Isolation and culture of Sphaerothecum destruens from Sunbleak ( Leucaspius delineatus) in the UK and pathogenicity experiments in Atlantic salmon ( Salmo salar)
SUMMARY The sunbleak ( Leucaspius delineatus ), a cyprinid fish native to continental Europe and now established in the UK, is experiencing population decline which appears to be linked to the spread of the invasive Asian cyprinid ( Pseudorasbora parva ). A population of sunbleak in the UK has previ...
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Cambridge University Press (CUP)
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Online Access: | http://dx.doi.org/10.1017/s0031182012000030 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0031182012000030 |
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crcambridgeupr:10.1017/s0031182012000030 2024-09-15T17:55:59+00:00 Isolation and culture of Sphaerothecum destruens from Sunbleak ( Leucaspius delineatus) in the UK and pathogenicity experiments in Atlantic salmon ( Salmo salar) PALEY, RICHARD K. ANDREOU, DEMETRA BATEMAN, KELLY S. FEIST, STEPHEN W. 2012 http://dx.doi.org/10.1017/s0031182012000030 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0031182012000030 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Parasitology volume 139, issue 7, page 904-914 ISSN 0031-1820 1469-8161 journal-article 2012 crcambridgeupr https://doi.org/10.1017/s0031182012000030 2024-08-14T04:03:18Z SUMMARY The sunbleak ( Leucaspius delineatus ), a cyprinid fish native to continental Europe and now established in the UK, is experiencing population decline which appears to be linked to the spread of the invasive Asian cyprinid ( Pseudorasbora parva ). A population of sunbleak in the UK has previously been identified as infected with S. destruens at low prevalence. Because Sphaerothaecum destruens has, on occasion, caused severe disease in cultured and wild salmonids the aim of this work was to establish laboratory cultures of S. destruens from sunbleak in the UK and use these cultures in challenge experiments to determine if the UK isolate of S. destruens from cyprinid species is a potential threat to Atlantic salmon ( Salmo salar ). The first isolation and culture of S. destruens in the UK and from a cyprinid species is described. Cultured S. destruens spores from sunbleak are infective to EPC, CHSE and FHM cells, replicating most rapidly in FHM and EPC cells. Spores can be induced to zoosporulate in water forming motile, uni-flagellated zoospores. Challenge experiments indicated the spores are able to replicate and disperse in Atlantic salmon and are associated with increased mortality (up to 90%) when injected intraperitonealy. Article in Journal/Newspaper Atlantic salmon Salmo salar Cambridge University Press Parasitology 139 7 904 914 |
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Open Polar |
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Cambridge University Press |
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crcambridgeupr |
language |
English |
description |
SUMMARY The sunbleak ( Leucaspius delineatus ), a cyprinid fish native to continental Europe and now established in the UK, is experiencing population decline which appears to be linked to the spread of the invasive Asian cyprinid ( Pseudorasbora parva ). A population of sunbleak in the UK has previously been identified as infected with S. destruens at low prevalence. Because Sphaerothaecum destruens has, on occasion, caused severe disease in cultured and wild salmonids the aim of this work was to establish laboratory cultures of S. destruens from sunbleak in the UK and use these cultures in challenge experiments to determine if the UK isolate of S. destruens from cyprinid species is a potential threat to Atlantic salmon ( Salmo salar ). The first isolation and culture of S. destruens in the UK and from a cyprinid species is described. Cultured S. destruens spores from sunbleak are infective to EPC, CHSE and FHM cells, replicating most rapidly in FHM and EPC cells. Spores can be induced to zoosporulate in water forming motile, uni-flagellated zoospores. Challenge experiments indicated the spores are able to replicate and disperse in Atlantic salmon and are associated with increased mortality (up to 90%) when injected intraperitonealy. |
format |
Article in Journal/Newspaper |
author |
PALEY, RICHARD K. ANDREOU, DEMETRA BATEMAN, KELLY S. FEIST, STEPHEN W. |
spellingShingle |
PALEY, RICHARD K. ANDREOU, DEMETRA BATEMAN, KELLY S. FEIST, STEPHEN W. Isolation and culture of Sphaerothecum destruens from Sunbleak ( Leucaspius delineatus) in the UK and pathogenicity experiments in Atlantic salmon ( Salmo salar) |
author_facet |
PALEY, RICHARD K. ANDREOU, DEMETRA BATEMAN, KELLY S. FEIST, STEPHEN W. |
author_sort |
PALEY, RICHARD K. |
title |
Isolation and culture of Sphaerothecum destruens from Sunbleak ( Leucaspius delineatus) in the UK and pathogenicity experiments in Atlantic salmon ( Salmo salar) |
title_short |
Isolation and culture of Sphaerothecum destruens from Sunbleak ( Leucaspius delineatus) in the UK and pathogenicity experiments in Atlantic salmon ( Salmo salar) |
title_full |
Isolation and culture of Sphaerothecum destruens from Sunbleak ( Leucaspius delineatus) in the UK and pathogenicity experiments in Atlantic salmon ( Salmo salar) |
title_fullStr |
Isolation and culture of Sphaerothecum destruens from Sunbleak ( Leucaspius delineatus) in the UK and pathogenicity experiments in Atlantic salmon ( Salmo salar) |
title_full_unstemmed |
Isolation and culture of Sphaerothecum destruens from Sunbleak ( Leucaspius delineatus) in the UK and pathogenicity experiments in Atlantic salmon ( Salmo salar) |
title_sort |
isolation and culture of sphaerothecum destruens from sunbleak ( leucaspius delineatus) in the uk and pathogenicity experiments in atlantic salmon ( salmo salar) |
publisher |
Cambridge University Press (CUP) |
publishDate |
2012 |
url |
http://dx.doi.org/10.1017/s0031182012000030 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0031182012000030 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Parasitology volume 139, issue 7, page 904-914 ISSN 0031-1820 1469-8161 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0031182012000030 |
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Parasitology |
container_volume |
139 |
container_issue |
7 |
container_start_page |
904 |
op_container_end_page |
914 |
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1810432203478269952 |