New data on flatfish scuticociliatosis reveal that Miamiensis avidus and Philasterides dicentrarchi are different species

SUMMARY Scuticociliatosis is a severe disease in farmed flatfish. However, the causative agent is not always accurately identified. In this study, we identified two isolates of scuticociliates from an outbreak in cultured fine flounder Paralichthys adspersus . Scuticociliate identification was based...

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Published in:Parasitology
Main Authors: DE FELIPE, ANA-PAULA, LAMAS, JESÚS, SUEIRO, ROSA-ANA, FOLGUEIRA, IRIA, LEIRO, JOSÉ-MANUEL
Format: Article in Journal/Newspaper
Language:English
Published: Cambridge University Press (CUP) 2017
Subjects:
Online Access:http://dx.doi.org/10.1017/s0031182017000749
https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0031182017000749
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spelling crcambridgeupr:10.1017/s0031182017000749 2024-03-03T08:48:39+00:00 New data on flatfish scuticociliatosis reveal that Miamiensis avidus and Philasterides dicentrarchi are different species DE FELIPE, ANA-PAULA LAMAS, JESÚS SUEIRO, ROSA-ANA FOLGUEIRA, IRIA LEIRO, JOSÉ-MANUEL 2017 http://dx.doi.org/10.1017/s0031182017000749 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0031182017000749 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Parasitology volume 144, issue 10, page 1394-1411 ISSN 0031-1820 1469-8161 Infectious Diseases Animal Science and Zoology Parasitology journal-article 2017 crcambridgeupr https://doi.org/10.1017/s0031182017000749 2024-02-08T08:42:56Z SUMMARY Scuticociliatosis is a severe disease in farmed flatfish. However, the causative agent is not always accurately identified. In this study, we identified two isolates of scuticociliates from an outbreak in cultured fine flounder Paralichthys adspersus . Scuticociliate identification was based on morphological data, examination of life stages and the use of molecular approaches. The isolates were compared with a strain of Philasterides dicentrachi from turbot Scophthalmus maximus and with a strain deposited in the American Type Culture Collection as Miamiensis avidus ATCC ® 50180 ™ . The use of morphological, biological and molecular methods enabled us to identify the isolates from the fine flouder as P. dicentrarchi . Comparison of P. dicentrachi isolates and M. avidus revealed some differences in the buccal apparatus. Unlike P. dicentrarchi, M. avidus has a life cycle with three forms: macrostomes (capable of feeding on P. dicentrarchi ), microstomes and tomites. Additionally, we found differences in the 18S rRNA and α- and β-tubulin gene sequences, indicating that P. dicentrarchi and M. avidus are different species. We therefore reject the synonymy/conspecificity of the two taxa previously suggested. Finally, we suggest that a combination of morphological, biological, molecular (by multigene analysis) and serological techniques could improve the identification of scuticociliates parasites in fish. Article in Journal/Newspaper Scophthalmus maximus Turbot Cambridge University Press Parasitology 144 10 1394 1411
institution Open Polar
collection Cambridge University Press
op_collection_id crcambridgeupr
language English
topic Infectious Diseases
Animal Science and Zoology
Parasitology
spellingShingle Infectious Diseases
Animal Science and Zoology
Parasitology
DE FELIPE, ANA-PAULA
LAMAS, JESÚS
SUEIRO, ROSA-ANA
FOLGUEIRA, IRIA
LEIRO, JOSÉ-MANUEL
New data on flatfish scuticociliatosis reveal that Miamiensis avidus and Philasterides dicentrarchi are different species
topic_facet Infectious Diseases
Animal Science and Zoology
Parasitology
description SUMMARY Scuticociliatosis is a severe disease in farmed flatfish. However, the causative agent is not always accurately identified. In this study, we identified two isolates of scuticociliates from an outbreak in cultured fine flounder Paralichthys adspersus . Scuticociliate identification was based on morphological data, examination of life stages and the use of molecular approaches. The isolates were compared with a strain of Philasterides dicentrachi from turbot Scophthalmus maximus and with a strain deposited in the American Type Culture Collection as Miamiensis avidus ATCC ® 50180 ™ . The use of morphological, biological and molecular methods enabled us to identify the isolates from the fine flouder as P. dicentrarchi . Comparison of P. dicentrachi isolates and M. avidus revealed some differences in the buccal apparatus. Unlike P. dicentrarchi, M. avidus has a life cycle with three forms: macrostomes (capable of feeding on P. dicentrarchi ), microstomes and tomites. Additionally, we found differences in the 18S rRNA and α- and β-tubulin gene sequences, indicating that P. dicentrarchi and M. avidus are different species. We therefore reject the synonymy/conspecificity of the two taxa previously suggested. Finally, we suggest that a combination of morphological, biological, molecular (by multigene analysis) and serological techniques could improve the identification of scuticociliates parasites in fish.
format Article in Journal/Newspaper
author DE FELIPE, ANA-PAULA
LAMAS, JESÚS
SUEIRO, ROSA-ANA
FOLGUEIRA, IRIA
LEIRO, JOSÉ-MANUEL
author_facet DE FELIPE, ANA-PAULA
LAMAS, JESÚS
SUEIRO, ROSA-ANA
FOLGUEIRA, IRIA
LEIRO, JOSÉ-MANUEL
author_sort DE FELIPE, ANA-PAULA
title New data on flatfish scuticociliatosis reveal that Miamiensis avidus and Philasterides dicentrarchi are different species
title_short New data on flatfish scuticociliatosis reveal that Miamiensis avidus and Philasterides dicentrarchi are different species
title_full New data on flatfish scuticociliatosis reveal that Miamiensis avidus and Philasterides dicentrarchi are different species
title_fullStr New data on flatfish scuticociliatosis reveal that Miamiensis avidus and Philasterides dicentrarchi are different species
title_full_unstemmed New data on flatfish scuticociliatosis reveal that Miamiensis avidus and Philasterides dicentrarchi are different species
title_sort new data on flatfish scuticociliatosis reveal that miamiensis avidus and philasterides dicentrarchi are different species
publisher Cambridge University Press (CUP)
publishDate 2017
url http://dx.doi.org/10.1017/s0031182017000749
https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0031182017000749
genre Scophthalmus maximus
Turbot
genre_facet Scophthalmus maximus
Turbot
op_source Parasitology
volume 144, issue 10, page 1394-1411
ISSN 0031-1820 1469-8161
op_rights https://www.cambridge.org/core/terms
op_doi https://doi.org/10.1017/s0031182017000749
container_title Parasitology
container_volume 144
container_issue 10
container_start_page 1394
op_container_end_page 1411
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