The impact of stress on cytokine and haptoglobin mRNA expression in blood samples from harbour porpoises ( Phocoena phocoena)
Cytokines are important cell mediators involved in immune responses. Their expression can be modulated by numerous factors, including stress. The aim of this study was to compare cytokine mRNA expression from harbour porpoises exposed to different environments. Blood samples were taken from two heal...
Published in: | Journal of the Marine Biological Association of the United Kingdom |
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Cambridge University Press (CUP)
2007
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Online Access: | http://dx.doi.org/10.1017/s0025315407055567 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0025315407055567 |
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crcambridgeupr:10.1017/s0025315407055567 2024-09-09T20:03:34+00:00 The impact of stress on cytokine and haptoglobin mRNA expression in blood samples from harbour porpoises ( Phocoena phocoena) Fonfara, S. Siebert, U. Prange, A. Colijn, F. 2007 http://dx.doi.org/10.1017/s0025315407055567 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0025315407055567 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Journal of the Marine Biological Association of the United Kingdom volume 87, issue 1, page 305-311 ISSN 0025-3154 1469-7769 journal-article 2007 crcambridgeupr https://doi.org/10.1017/s0025315407055567 2024-06-19T04:04:21Z Cytokines are important cell mediators involved in immune responses. Their expression can be modulated by numerous factors, including stress. The aim of this study was to compare cytokine mRNA expression from harbour porpoises exposed to different environments. Blood samples were taken from two healthy porpoises living in captivity at the Fjord and Belt Centre Kerteminde, Denmark, and from four wild porpoises accidentally caught in Danish waters. Real-time RT-PCR was used to quantify the transcription of interleukin-(IL)-1β, IL-2, -4, -6, -10, tumour necrosis factor-(TNF)-α, transforming growth factor-(TGF)-β and the acute phase protein haptoglobin. This revealed downregulation of the pro-inflammatory cytokines IL-1β, IL-2, IL-6 and TNFα, and a switch towards the T-helper-cell-(Th)2- and Th3-cytokines, IL-4 and TGFβ, in blood samples of the wild-captured animals. This indicated a shift towards immunomodulatory cytokines. In addition, cortisol levels were increased in the wild-caught porpoises. These results are suggestive of stress-induced modulation of the immune responses in the accidentally caught animals. The current study indicates that the expression pattern of these cytokines and the estimation of the Th1- to Th2- and Th3-cytokine mRNA ratios might be a useful indicator to analyse the influence of stress on the immune system in harbour porpoises. Article in Journal/Newspaper Phocoena phocoena Cambridge University Press Journal of the Marine Biological Association of the United Kingdom 87 1 305 311 |
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Open Polar |
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Cambridge University Press |
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crcambridgeupr |
language |
English |
description |
Cytokines are important cell mediators involved in immune responses. Their expression can be modulated by numerous factors, including stress. The aim of this study was to compare cytokine mRNA expression from harbour porpoises exposed to different environments. Blood samples were taken from two healthy porpoises living in captivity at the Fjord and Belt Centre Kerteminde, Denmark, and from four wild porpoises accidentally caught in Danish waters. Real-time RT-PCR was used to quantify the transcription of interleukin-(IL)-1β, IL-2, -4, -6, -10, tumour necrosis factor-(TNF)-α, transforming growth factor-(TGF)-β and the acute phase protein haptoglobin. This revealed downregulation of the pro-inflammatory cytokines IL-1β, IL-2, IL-6 and TNFα, and a switch towards the T-helper-cell-(Th)2- and Th3-cytokines, IL-4 and TGFβ, in blood samples of the wild-captured animals. This indicated a shift towards immunomodulatory cytokines. In addition, cortisol levels were increased in the wild-caught porpoises. These results are suggestive of stress-induced modulation of the immune responses in the accidentally caught animals. The current study indicates that the expression pattern of these cytokines and the estimation of the Th1- to Th2- and Th3-cytokine mRNA ratios might be a useful indicator to analyse the influence of stress on the immune system in harbour porpoises. |
format |
Article in Journal/Newspaper |
author |
Fonfara, S. Siebert, U. Prange, A. Colijn, F. |
spellingShingle |
Fonfara, S. Siebert, U. Prange, A. Colijn, F. The impact of stress on cytokine and haptoglobin mRNA expression in blood samples from harbour porpoises ( Phocoena phocoena) |
author_facet |
Fonfara, S. Siebert, U. Prange, A. Colijn, F. |
author_sort |
Fonfara, S. |
title |
The impact of stress on cytokine and haptoglobin mRNA expression in blood samples from harbour porpoises ( Phocoena phocoena) |
title_short |
The impact of stress on cytokine and haptoglobin mRNA expression in blood samples from harbour porpoises ( Phocoena phocoena) |
title_full |
The impact of stress on cytokine and haptoglobin mRNA expression in blood samples from harbour porpoises ( Phocoena phocoena) |
title_fullStr |
The impact of stress on cytokine and haptoglobin mRNA expression in blood samples from harbour porpoises ( Phocoena phocoena) |
title_full_unstemmed |
The impact of stress on cytokine and haptoglobin mRNA expression in blood samples from harbour porpoises ( Phocoena phocoena) |
title_sort |
impact of stress on cytokine and haptoglobin mrna expression in blood samples from harbour porpoises ( phocoena phocoena) |
publisher |
Cambridge University Press (CUP) |
publishDate |
2007 |
url |
http://dx.doi.org/10.1017/s0025315407055567 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0025315407055567 |
genre |
Phocoena phocoena |
genre_facet |
Phocoena phocoena |
op_source |
Journal of the Marine Biological Association of the United Kingdom volume 87, issue 1, page 305-311 ISSN 0025-3154 1469-7769 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0025315407055567 |
container_title |
Journal of the Marine Biological Association of the United Kingdom |
container_volume |
87 |
container_issue |
1 |
container_start_page |
305 |
op_container_end_page |
311 |
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1809935532680019968 |