitology 148 6 726 739 |
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Cambridge University Press |
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English |
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Abstract The myxozoan Tetracapsuloides bryosalmonae is a widely spread endoparasite that causes proliferative kidney disease (PKD) in salmonid fish. We developed an in silico pipeline to separate transcripts of T. bryosalmonae from the kidney tissue of its natural vertebrate host, brown trout ( Salmo trutta ). After stringent filtering, we constructed a partial transcriptome assembly T. bryosalmonae , comprising 3427 transcripts. Based on homology-restricted searches of the assembled parasite transcriptome and Atlantic salmon ( Salmo salar ) proteome, we identified four protein targets (Endoglycoceramidase, Legumain-like protease, Carbonic anhydrase 2, Pancreatic lipase-related protein 2) for the development of anti-parasitic drugs against T. bryosalmonae . Earlier work of these proteins on parasitic protists and helminths suggests that the identified anti-parasitic drug targets represent promising chemotherapeutic candidates also against T. bryosalmonae , and strengthen the view that the known inhibitors can be effective in evolutionarily distant organisms. In addition, we identified differentially expressed T. bryosalmonae genes between moderately and severely infected fish, indicating an increased abundance of T. bryosalmonae sporogonic stages in fish with low parasite load. In conclusion, this study paves the way for future genomic research in T. bryosalmonae and represents an important step towards the development of effective drugs against PKD. |
format |
Article in Journal/Newspaper |
author |
Ahmad, Freed Debes, Paul V. Pukk, Lilian Kahar, Siim Hartikainen, Hanna Gross, Riho Vasemägi, Anti |
spellingShingle |
Ahmad, Freed Debes, Paul V. Pukk, Lilian Kahar, Siim Hartikainen, Hanna Gross, Riho Vasemägi, Anti Know your enemy – transcriptome of myxozoan Tetracapsuloides bryosalmonae reveals potential drug targets against proliferative kidney disease in salmonids |
author_facet |
Ahmad, Freed Debes, Paul V. Pukk, Lilian Kahar, Siim Hartikainen, Hanna Gross, Riho Vasemägi, Anti |
author_sort |
Ahmad, Freed |
title |
Know your enemy – transcriptome of myxozoan Tetracapsuloides bryosalmonae reveals potential drug targets against proliferative kidney disease in salmonids |
title_short |
Know your enemy – transcriptome of myxozoan Tetracapsuloides bryosalmonae reveals potential drug targets against proliferative kidney disease in salmonids |
title_full |
Know your enemy – transcriptome of myxozoan Tetracapsuloides bryosalmonae reveals potential drug targets against proliferative kidney disease in salmonids |
title_fullStr |
Know your enemy – transcriptome of myxozoan Tetracapsuloides bryosalmonae reveals potential drug targets against proliferative kidney disease in salmonids |
title_full_unstemmed |
Know your enemy – transcriptome of myxozoan Tetracapsuloides bryosalmonae reveals potential drug targets against proliferative kidney disease in salmonids |
title_sort |
know your enemy – transcriptome of myxozoan tetracapsuloides bryosalmonae reveals potential drug targets against proliferative kidney disease in salmonids |
publisher |
Cambridge University Press (CUP) |
publishDate |
2021 |
url |
http://dx.doi.org/10.1017/s003118202100010x https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S003118202100010X |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Parasitology volume 148, issue 6, page 726-739 ISSN 0031-1820 1469-8161 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1017/s003118202100010x |
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148 |
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1802642485084684288 |