DNA barcoding of ancient parasites
SUMMARY Ancient samples present a number of technical challenges for DNA barcoding, including damaged DNA with low endogenous copy number and short fragment lengths. Nevertheless, techniques are available to overcome these issues, and DNA barcoding has now been used to successfully recover parasite...
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Online Access: | http://dx.doi.org/10.1017/s0031182018000380 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0031182018000380 |
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crcambridgeupr:10.1017/s0031182018000380 2024-09-15T18:30:04+00:00 DNA barcoding of ancient parasites WOOD, JAMIE R. 2018 http://dx.doi.org/10.1017/s0031182018000380 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0031182018000380 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Parasitology volume 145, issue 5, page 646-655 ISSN 0031-1820 1469-8161 journal-article 2018 crcambridgeupr https://doi.org/10.1017/s0031182018000380 2024-08-28T04:00:57Z SUMMARY Ancient samples present a number of technical challenges for DNA barcoding, including damaged DNA with low endogenous copy number and short fragment lengths. Nevertheless, techniques are available to overcome these issues, and DNA barcoding has now been used to successfully recover parasite DNA from a wide variety of ancient substrates, including coprolites, cesspit sediment, mummified tissues, burial sediments and permafrost soils. The study of parasite DNA from ancient samples can provide a number of unique scientific insights, for example: (1) into the parasite communities and health of prehistoric human populations; (2) the ability to reconstruct the natural parasite faunas of rare or extinct host species, which has implications for conservation management and de-extinction; and (3) the ability to view in ‘real-time’ processes that may operate over century- or millenial-timescales, such as how parasites responded to past climate change events or how they co-evolved alongside their hosts. The application of DNA metabarcoding and high-throughput sequencing to ancient specimens has so far been limited, but in future promises great potential for gaining empirical data on poorly understood processes such as parasite co-extinction. Article in Journal/Newspaper permafrost Cambridge University Press Parasitology 145 5 646 655 |
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Cambridge University Press |
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crcambridgeupr |
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English |
description |
SUMMARY Ancient samples present a number of technical challenges for DNA barcoding, including damaged DNA with low endogenous copy number and short fragment lengths. Nevertheless, techniques are available to overcome these issues, and DNA barcoding has now been used to successfully recover parasite DNA from a wide variety of ancient substrates, including coprolites, cesspit sediment, mummified tissues, burial sediments and permafrost soils. The study of parasite DNA from ancient samples can provide a number of unique scientific insights, for example: (1) into the parasite communities and health of prehistoric human populations; (2) the ability to reconstruct the natural parasite faunas of rare or extinct host species, which has implications for conservation management and de-extinction; and (3) the ability to view in ‘real-time’ processes that may operate over century- or millenial-timescales, such as how parasites responded to past climate change events or how they co-evolved alongside their hosts. The application of DNA metabarcoding and high-throughput sequencing to ancient specimens has so far been limited, but in future promises great potential for gaining empirical data on poorly understood processes such as parasite co-extinction. |
format |
Article in Journal/Newspaper |
author |
WOOD, JAMIE R. |
spellingShingle |
WOOD, JAMIE R. DNA barcoding of ancient parasites |
author_facet |
WOOD, JAMIE R. |
author_sort |
WOOD, JAMIE R. |
title |
DNA barcoding of ancient parasites |
title_short |
DNA barcoding of ancient parasites |
title_full |
DNA barcoding of ancient parasites |
title_fullStr |
DNA barcoding of ancient parasites |
title_full_unstemmed |
DNA barcoding of ancient parasites |
title_sort |
dna barcoding of ancient parasites |
publisher |
Cambridge University Press (CUP) |
publishDate |
2018 |
url |
http://dx.doi.org/10.1017/s0031182018000380 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0031182018000380 |
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permafrost |
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permafrost |
op_source |
Parasitology volume 145, issue 5, page 646-655 ISSN 0031-1820 1469-8161 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0031182018000380 |
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Parasitology |
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145 |
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5 |
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646 |
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655 |
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1810471548606218240 |