Cuticle production and ecdysis in larval marine ascaridoid nematodes in vitro

SUMMARY Infective 3rd-stage larvae of three species of marine ascaridoid nematodes, Pseudoterranova (= Phocanema = Porrocaecum = Terranova ) decipiens, Anisakis simplex and Phocascaris/Contracaecum sp. were cultured in vitro in 0·9% saline or Medium 199 at 37°C beneath an atmosphere of either air or...

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Bibliographic Details
Published in:Parasitology
Main Authors: Mercer, J. G., Munn, A. E., Smith, J. W., Rees, H. H.
Format: Article in Journal/Newspaper
Language:English
Published: Cambridge University Press (CUP) 1986
Subjects:
Infectious Diseases
Animal Science and Zoology
Parasitology
Terranova
Online Access:http://dx.doi.org/10.1017/s0031182000065562
https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0031182000065562
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Summary:SUMMARY Infective 3rd-stage larvae of three species of marine ascaridoid nematodes, Pseudoterranova (= Phocanema = Porrocaecum = Terranova ) decipiens, Anisakis simplex and Phocascaris/Contracaecum sp. were cultured in vitro in 0·9% saline or Medium 199 at 37°C beneath an atmosphere of either air or 95% air:5% CO 2 All three species responded to culture at 37°C by producing a new cuticle. The majority of P. decipiens completed ecdysis under all the culture conditions employed. Ecdysis in A. simplex was stimulated by a high concentration of CO 2 this effect was particularly noticeable in saline cultures. Both A. simplex and Phocascaris/Contracaecum sp. developed more effectively in a nutrient medium than in saline.

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