Viral impacts upon marine bacterioplankton assemblage structure
This study examined the relationship between viral infection and the richness, diversity and composition of bacterial assemblages in the water column. Viruses were enriched by ultrafiltration, added to water column incubation experiments at 15 locations in the North Atlantic, North Pacific, Gulf of...
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Online Access: | http://dx.doi.org/10.1017/s002531540601349x https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S002531540601349X |
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crcambridgeupr:10.1017/s002531540601349x 2024-09-15T18:23:44+00:00 Viral impacts upon marine bacterioplankton assemblage structure Hewson, Ian Fuhrman, Jed A. 2006 http://dx.doi.org/10.1017/s002531540601349x https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S002531540601349X en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Journal of the Marine Biological Association of the United Kingdom volume 86, issue 3, page 577-589 ISSN 0025-3154 1469-7769 journal-article 2006 crcambridgeupr https://doi.org/10.1017/s002531540601349x 2024-07-03T04:03:34Z This study examined the relationship between viral infection and the richness, diversity and composition of bacterial assemblages in the water column. Viruses were enriched by ultrafiltration, added to water column incubation experiments at 15 locations in the North Atlantic, North Pacific, Gulf of Mexico and Southern California. In a separate experiment, viruses were removed from bacterioplankton by diafiltration at the San Pedro Ocean Time Series Station. Bacterial assemblage composition was observed using a high throughput and sensitive molecular fingerprinting analysis, automated rRNA intergenic spacer analysis (ARISA). Diazotrophs were used as a model functional group to represent rare organisms hypothesized to benefit from viral activity, and their richness and diversity was determined by terminal restriction fragment length polymorphism of a nitrogenase gene fragment ( nifH ). The enrichment and removal experiments demonstrated mixed impacts of viral pressure upon bacterial communities, and we observed significant effects of viruses on several microbial parameters in all but two experiments. However, there was no consistent response of viral enrichment on total bacterial and diazotroph assemblages at stations with similar environmental conditions, suggesting that untested variables, small spatial scale factors, or stochastic processes influence the outcome of viral activities. Across all experiments, the relative abundance of the more common operational taxonomic units (OTUs) in fingerprints were not significantly impacted compared to the abundance of rare OTUs. These data indicate that viruses may have significant influence upon community structure of bacterioplankton; however, effects were not consistent between sampling locations nor water masses. Article in Journal/Newspaper North Atlantic Cambridge University Press Journal of the Marine Biological Association of the United Kingdom 86 3 577 589 |
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Open Polar |
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Cambridge University Press |
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crcambridgeupr |
language |
English |
description |
This study examined the relationship between viral infection and the richness, diversity and composition of bacterial assemblages in the water column. Viruses were enriched by ultrafiltration, added to water column incubation experiments at 15 locations in the North Atlantic, North Pacific, Gulf of Mexico and Southern California. In a separate experiment, viruses were removed from bacterioplankton by diafiltration at the San Pedro Ocean Time Series Station. Bacterial assemblage composition was observed using a high throughput and sensitive molecular fingerprinting analysis, automated rRNA intergenic spacer analysis (ARISA). Diazotrophs were used as a model functional group to represent rare organisms hypothesized to benefit from viral activity, and their richness and diversity was determined by terminal restriction fragment length polymorphism of a nitrogenase gene fragment ( nifH ). The enrichment and removal experiments demonstrated mixed impacts of viral pressure upon bacterial communities, and we observed significant effects of viruses on several microbial parameters in all but two experiments. However, there was no consistent response of viral enrichment on total bacterial and diazotroph assemblages at stations with similar environmental conditions, suggesting that untested variables, small spatial scale factors, or stochastic processes influence the outcome of viral activities. Across all experiments, the relative abundance of the more common operational taxonomic units (OTUs) in fingerprints were not significantly impacted compared to the abundance of rare OTUs. These data indicate that viruses may have significant influence upon community structure of bacterioplankton; however, effects were not consistent between sampling locations nor water masses. |
format |
Article in Journal/Newspaper |
author |
Hewson, Ian Fuhrman, Jed A. |
spellingShingle |
Hewson, Ian Fuhrman, Jed A. Viral impacts upon marine bacterioplankton assemblage structure |
author_facet |
Hewson, Ian Fuhrman, Jed A. |
author_sort |
Hewson, Ian |
title |
Viral impacts upon marine bacterioplankton assemblage structure |
title_short |
Viral impacts upon marine bacterioplankton assemblage structure |
title_full |
Viral impacts upon marine bacterioplankton assemblage structure |
title_fullStr |
Viral impacts upon marine bacterioplankton assemblage structure |
title_full_unstemmed |
Viral impacts upon marine bacterioplankton assemblage structure |
title_sort |
viral impacts upon marine bacterioplankton assemblage structure |
publisher |
Cambridge University Press (CUP) |
publishDate |
2006 |
url |
http://dx.doi.org/10.1017/s002531540601349x https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S002531540601349X |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
Journal of the Marine Biological Association of the United Kingdom volume 86, issue 3, page 577-589 ISSN 0025-3154 1469-7769 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s002531540601349x |
container_title |
Journal of the Marine Biological Association of the United Kingdom |
container_volume |
86 |
container_issue |
3 |
container_start_page |
577 |
op_container_end_page |
589 |
_version_ |
1810463998217289728 |