Uptake, tissue distribution and metabolism of 14 C-labelled tributyltin (TBT) in the dog-whelk, Nucella lapillus
A study of tributyltin (TBT) uptake, tissue distribution and breakdown in dog-whelks, Nucella lapillus , was carried out using 14 C-labelled tributyltin chloride. The compound was introduced into animals by exposure to sea-water, from a diet of labelled mussels and by injection. Measurements of 14 C...
Published in: | Journal of the Marine Biological Association of the United Kingdom |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Cambridge University Press (CUP)
1993
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1017/s0025315400034792 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0025315400034792 |
id |
crcambridgeupr:10.1017/s0025315400034792 |
---|---|
record_format |
openpolar |
spelling |
crcambridgeupr:10.1017/s0025315400034792 2024-09-30T14:46:20+00:00 Uptake, tissue distribution and metabolism of 14 C-labelled tributyltin (TBT) in the dog-whelk, Nucella lapillus Bryan, G. W. Bright, D. A. Hummerstone, L. G. Burt, G. R. 1993 http://dx.doi.org/10.1017/s0025315400034792 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0025315400034792 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Journal of the Marine Biological Association of the United Kingdom volume 73, issue 4, page 889-912 ISSN 0025-3154 1469-7769 journal-article 1993 crcambridgeupr https://doi.org/10.1017/s0025315400034792 2024-09-04T04:03:47Z A study of tributyltin (TBT) uptake, tissue distribution and breakdown in dog-whelks, Nucella lapillus , was carried out using 14 C-labelled tributyltin chloride. The compound was introduced into animals by exposure to sea-water, from a diet of labelled mussels and by injection. Measurements of 14 C were made on tissue extracts containing total 14 C, TBT+DBT and TBT. Organs examined included digestive gland, kidney, mantle, foot, viscera, gonads and blood. Absorption of dissolved TBT occurred primarily via tissues associated with the mantle: autoradiographic evidence showed that of these the ctenidium (gill) and osphradium were probably most important. Of non-reproductive tissues, mantle displayed least evidence of TBT degradation whereas kidney and digestive gland contained lower proportions of 14 C as TBT (~60%) than other tissues. By virtue of its more rapid response, initially at least, the kidney had a primary role in degrading dissolved TBT that reached the blood. Dog-whelks feeding on labelled Mytilus edulis absorbed organotins very efficiently via the digestive gland, which then played a major role in TBT breakdown. However, the capacity for degradation is not sufficient to prevent, TBT concentration factors of the order of 100,000 (dry tissue/water) being attained in N. lapillus . Autoradiography revealed surprisingly high grain-counts in nerves, which may be significant in view of evidence in the literature that neurohormones are intermediaries in the chain linking TBT with imposex. Article in Journal/Newspaper Dog whelk Nucella lapillus Cambridge University Press Journal of the Marine Biological Association of the United Kingdom 73 4 889 912 |
institution |
Open Polar |
collection |
Cambridge University Press |
op_collection_id |
crcambridgeupr |
language |
English |
description |
A study of tributyltin (TBT) uptake, tissue distribution and breakdown in dog-whelks, Nucella lapillus , was carried out using 14 C-labelled tributyltin chloride. The compound was introduced into animals by exposure to sea-water, from a diet of labelled mussels and by injection. Measurements of 14 C were made on tissue extracts containing total 14 C, TBT+DBT and TBT. Organs examined included digestive gland, kidney, mantle, foot, viscera, gonads and blood. Absorption of dissolved TBT occurred primarily via tissues associated with the mantle: autoradiographic evidence showed that of these the ctenidium (gill) and osphradium were probably most important. Of non-reproductive tissues, mantle displayed least evidence of TBT degradation whereas kidney and digestive gland contained lower proportions of 14 C as TBT (~60%) than other tissues. By virtue of its more rapid response, initially at least, the kidney had a primary role in degrading dissolved TBT that reached the blood. Dog-whelks feeding on labelled Mytilus edulis absorbed organotins very efficiently via the digestive gland, which then played a major role in TBT breakdown. However, the capacity for degradation is not sufficient to prevent, TBT concentration factors of the order of 100,000 (dry tissue/water) being attained in N. lapillus . Autoradiography revealed surprisingly high grain-counts in nerves, which may be significant in view of evidence in the literature that neurohormones are intermediaries in the chain linking TBT with imposex. |
format |
Article in Journal/Newspaper |
author |
Bryan, G. W. Bright, D. A. Hummerstone, L. G. Burt, G. R. |
spellingShingle |
Bryan, G. W. Bright, D. A. Hummerstone, L. G. Burt, G. R. Uptake, tissue distribution and metabolism of 14 C-labelled tributyltin (TBT) in the dog-whelk, Nucella lapillus |
author_facet |
Bryan, G. W. Bright, D. A. Hummerstone, L. G. Burt, G. R. |
author_sort |
Bryan, G. W. |
title |
Uptake, tissue distribution and metabolism of 14 C-labelled tributyltin (TBT) in the dog-whelk, Nucella lapillus |
title_short |
Uptake, tissue distribution and metabolism of 14 C-labelled tributyltin (TBT) in the dog-whelk, Nucella lapillus |
title_full |
Uptake, tissue distribution and metabolism of 14 C-labelled tributyltin (TBT) in the dog-whelk, Nucella lapillus |
title_fullStr |
Uptake, tissue distribution and metabolism of 14 C-labelled tributyltin (TBT) in the dog-whelk, Nucella lapillus |
title_full_unstemmed |
Uptake, tissue distribution and metabolism of 14 C-labelled tributyltin (TBT) in the dog-whelk, Nucella lapillus |
title_sort |
uptake, tissue distribution and metabolism of 14 c-labelled tributyltin (tbt) in the dog-whelk, nucella lapillus |
publisher |
Cambridge University Press (CUP) |
publishDate |
1993 |
url |
http://dx.doi.org/10.1017/s0025315400034792 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0025315400034792 |
genre |
Dog whelk Nucella lapillus |
genre_facet |
Dog whelk Nucella lapillus |
op_source |
Journal of the Marine Biological Association of the United Kingdom volume 73, issue 4, page 889-912 ISSN 0025-3154 1469-7769 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0025315400034792 |
container_title |
Journal of the Marine Biological Association of the United Kingdom |
container_volume |
73 |
container_issue |
4 |
container_start_page |
889 |
op_container_end_page |
912 |
_version_ |
1811646398162010112 |