On the nutrition and metabolism of zooplankton XII. Measurements by radioimmunoassay of the levels of a steroid in Calanus

• Published in: Journal of the Marine Biological Association of the United Kingdom
• Main Authors: O'Hara, S. C. M., Corner, E. D. S., Kilvington, C. C.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Cambridge University Press (CUP) 1978
• Subjects: Aquatic Science; Norway; Calanus finmarchicus
• Online Access: http://dx.doi.org/10.1017/s0025315400041254; https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0025315400041254

A steroid that binds with mammalian oestradiol-17β antibody has been detected in Calanus helgolandicus Claus, collected from Plymouth Sound, and in Calanus finmarchicus Gunnerus captured off the coast of Norway. The specificity of the antibody, together with the chromatographic behaviour of the steroid on Sephadex LH-20, supports the view that the compound is the sex hormone oestradiol-17β (E2). The antibody has been used in measuring the amounts of E2 in the animals by radioimmunoassay, a technique not previously employed in studies with zooplankton and therefore described in detail.