SSCP-based identification of members within the Pseudoterranova decipiens complex (Nematoda: Ascaridoidea: Anisakidae) using genetic markers in the internal transcribed spacers of ribosomal DNA
The anisakid nematodes morphologically corresponding with Pseudoterranova decipiens sensu lato ( s.l. ) (Krabbe, 1878) from different seal or sea lion hosts and geographical origins, previously identified as Pseudoterranova krabbei , P. decipiens ( s.s. ), P. bulbosa , P. azarasi and P. cattani by m...
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crcambridgeupr:10.1017/s0031182002001579 2024-06-16T07:35:22+00:00 SSCP-based identification of members within the Pseudoterranova decipiens complex (Nematoda: Ascaridoidea: Anisakidae) using genetic markers in the internal transcribed spacers of ribosomal DNA ZHU, X. Q. D'AMELIO, S. PALM, H. W. PAGGI, L. GEORGE-NASCIMENTO, M. GASSER, R. B. 2002 http://dx.doi.org/10.1017/s0031182002001579 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0031182002001579 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Parasitology volume 124, issue 6, page 615-623 ISSN 0031-1820 1469-8161 journal-article 2002 crcambridgeupr https://doi.org/10.1017/s0031182002001579 2024-05-22T12:56:31Z The anisakid nematodes morphologically corresponding with Pseudoterranova decipiens sensu lato ( s.l. ) (Krabbe, 1878) from different seal or sea lion hosts and geographical origins, previously identified as Pseudoterranova krabbei , P. decipiens ( s.s. ), P. bulbosa , P. azarasi and P. cattani by multilocus enzyme electrophoresis, were characterized using a DNA approach. Also a population of P. decipiens ( s.l. ) from Chaenocephalus aceratus , the blackfin icefish, from Antarctica and another from Osmerus eperlanus , the European smelt, from Germany were included in the study. The first (ITS-1) and second (ITS-2) internal transcribed spacers (ITS) of ribosomal DNA (rDNA) were amplified by PCR from individual nematodes and analysed by single-strand conformation polymorphism (SSCP), followed by selective sequencing. While no variation in single-stranded ITS-1 and ITS-2 profiles was detected among samples representing each of the species or populations (with the exception of slight microheterogeneity), SSCP analysis of the ITS-2 amplicons allowed the unequivocal differentiation of all of the 5 sibling species of P. decipiens ( s.l. ) examined, which was supported by sequence differences in ITS rDNA. Samples representing the P. decipiens ( s.l. ) population from O. eperlanus had the same SSCP profile as those of P. decipiens ( s.s. ), which was supported by a lack of nucleotide difference in the ITS between them, suggesting that the former represented P. decipiens ( s.s. ). Based on SSCP results and ITS sequence data, P. decipiens ( s.l. ) from C. aceratus was genetically most distinct with respect to all other members of Pseudoterranova examined, which indicated that it may represent P. decipiens E (based on geographical origin) or a distinct species. These findings and the molecular approach taken should have important implications for studying the life-cycles, transmission patterns, epidemiology and population genetics of these anisakid nematodes, and the diagnosis of their infections. Article in Journal/Newspaper Antarc* Antarctica Icefish Cambridge University Press Parasitology 124 6 615 623 |
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Cambridge University Press |
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English |
description |
The anisakid nematodes morphologically corresponding with Pseudoterranova decipiens sensu lato ( s.l. ) (Krabbe, 1878) from different seal or sea lion hosts and geographical origins, previously identified as Pseudoterranova krabbei , P. decipiens ( s.s. ), P. bulbosa , P. azarasi and P. cattani by multilocus enzyme electrophoresis, were characterized using a DNA approach. Also a population of P. decipiens ( s.l. ) from Chaenocephalus aceratus , the blackfin icefish, from Antarctica and another from Osmerus eperlanus , the European smelt, from Germany were included in the study. The first (ITS-1) and second (ITS-2) internal transcribed spacers (ITS) of ribosomal DNA (rDNA) were amplified by PCR from individual nematodes and analysed by single-strand conformation polymorphism (SSCP), followed by selective sequencing. While no variation in single-stranded ITS-1 and ITS-2 profiles was detected among samples representing each of the species or populations (with the exception of slight microheterogeneity), SSCP analysis of the ITS-2 amplicons allowed the unequivocal differentiation of all of the 5 sibling species of P. decipiens ( s.l. ) examined, which was supported by sequence differences in ITS rDNA. Samples representing the P. decipiens ( s.l. ) population from O. eperlanus had the same SSCP profile as those of P. decipiens ( s.s. ), which was supported by a lack of nucleotide difference in the ITS between them, suggesting that the former represented P. decipiens ( s.s. ). Based on SSCP results and ITS sequence data, P. decipiens ( s.l. ) from C. aceratus was genetically most distinct with respect to all other members of Pseudoterranova examined, which indicated that it may represent P. decipiens E (based on geographical origin) or a distinct species. These findings and the molecular approach taken should have important implications for studying the life-cycles, transmission patterns, epidemiology and population genetics of these anisakid nematodes, and the diagnosis of their infections. |
format |
Article in Journal/Newspaper |
author |
ZHU, X. Q. D'AMELIO, S. PALM, H. W. PAGGI, L. GEORGE-NASCIMENTO, M. GASSER, R. B. |
spellingShingle |
ZHU, X. Q. D'AMELIO, S. PALM, H. W. PAGGI, L. GEORGE-NASCIMENTO, M. GASSER, R. B. SSCP-based identification of members within the Pseudoterranova decipiens complex (Nematoda: Ascaridoidea: Anisakidae) using genetic markers in the internal transcribed spacers of ribosomal DNA |
author_facet |
ZHU, X. Q. D'AMELIO, S. PALM, H. W. PAGGI, L. GEORGE-NASCIMENTO, M. GASSER, R. B. |
author_sort |
ZHU, X. Q. |
title |
SSCP-based identification of members within the Pseudoterranova decipiens complex (Nematoda: Ascaridoidea: Anisakidae) using genetic markers in the internal transcribed spacers of ribosomal DNA |
title_short |
SSCP-based identification of members within the Pseudoterranova decipiens complex (Nematoda: Ascaridoidea: Anisakidae) using genetic markers in the internal transcribed spacers of ribosomal DNA |
title_full |
SSCP-based identification of members within the Pseudoterranova decipiens complex (Nematoda: Ascaridoidea: Anisakidae) using genetic markers in the internal transcribed spacers of ribosomal DNA |
title_fullStr |
SSCP-based identification of members within the Pseudoterranova decipiens complex (Nematoda: Ascaridoidea: Anisakidae) using genetic markers in the internal transcribed spacers of ribosomal DNA |
title_full_unstemmed |
SSCP-based identification of members within the Pseudoterranova decipiens complex (Nematoda: Ascaridoidea: Anisakidae) using genetic markers in the internal transcribed spacers of ribosomal DNA |
title_sort |
sscp-based identification of members within the pseudoterranova decipiens complex (nematoda: ascaridoidea: anisakidae) using genetic markers in the internal transcribed spacers of ribosomal dna |
publisher |
Cambridge University Press (CUP) |
publishDate |
2002 |
url |
http://dx.doi.org/10.1017/s0031182002001579 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0031182002001579 |
genre |
Antarc* Antarctica Icefish |
genre_facet |
Antarc* Antarctica Icefish |
op_source |
Parasitology volume 124, issue 6, page 615-623 ISSN 0031-1820 1469-8161 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0031182002001579 |
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Parasitology |
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124 |
container_issue |
6 |
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615 |
op_container_end_page |
623 |
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1802012107775934464 |