The role of the mussel Mytilus spp. in the transmission of ostreid herpesvirus-1 microVar
Abstract The Pacific oyster Crassostrea gigas contributes significantly to global aquaculture; however, C. gigas culture has been affected by ostreid herpesvirus-1 (OsHV-1) and variants. The dynamics of how the virus maintains itself at culture sites is unclear and the role of carriers, reservoirs o...
Published in: | Parasitology |
---|---|
Main Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Cambridge University Press (CUP)
2017
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1017/s0031182017002244 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0031182017002244 |
id |
crcambridgeupr:10.1017/s0031182017002244 |
---|---|
record_format |
openpolar |
spelling |
crcambridgeupr:10.1017/s0031182017002244 2024-09-15T18:03:16+00:00 The role of the mussel Mytilus spp. in the transmission of ostreid herpesvirus-1 microVar O’ Reilly, A. J. Laide, C. Maloy, A Hutton, S. Bookelaar, B. O’ Sullivan, K. Lynch, S. A. Culloty, S. C. 2017 http://dx.doi.org/10.1017/s0031182017002244 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0031182017002244 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Parasitology volume 145, issue 8, page 1095-1104 ISSN 0031-1820 1469-8161 journal-article 2017 crcambridgeupr https://doi.org/10.1017/s0031182017002244 2024-07-31T04:04:19Z Abstract The Pacific oyster Crassostrea gigas contributes significantly to global aquaculture; however, C. gigas culture has been affected by ostreid herpesvirus-1 (OsHV-1) and variants. The dynamics of how the virus maintains itself at culture sites is unclear and the role of carriers, reservoirs or hosts is unknown. Both wild and cultured mussels Mytilus spp. ( Mytilus edulis , Mytilus galloprovincialis and hybrids) are commonly found at C. gigas culture sites. The objective of this study was to investigate if Mytilus spp. can harbour the virus and if viral transmission can occur between mussels and oysters. Mytilus spp. living at oyster trestles, 400–500 m higher up the shore from the trestles and up to 26 km at non-culture sites were screened for OsHV-1 and variants by all the World Organization for Animal Health (OIE) recommended diagnostic methods including polymerase chain reaction (PCR), quantitative PCR (qPCR), histology, in situ hybridization and confirmation using direct sequencing. The particular primers that target OsHV-1 and variants, including OsHV-1 microVar ( μ Var), were used in the PCR and qPCR. OsHV-1 μ Var was detected in wild Mytilus spp. at C. gigas culture sites and more significantly the virus was detected in mussels at non-culture sites. Cohabitation of exposed wild mussels and naïve C. gigas resulted in viral transmission after 14 days, under an elevated temperature regime. These results indicate that mussels can harbour OsHV-1 μ Var; however, the impact of OsHV-1 μ Var on Mytilus spp. requires further investigation. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Cambridge University Press Parasitology 145 8 1095 1104 |
institution |
Open Polar |
collection |
Cambridge University Press |
op_collection_id |
crcambridgeupr |
language |
English |
description |
Abstract The Pacific oyster Crassostrea gigas contributes significantly to global aquaculture; however, C. gigas culture has been affected by ostreid herpesvirus-1 (OsHV-1) and variants. The dynamics of how the virus maintains itself at culture sites is unclear and the role of carriers, reservoirs or hosts is unknown. Both wild and cultured mussels Mytilus spp. ( Mytilus edulis , Mytilus galloprovincialis and hybrids) are commonly found at C. gigas culture sites. The objective of this study was to investigate if Mytilus spp. can harbour the virus and if viral transmission can occur between mussels and oysters. Mytilus spp. living at oyster trestles, 400–500 m higher up the shore from the trestles and up to 26 km at non-culture sites were screened for OsHV-1 and variants by all the World Organization for Animal Health (OIE) recommended diagnostic methods including polymerase chain reaction (PCR), quantitative PCR (qPCR), histology, in situ hybridization and confirmation using direct sequencing. The particular primers that target OsHV-1 and variants, including OsHV-1 microVar ( μ Var), were used in the PCR and qPCR. OsHV-1 μ Var was detected in wild Mytilus spp. at C. gigas culture sites and more significantly the virus was detected in mussels at non-culture sites. Cohabitation of exposed wild mussels and naïve C. gigas resulted in viral transmission after 14 days, under an elevated temperature regime. These results indicate that mussels can harbour OsHV-1 μ Var; however, the impact of OsHV-1 μ Var on Mytilus spp. requires further investigation. |
format |
Article in Journal/Newspaper |
author |
O’ Reilly, A. J. Laide, C. Maloy, A Hutton, S. Bookelaar, B. O’ Sullivan, K. Lynch, S. A. Culloty, S. C. |
spellingShingle |
O’ Reilly, A. J. Laide, C. Maloy, A Hutton, S. Bookelaar, B. O’ Sullivan, K. Lynch, S. A. Culloty, S. C. The role of the mussel Mytilus spp. in the transmission of ostreid herpesvirus-1 microVar |
author_facet |
O’ Reilly, A. J. Laide, C. Maloy, A Hutton, S. Bookelaar, B. O’ Sullivan, K. Lynch, S. A. Culloty, S. C. |
author_sort |
O’ Reilly, A. J. |
title |
The role of the mussel Mytilus spp. in the transmission of ostreid herpesvirus-1 microVar |
title_short |
The role of the mussel Mytilus spp. in the transmission of ostreid herpesvirus-1 microVar |
title_full |
The role of the mussel Mytilus spp. in the transmission of ostreid herpesvirus-1 microVar |
title_fullStr |
The role of the mussel Mytilus spp. in the transmission of ostreid herpesvirus-1 microVar |
title_full_unstemmed |
The role of the mussel Mytilus spp. in the transmission of ostreid herpesvirus-1 microVar |
title_sort |
role of the mussel mytilus spp. in the transmission of ostreid herpesvirus-1 microvar |
publisher |
Cambridge University Press (CUP) |
publishDate |
2017 |
url |
http://dx.doi.org/10.1017/s0031182017002244 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0031182017002244 |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Parasitology volume 145, issue 8, page 1095-1104 ISSN 0031-1820 1469-8161 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0031182017002244 |
container_title |
Parasitology |
container_volume |
145 |
container_issue |
8 |
container_start_page |
1095 |
op_container_end_page |
1104 |
_version_ |
1810440783033008128 |