New data on flatfish scuticociliatosis reveal that Miamiensis avidus and Philasterides dicentrarchi are different species
SUMMARY Scuticociliatosis is a severe disease in farmed flatfish. However, the causative agent is not always accurately identified. In this study, we identified two isolates of scuticociliates from an outbreak in cultured fine flounder Paralichthys adspersus . Scuticociliate identification was based...
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crcambridgeupr:10.1017/s0031182017000749 2024-03-03T08:48:39+00:00 New data on flatfish scuticociliatosis reveal that Miamiensis avidus and Philasterides dicentrarchi are different species DE FELIPE, ANA-PAULA LAMAS, JESÚS SUEIRO, ROSA-ANA FOLGUEIRA, IRIA LEIRO, JOSÉ-MANUEL 2017 http://dx.doi.org/10.1017/s0031182017000749 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0031182017000749 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Parasitology volume 144, issue 10, page 1394-1411 ISSN 0031-1820 1469-8161 Infectious Diseases Animal Science and Zoology Parasitology journal-article 2017 crcambridgeupr https://doi.org/10.1017/s0031182017000749 2024-02-08T08:42:56Z SUMMARY Scuticociliatosis is a severe disease in farmed flatfish. However, the causative agent is not always accurately identified. In this study, we identified two isolates of scuticociliates from an outbreak in cultured fine flounder Paralichthys adspersus . Scuticociliate identification was based on morphological data, examination of life stages and the use of molecular approaches. The isolates were compared with a strain of Philasterides dicentrachi from turbot Scophthalmus maximus and with a strain deposited in the American Type Culture Collection as Miamiensis avidus ATCC ® 50180 ™ . The use of morphological, biological and molecular methods enabled us to identify the isolates from the fine flouder as P. dicentrarchi . Comparison of P. dicentrachi isolates and M. avidus revealed some differences in the buccal apparatus. Unlike P. dicentrarchi, M. avidus has a life cycle with three forms: macrostomes (capable of feeding on P. dicentrarchi ), microstomes and tomites. Additionally, we found differences in the 18S rRNA and α- and β-tubulin gene sequences, indicating that P. dicentrarchi and M. avidus are different species. We therefore reject the synonymy/conspecificity of the two taxa previously suggested. Finally, we suggest that a combination of morphological, biological, molecular (by multigene analysis) and serological techniques could improve the identification of scuticociliates parasites in fish. Article in Journal/Newspaper Scophthalmus maximus Turbot Cambridge University Press Parasitology 144 10 1394 1411 |
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Cambridge University Press |
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English |
topic |
Infectious Diseases Animal Science and Zoology Parasitology |
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Infectious Diseases Animal Science and Zoology Parasitology DE FELIPE, ANA-PAULA LAMAS, JESÚS SUEIRO, ROSA-ANA FOLGUEIRA, IRIA LEIRO, JOSÉ-MANUEL New data on flatfish scuticociliatosis reveal that Miamiensis avidus and Philasterides dicentrarchi are different species |
topic_facet |
Infectious Diseases Animal Science and Zoology Parasitology |
description |
SUMMARY Scuticociliatosis is a severe disease in farmed flatfish. However, the causative agent is not always accurately identified. In this study, we identified two isolates of scuticociliates from an outbreak in cultured fine flounder Paralichthys adspersus . Scuticociliate identification was based on morphological data, examination of life stages and the use of molecular approaches. The isolates were compared with a strain of Philasterides dicentrachi from turbot Scophthalmus maximus and with a strain deposited in the American Type Culture Collection as Miamiensis avidus ATCC ® 50180 ™ . The use of morphological, biological and molecular methods enabled us to identify the isolates from the fine flouder as P. dicentrarchi . Comparison of P. dicentrachi isolates and M. avidus revealed some differences in the buccal apparatus. Unlike P. dicentrarchi, M. avidus has a life cycle with three forms: macrostomes (capable of feeding on P. dicentrarchi ), microstomes and tomites. Additionally, we found differences in the 18S rRNA and α- and β-tubulin gene sequences, indicating that P. dicentrarchi and M. avidus are different species. We therefore reject the synonymy/conspecificity of the two taxa previously suggested. Finally, we suggest that a combination of morphological, biological, molecular (by multigene analysis) and serological techniques could improve the identification of scuticociliates parasites in fish. |
format |
Article in Journal/Newspaper |
author |
DE FELIPE, ANA-PAULA LAMAS, JESÚS SUEIRO, ROSA-ANA FOLGUEIRA, IRIA LEIRO, JOSÉ-MANUEL |
author_facet |
DE FELIPE, ANA-PAULA LAMAS, JESÚS SUEIRO, ROSA-ANA FOLGUEIRA, IRIA LEIRO, JOSÉ-MANUEL |
author_sort |
DE FELIPE, ANA-PAULA |
title |
New data on flatfish scuticociliatosis reveal that Miamiensis avidus and Philasterides dicentrarchi are different species |
title_short |
New data on flatfish scuticociliatosis reveal that Miamiensis avidus and Philasterides dicentrarchi are different species |
title_full |
New data on flatfish scuticociliatosis reveal that Miamiensis avidus and Philasterides dicentrarchi are different species |
title_fullStr |
New data on flatfish scuticociliatosis reveal that Miamiensis avidus and Philasterides dicentrarchi are different species |
title_full_unstemmed |
New data on flatfish scuticociliatosis reveal that Miamiensis avidus and Philasterides dicentrarchi are different species |
title_sort |
new data on flatfish scuticociliatosis reveal that miamiensis avidus and philasterides dicentrarchi are different species |
publisher |
Cambridge University Press (CUP) |
publishDate |
2017 |
url |
http://dx.doi.org/10.1017/s0031182017000749 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0031182017000749 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
Parasitology volume 144, issue 10, page 1394-1411 ISSN 0031-1820 1469-8161 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0031182017000749 |
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Parasitology |
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144 |
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10 |
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1394 |
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1411 |
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1792505615928524800 |