The mitochondrial genome of Angiostrongylus mackerrasae is distinct from A. cantonensis and A. malaysiensis
Abstract The native rat lungworm ( Angiostrongylus mackerrasae ) and the invasive rat lungworm ( Angiostrongylus cantonensis ) occur in eastern Australia. The species identity of A. mackerrasae remained unquestioned until relatively recently, when compilation of mtDNA data indicated that A. mackerra...
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crcambridgeupr:10.1017/s0031182020000232 2024-06-23T07:56:24+00:00 The mitochondrial genome of Angiostrongylus mackerrasae is distinct from A. cantonensis and A. malaysiensis Valentyne, Hayley Spratt, David M. Aghazadeh, Mahdis Jones, Malcolm K. Šlapeta, Jan 2020 http://dx.doi.org/10.1017/s0031182020000232 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0031182020000232 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Parasitology volume 147, issue 6, page 681-688 ISSN 0031-1820 1469-8161 journal-article 2020 crcambridgeupr https://doi.org/10.1017/s0031182020000232 2024-06-05T04:03:45Z Abstract The native rat lungworm ( Angiostrongylus mackerrasae ) and the invasive rat lungworm ( Angiostrongylus cantonensis ) occur in eastern Australia. The species identity of A. mackerrasae remained unquestioned until relatively recently, when compilation of mtDNA data indicated that A. mackerrasae sensu Aghazadeh et al . (2015 b ) clusters within A. cantonensis based on their mitochondrial genomes (mtDNA). To re-evaluate the species identity of A. mackerrasae , we sought material that would be morphologically conspecific with A. mackerrasae . We combined morphological and molecular approaches to confirm or refute the specific status of A. mackerrasae . Nematodes conspecific with A. mackerrasae from Rattus fuscipes and Rattus rattus were collected in Queensland, Australia. Morphologically identified A. mackerrasae voucher specimens were characterized using amplification of cox1 followed by the generation of reference complete mtDNA. The morphologically distinct A. cantonensis , A. mackerrasae and A. malaysiensis are genetically distinguishable forming a monophyletic mtDNA lineage. We conclude that A. mackerrasae sensu Aghazadeh et al . (2015 b ) is a misidentified specimen of A. cantonensis . The availability of the mtDNA genome of A. mackerrasae enables its unequivocal genetic identification and differentiation from other Angiostrongylus species. Article in Journal/Newspaper Rattus rattus Cambridge University Press Queensland Parasitology 147 6 681 688 |
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Cambridge University Press |
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English |
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Abstract The native rat lungworm ( Angiostrongylus mackerrasae ) and the invasive rat lungworm ( Angiostrongylus cantonensis ) occur in eastern Australia. The species identity of A. mackerrasae remained unquestioned until relatively recently, when compilation of mtDNA data indicated that A. mackerrasae sensu Aghazadeh et al . (2015 b ) clusters within A. cantonensis based on their mitochondrial genomes (mtDNA). To re-evaluate the species identity of A. mackerrasae , we sought material that would be morphologically conspecific with A. mackerrasae . We combined morphological and molecular approaches to confirm or refute the specific status of A. mackerrasae . Nematodes conspecific with A. mackerrasae from Rattus fuscipes and Rattus rattus were collected in Queensland, Australia. Morphologically identified A. mackerrasae voucher specimens were characterized using amplification of cox1 followed by the generation of reference complete mtDNA. The morphologically distinct A. cantonensis , A. mackerrasae and A. malaysiensis are genetically distinguishable forming a monophyletic mtDNA lineage. We conclude that A. mackerrasae sensu Aghazadeh et al . (2015 b ) is a misidentified specimen of A. cantonensis . The availability of the mtDNA genome of A. mackerrasae enables its unequivocal genetic identification and differentiation from other Angiostrongylus species. |
format |
Article in Journal/Newspaper |
author |
Valentyne, Hayley Spratt, David M. Aghazadeh, Mahdis Jones, Malcolm K. Šlapeta, Jan |
spellingShingle |
Valentyne, Hayley Spratt, David M. Aghazadeh, Mahdis Jones, Malcolm K. Šlapeta, Jan The mitochondrial genome of Angiostrongylus mackerrasae is distinct from A. cantonensis and A. malaysiensis |
author_facet |
Valentyne, Hayley Spratt, David M. Aghazadeh, Mahdis Jones, Malcolm K. Šlapeta, Jan |
author_sort |
Valentyne, Hayley |
title |
The mitochondrial genome of Angiostrongylus mackerrasae is distinct from A. cantonensis and A. malaysiensis |
title_short |
The mitochondrial genome of Angiostrongylus mackerrasae is distinct from A. cantonensis and A. malaysiensis |
title_full |
The mitochondrial genome of Angiostrongylus mackerrasae is distinct from A. cantonensis and A. malaysiensis |
title_fullStr |
The mitochondrial genome of Angiostrongylus mackerrasae is distinct from A. cantonensis and A. malaysiensis |
title_full_unstemmed |
The mitochondrial genome of Angiostrongylus mackerrasae is distinct from A. cantonensis and A. malaysiensis |
title_sort |
mitochondrial genome of angiostrongylus mackerrasae is distinct from a. cantonensis and a. malaysiensis |
publisher |
Cambridge University Press (CUP) |
publishDate |
2020 |
url |
http://dx.doi.org/10.1017/s0031182020000232 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0031182020000232 |
geographic |
Queensland |
geographic_facet |
Queensland |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_source |
Parasitology volume 147, issue 6, page 681-688 ISSN 0031-1820 1469-8161 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0031182020000232 |
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Parasitology |
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147 |
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6 |
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681 |
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688 |
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1802649456889298944 |