Fast Real-Time PCR assay for detection of Tetramicra brevifilum in cultured turbot
SUMMARY Global aquaculture production of turbot has rapidly increased worldwide in the last decade and it is expected to have even bigger growth in the next years due to new farms operating. The losses caused by pathogen infections have grown at the same time as the production of this species. Paras...
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Online Access: | http://dx.doi.org/10.1017/s0031182012001655 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0031182012001655 |
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crcambridgeupr:10.1017/s0031182012001655 2024-03-03T08:49:15+00:00 Fast Real-Time PCR assay for detection of Tetramicra brevifilum in cultured turbot ALONSO, MERCEDES LAGO, FÁTIMA C. GÓMEZ-REINO, MARÍA FERNÁNDEZ, JACOBO MARTÍN, IRIS VIEITES, JUAN M. ESPIÑEIRA, MONTSERRAT 2012 http://dx.doi.org/10.1017/s0031182012001655 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0031182012001655 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Parasitology volume 140, issue 3, page 338-342 ISSN 0031-1820 1469-8161 Infectious Diseases Animal Science and Zoology Parasitology journal-article 2012 crcambridgeupr https://doi.org/10.1017/s0031182012001655 2024-02-08T08:45:37Z SUMMARY Global aquaculture production of turbot has rapidly increased worldwide in the last decade and it is expected to have even bigger growth in the next years due to new farms operating. The losses caused by pathogen infections have grown at the same time as the production of this species. Parasitological infections are among the main relevant pathologies associated with its culture and produce serious losses in aquaculture, reduce the growth rate in fish and may lead to unmarketable fish due to skeletal muscle abnormalities in cases with high intensity of infection. The microsporidian parasite Tetramicra brevifilum causes severe infections and generates major losses in farmed turbot. Infections are difficult to control due to spore longevity and its direct transmission. To facilitate the infection management, an effective tool for fast detection and identification of T. brevifilum is needed. This study provides a molecular methodology of fast Real-Time PCR for T. brevifilum detection to the aquaculture industry, useful for routine control of T. brevifilum at turbot farms. The method is characterized by its high specificity and sensitivity, and it can be applied to cultured turbot for parasite detection regardless of the life-cycle stage of the pathogen or the infection intensity. Article in Journal/Newspaper Turbot Cambridge University Press Parasitology 140 3 338 342 |
institution |
Open Polar |
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Cambridge University Press |
op_collection_id |
crcambridgeupr |
language |
English |
topic |
Infectious Diseases Animal Science and Zoology Parasitology |
spellingShingle |
Infectious Diseases Animal Science and Zoology Parasitology ALONSO, MERCEDES LAGO, FÁTIMA C. GÓMEZ-REINO, MARÍA FERNÁNDEZ, JACOBO MARTÍN, IRIS VIEITES, JUAN M. ESPIÑEIRA, MONTSERRAT Fast Real-Time PCR assay for detection of Tetramicra brevifilum in cultured turbot |
topic_facet |
Infectious Diseases Animal Science and Zoology Parasitology |
description |
SUMMARY Global aquaculture production of turbot has rapidly increased worldwide in the last decade and it is expected to have even bigger growth in the next years due to new farms operating. The losses caused by pathogen infections have grown at the same time as the production of this species. Parasitological infections are among the main relevant pathologies associated with its culture and produce serious losses in aquaculture, reduce the growth rate in fish and may lead to unmarketable fish due to skeletal muscle abnormalities in cases with high intensity of infection. The microsporidian parasite Tetramicra brevifilum causes severe infections and generates major losses in farmed turbot. Infections are difficult to control due to spore longevity and its direct transmission. To facilitate the infection management, an effective tool for fast detection and identification of T. brevifilum is needed. This study provides a molecular methodology of fast Real-Time PCR for T. brevifilum detection to the aquaculture industry, useful for routine control of T. brevifilum at turbot farms. The method is characterized by its high specificity and sensitivity, and it can be applied to cultured turbot for parasite detection regardless of the life-cycle stage of the pathogen or the infection intensity. |
format |
Article in Journal/Newspaper |
author |
ALONSO, MERCEDES LAGO, FÁTIMA C. GÓMEZ-REINO, MARÍA FERNÁNDEZ, JACOBO MARTÍN, IRIS VIEITES, JUAN M. ESPIÑEIRA, MONTSERRAT |
author_facet |
ALONSO, MERCEDES LAGO, FÁTIMA C. GÓMEZ-REINO, MARÍA FERNÁNDEZ, JACOBO MARTÍN, IRIS VIEITES, JUAN M. ESPIÑEIRA, MONTSERRAT |
author_sort |
ALONSO, MERCEDES |
title |
Fast Real-Time PCR assay for detection of Tetramicra brevifilum in cultured turbot |
title_short |
Fast Real-Time PCR assay for detection of Tetramicra brevifilum in cultured turbot |
title_full |
Fast Real-Time PCR assay for detection of Tetramicra brevifilum in cultured turbot |
title_fullStr |
Fast Real-Time PCR assay for detection of Tetramicra brevifilum in cultured turbot |
title_full_unstemmed |
Fast Real-Time PCR assay for detection of Tetramicra brevifilum in cultured turbot |
title_sort |
fast real-time pcr assay for detection of tetramicra brevifilum in cultured turbot |
publisher |
Cambridge University Press (CUP) |
publishDate |
2012 |
url |
http://dx.doi.org/10.1017/s0031182012001655 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0031182012001655 |
genre |
Turbot |
genre_facet |
Turbot |
op_source |
Parasitology volume 140, issue 3, page 338-342 ISSN 0031-1820 1469-8161 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0031182012001655 |
container_title |
Parasitology |
container_volume |
140 |
container_issue |
3 |
container_start_page |
338 |
op_container_end_page |
342 |
_version_ |
1792506437871599616 |