Anisakids (Nematoda: Anisakidae) from ringed seal, Pusa hispida , and bearded seal, Erignathus barbatus (Mammalia: Pinnipedia) from Nunavut region
As many Arctic fish species are intermediate hosts of anisakids, they are present in the diet of the ringed seal, Pusa hispida , and the bearded seal, Erignathus barbatus . Parasitic nematodes from the stomachs of 66 seals caught in the Nunavut region (Canada) from two communities (Arviat and Saniki...
Published in: | Journal of the Marine Biological Association of the United Kingdom |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Cambridge University Press (CUP)
2013
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1017/s0025315413001276 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0025315413001276 |
id |
crcambridgeupr:10.1017/s0025315413001276 |
---|---|
record_format |
openpolar |
spelling |
crcambridgeupr:10.1017/s0025315413001276 2024-03-03T08:42:09+00:00 Anisakids (Nematoda: Anisakidae) from ringed seal, Pusa hispida , and bearded seal, Erignathus barbatus (Mammalia: Pinnipedia) from Nunavut region Karpiej, Katarzyna Simard, Manon Pufall, Erica Rokicki, Jerzy 2013 http://dx.doi.org/10.1017/s0025315413001276 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0025315413001276 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Journal of the Marine Biological Association of the United Kingdom volume 94, issue 6, page 1237-1241 ISSN 0025-3154 1469-7769 Aquatic Science journal-article 2013 crcambridgeupr https://doi.org/10.1017/s0025315413001276 2024-02-08T08:42:06Z As many Arctic fish species are intermediate hosts of anisakids, they are present in the diet of the ringed seal, Pusa hispida , and the bearded seal, Erignathus barbatus . Parasitic nematodes from the stomachs of 66 seals caught in the Nunavut region (Canada) from two communities (Arviat and Sanikiluaq) from October 2007 to January 2008 have been examined in order to identify the epidemiological risk for Inuit communities who consume traditional food. In Arviat 2428 anisakids were observed in 37 seals, while in Sanikiluaq 316 Anisakidae were isolated from 29 seals. The worms were treated with a host tissue, washed in deionized water and stored until analysis in 70% ethanol. The parasites were divided into three parts. The anterior and posterior parts were stored in 70% ethanol containing 5% glycerol and were examined using a light microscope by evaporation of the ethanol/glycerin mixture. The central parts were prepared for molecular identification by fixing in 70% ethanol. Using the polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) method, the following members of the family Anisakidae were identified: Contracaecum osculatum A and C and Pseudoterranova bulbosa . In the studied material, more adult worms were noted than larval stages. The most numerous nematodes were P. bulbosa , and mixed infection was observed. The mean prevalence of anisakids infection was 43.2% in the Arviat and 37.9% in the Sanikiluaq communities. Article in Journal/Newspaper Arctic Arviat bearded seal Erignathus barbatus inuit Nunavut Pusa hispida ringed seal Sanikiluaq Cambridge University Press Arctic Nunavut Canada Sanikiluaq ENVELOPE(-79.226,-79.226,56.541,56.541) Journal of the Marine Biological Association of the United Kingdom 94 6 1237 1241 |
institution |
Open Polar |
collection |
Cambridge University Press |
op_collection_id |
crcambridgeupr |
language |
English |
topic |
Aquatic Science |
spellingShingle |
Aquatic Science Karpiej, Katarzyna Simard, Manon Pufall, Erica Rokicki, Jerzy Anisakids (Nematoda: Anisakidae) from ringed seal, Pusa hispida , and bearded seal, Erignathus barbatus (Mammalia: Pinnipedia) from Nunavut region |
topic_facet |
Aquatic Science |
description |
As many Arctic fish species are intermediate hosts of anisakids, they are present in the diet of the ringed seal, Pusa hispida , and the bearded seal, Erignathus barbatus . Parasitic nematodes from the stomachs of 66 seals caught in the Nunavut region (Canada) from two communities (Arviat and Sanikiluaq) from October 2007 to January 2008 have been examined in order to identify the epidemiological risk for Inuit communities who consume traditional food. In Arviat 2428 anisakids were observed in 37 seals, while in Sanikiluaq 316 Anisakidae were isolated from 29 seals. The worms were treated with a host tissue, washed in deionized water and stored until analysis in 70% ethanol. The parasites were divided into three parts. The anterior and posterior parts were stored in 70% ethanol containing 5% glycerol and were examined using a light microscope by evaporation of the ethanol/glycerin mixture. The central parts were prepared for molecular identification by fixing in 70% ethanol. Using the polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) method, the following members of the family Anisakidae were identified: Contracaecum osculatum A and C and Pseudoterranova bulbosa . In the studied material, more adult worms were noted than larval stages. The most numerous nematodes were P. bulbosa , and mixed infection was observed. The mean prevalence of anisakids infection was 43.2% in the Arviat and 37.9% in the Sanikiluaq communities. |
format |
Article in Journal/Newspaper |
author |
Karpiej, Katarzyna Simard, Manon Pufall, Erica Rokicki, Jerzy |
author_facet |
Karpiej, Katarzyna Simard, Manon Pufall, Erica Rokicki, Jerzy |
author_sort |
Karpiej, Katarzyna |
title |
Anisakids (Nematoda: Anisakidae) from ringed seal, Pusa hispida , and bearded seal, Erignathus barbatus (Mammalia: Pinnipedia) from Nunavut region |
title_short |
Anisakids (Nematoda: Anisakidae) from ringed seal, Pusa hispida , and bearded seal, Erignathus barbatus (Mammalia: Pinnipedia) from Nunavut region |
title_full |
Anisakids (Nematoda: Anisakidae) from ringed seal, Pusa hispida , and bearded seal, Erignathus barbatus (Mammalia: Pinnipedia) from Nunavut region |
title_fullStr |
Anisakids (Nematoda: Anisakidae) from ringed seal, Pusa hispida , and bearded seal, Erignathus barbatus (Mammalia: Pinnipedia) from Nunavut region |
title_full_unstemmed |
Anisakids (Nematoda: Anisakidae) from ringed seal, Pusa hispida , and bearded seal, Erignathus barbatus (Mammalia: Pinnipedia) from Nunavut region |
title_sort |
anisakids (nematoda: anisakidae) from ringed seal, pusa hispida , and bearded seal, erignathus barbatus (mammalia: pinnipedia) from nunavut region |
publisher |
Cambridge University Press (CUP) |
publishDate |
2013 |
url |
http://dx.doi.org/10.1017/s0025315413001276 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0025315413001276 |
long_lat |
ENVELOPE(-79.226,-79.226,56.541,56.541) |
geographic |
Arctic Nunavut Canada Sanikiluaq |
geographic_facet |
Arctic Nunavut Canada Sanikiluaq |
genre |
Arctic Arviat bearded seal Erignathus barbatus inuit Nunavut Pusa hispida ringed seal Sanikiluaq |
genre_facet |
Arctic Arviat bearded seal Erignathus barbatus inuit Nunavut Pusa hispida ringed seal Sanikiluaq |
op_source |
Journal of the Marine Biological Association of the United Kingdom volume 94, issue 6, page 1237-1241 ISSN 0025-3154 1469-7769 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0025315413001276 |
container_title |
Journal of the Marine Biological Association of the United Kingdom |
container_volume |
94 |
container_issue |
6 |
container_start_page |
1237 |
op_container_end_page |
1241 |
_version_ |
1792497630449762304 |