Cryptosporidiuminfecting wild cricetid rodents from the subfamilies Arvicolinae and Neotominae
SUMMARY We undertook a study on Cryptosporidium spp. in wild cricetid rodents. Fecal samples were collected from meadow voles ( Microtus pennsylvanicus ), southern red-backed voles ( Myodes gapperi ), woodland voles ( Microtus pinetorum ), muskrats ( Ondatra zibethicus ) and Peromyscus spp. mice in...
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Online Access: | http://dx.doi.org/10.1017/s0031182017001524 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0031182017001524 |
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crcambridgeupr:10.1017/s0031182017001524 2024-09-15T18:18:46+00:00 Cryptosporidiuminfecting wild cricetid rodents from the subfamilies Arvicolinae and Neotominae STENGER, BRIANNA L. S. HORČIČKOVÁ, MICHAELA CLARK, MARK E. KVÁČ, MARTIN ČONDLOVÁ, ŠÁRKA KHAN, EAKALAK WIDMER, GIOVANNI XIAO, LIHUA GIDDINGS, CATHERINE W. PENNIL, CHRISTOPHER STANKO, MICHAL SAK, BOHUMIL MCEVOY, JOHN M. 2017 http://dx.doi.org/10.1017/s0031182017001524 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0031182017001524 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Parasitology volume 145, issue 3, page 326-334 ISSN 0031-1820 1469-8161 journal-article 2017 crcambridgeupr https://doi.org/10.1017/s0031182017001524 2024-07-24T04:03:15Z SUMMARY We undertook a study on Cryptosporidium spp. in wild cricetid rodents. Fecal samples were collected from meadow voles ( Microtus pennsylvanicus ), southern red-backed voles ( Myodes gapperi ), woodland voles ( Microtus pinetorum ), muskrats ( Ondatra zibethicus ) and Peromyscus spp. mice in North America, and from bank voles ( Myodes glareolus ) and common voles ( Microtus arvalis ) in Europe. Isolates were characterized by sequence and phylogenetic analyses of the small subunit ribosomal RNA (SSU) and actin genes. Overall, 33·2% (362/1089) of cricetids tested positive for Cryptosporidium , with a greater prevalence in cricetids from North America (50·7%; 302/596) than Europe (12·1%; 60/493). Principal Coordinate analysis separated SSU sequences into three major groups (G1-G3), each represented by sequences from North American and European cricetids. A maximum likelihood tree of SSU sequences had low bootstrap support and showed G1 to be more heterogeneous than G2 or G3. Actin and concatenated actin-SSU trees, which were better resolved and had higher bootstrap support than the SSU phylogeny, showed that closely related cricetid hosts in Europe and North America are infected with closely related Cryptosporidium genotypes. Cricetids were not major reservoirs of human pathogenic Cryptosporidium spp. Article in Journal/Newspaper Microtus arvalis Cambridge University Press Parasitology 145 3 326 334 |
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Open Polar |
collection |
Cambridge University Press |
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crcambridgeupr |
language |
English |
description |
SUMMARY We undertook a study on Cryptosporidium spp. in wild cricetid rodents. Fecal samples were collected from meadow voles ( Microtus pennsylvanicus ), southern red-backed voles ( Myodes gapperi ), woodland voles ( Microtus pinetorum ), muskrats ( Ondatra zibethicus ) and Peromyscus spp. mice in North America, and from bank voles ( Myodes glareolus ) and common voles ( Microtus arvalis ) in Europe. Isolates were characterized by sequence and phylogenetic analyses of the small subunit ribosomal RNA (SSU) and actin genes. Overall, 33·2% (362/1089) of cricetids tested positive for Cryptosporidium , with a greater prevalence in cricetids from North America (50·7%; 302/596) than Europe (12·1%; 60/493). Principal Coordinate analysis separated SSU sequences into three major groups (G1-G3), each represented by sequences from North American and European cricetids. A maximum likelihood tree of SSU sequences had low bootstrap support and showed G1 to be more heterogeneous than G2 or G3. Actin and concatenated actin-SSU trees, which were better resolved and had higher bootstrap support than the SSU phylogeny, showed that closely related cricetid hosts in Europe and North America are infected with closely related Cryptosporidium genotypes. Cricetids were not major reservoirs of human pathogenic Cryptosporidium spp. |
format |
Article in Journal/Newspaper |
author |
STENGER, BRIANNA L. S. HORČIČKOVÁ, MICHAELA CLARK, MARK E. KVÁČ, MARTIN ČONDLOVÁ, ŠÁRKA KHAN, EAKALAK WIDMER, GIOVANNI XIAO, LIHUA GIDDINGS, CATHERINE W. PENNIL, CHRISTOPHER STANKO, MICHAL SAK, BOHUMIL MCEVOY, JOHN M. |
spellingShingle |
STENGER, BRIANNA L. S. HORČIČKOVÁ, MICHAELA CLARK, MARK E. KVÁČ, MARTIN ČONDLOVÁ, ŠÁRKA KHAN, EAKALAK WIDMER, GIOVANNI XIAO, LIHUA GIDDINGS, CATHERINE W. PENNIL, CHRISTOPHER STANKO, MICHAL SAK, BOHUMIL MCEVOY, JOHN M. Cryptosporidiuminfecting wild cricetid rodents from the subfamilies Arvicolinae and Neotominae |
author_facet |
STENGER, BRIANNA L. S. HORČIČKOVÁ, MICHAELA CLARK, MARK E. KVÁČ, MARTIN ČONDLOVÁ, ŠÁRKA KHAN, EAKALAK WIDMER, GIOVANNI XIAO, LIHUA GIDDINGS, CATHERINE W. PENNIL, CHRISTOPHER STANKO, MICHAL SAK, BOHUMIL MCEVOY, JOHN M. |
author_sort |
STENGER, BRIANNA L. S. |
title |
Cryptosporidiuminfecting wild cricetid rodents from the subfamilies Arvicolinae and Neotominae |
title_short |
Cryptosporidiuminfecting wild cricetid rodents from the subfamilies Arvicolinae and Neotominae |
title_full |
Cryptosporidiuminfecting wild cricetid rodents from the subfamilies Arvicolinae and Neotominae |
title_fullStr |
Cryptosporidiuminfecting wild cricetid rodents from the subfamilies Arvicolinae and Neotominae |
title_full_unstemmed |
Cryptosporidiuminfecting wild cricetid rodents from the subfamilies Arvicolinae and Neotominae |
title_sort |
cryptosporidiuminfecting wild cricetid rodents from the subfamilies arvicolinae and neotominae |
publisher |
Cambridge University Press (CUP) |
publishDate |
2017 |
url |
http://dx.doi.org/10.1017/s0031182017001524 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0031182017001524 |
genre |
Microtus arvalis |
genre_facet |
Microtus arvalis |
op_source |
Parasitology volume 145, issue 3, page 326-334 ISSN 0031-1820 1469-8161 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0031182017001524 |
container_title |
Parasitology |
container_volume |
145 |
container_issue |
3 |
container_start_page |
326 |
op_container_end_page |
334 |
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1810456846766440448 |