Natural Bactericidal Antibodies: Observations on the Bactericidal Mechanism of Normal Serum

1. An analytical study has been made of the mechanism of natural bactericidal action by the serum of various animals (ox, sheep, horse, rabbit, guinea-pig, rat, man) towards certain organisms ( B. typhosus, B. dysenteriae Shiga, B. proteus, V. cholerae ) exhibiting the maximum reactivity to this eff...

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Published in:Journal of Hygiene
Main Authors: Mackie, T. J., Finkelstein, M. H.
Format: Article in Journal/Newspaper
Language:English
Published: Cambridge University Press (CUP) 1931
Subjects:
Online Access:http://dx.doi.org/10.1017/s0022172400010676
https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0022172400010676
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spelling crcambridgeupr:10.1017/s0022172400010676 2024-03-03T08:43:34+00:00 Natural Bactericidal Antibodies: Observations on the Bactericidal Mechanism of Normal Serum Mackie, T. J. Finkelstein, M. H. 1931 http://dx.doi.org/10.1017/s0022172400010676 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0022172400010676 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Journal of Hygiene volume 31, issue 1, page 35-55 ISSN 0022-1724 Public Health, Environmental and Occupational Health Immunology journal-article 1931 crcambridgeupr https://doi.org/10.1017/s0022172400010676 2024-02-08T08:30:58Z 1. An analytical study has been made of the mechanism of natural bactericidal action by the serum of various animals (ox, sheep, horse, rabbit, guinea-pig, rat, man) towards certain organisms ( B. typhosus, B. dysenteriae Shiga, B. proteus, V. cholerae ) exhibiting the maximum reactivity to this effect. 2. Serum-complement has no bactericidal action per se , and an antibodylike agent invariably acts as an intermediary agent, “sensitising” the particular organism to the action of the complement and capable of being “absorbed” by it from serum at 0° C. 3. This sensitising agent is stable at 55° C. but labile at 60°–65° C. In this respect it resembles natural haemolysins and agglutinins, but contrasts with the more stable immune antibodies and the more labile natural complement-fixing antibodies (for bacterial antigens). It is resident mainly in the carbonic-acid-insoluble fraction of the serum. It is present in the serum of young animals before certain other natural antibodies have developed. 4. Absorption tests demonstrate the high degree of specificity of these natural bactericidal antibodies for particular bacteria. 5. A non-specific extracellular substance occurs in bacterial cultures which may neutralise or inhibit these antibodies, and interfere with their sensitising action even at 0° C. 6. This substance is liberated in large amount in cultures heated at high temperatures (120° C). It can be removed by repeated washing of growths in saline solution. It may inactivate a bactericidal antibody in heated serum, though not in fresh unheated serum, and may inactivate a particular antibody in the serum of one animal species but not in another. Strains of bacteria vary in their production of this substance. 7. The observations submitted in this paper, correlated with previous studies of natural antibodies by the authors and others, indicate that immune antibodies have their precursors specifically differentiated in the serum of normal animals and that, in general, immune antibodies are not substances formed de ... Article in Journal/Newspaper Carbonic acid Cambridge University Press Journal of Hygiene 31 1 35 55
institution Open Polar
collection Cambridge University Press
op_collection_id crcambridgeupr
language English
topic Public Health, Environmental and Occupational Health
Immunology
spellingShingle Public Health, Environmental and Occupational Health
Immunology
Mackie, T. J.
Finkelstein, M. H.
Natural Bactericidal Antibodies: Observations on the Bactericidal Mechanism of Normal Serum
topic_facet Public Health, Environmental and Occupational Health
Immunology
description 1. An analytical study has been made of the mechanism of natural bactericidal action by the serum of various animals (ox, sheep, horse, rabbit, guinea-pig, rat, man) towards certain organisms ( B. typhosus, B. dysenteriae Shiga, B. proteus, V. cholerae ) exhibiting the maximum reactivity to this effect. 2. Serum-complement has no bactericidal action per se , and an antibodylike agent invariably acts as an intermediary agent, “sensitising” the particular organism to the action of the complement and capable of being “absorbed” by it from serum at 0° C. 3. This sensitising agent is stable at 55° C. but labile at 60°–65° C. In this respect it resembles natural haemolysins and agglutinins, but contrasts with the more stable immune antibodies and the more labile natural complement-fixing antibodies (for bacterial antigens). It is resident mainly in the carbonic-acid-insoluble fraction of the serum. It is present in the serum of young animals before certain other natural antibodies have developed. 4. Absorption tests demonstrate the high degree of specificity of these natural bactericidal antibodies for particular bacteria. 5. A non-specific extracellular substance occurs in bacterial cultures which may neutralise or inhibit these antibodies, and interfere with their sensitising action even at 0° C. 6. This substance is liberated in large amount in cultures heated at high temperatures (120° C). It can be removed by repeated washing of growths in saline solution. It may inactivate a bactericidal antibody in heated serum, though not in fresh unheated serum, and may inactivate a particular antibody in the serum of one animal species but not in another. Strains of bacteria vary in their production of this substance. 7. The observations submitted in this paper, correlated with previous studies of natural antibodies by the authors and others, indicate that immune antibodies have their precursors specifically differentiated in the serum of normal animals and that, in general, immune antibodies are not substances formed de ...
format Article in Journal/Newspaper
author Mackie, T. J.
Finkelstein, M. H.
author_facet Mackie, T. J.
Finkelstein, M. H.
author_sort Mackie, T. J.
title Natural Bactericidal Antibodies: Observations on the Bactericidal Mechanism of Normal Serum
title_short Natural Bactericidal Antibodies: Observations on the Bactericidal Mechanism of Normal Serum
title_full Natural Bactericidal Antibodies: Observations on the Bactericidal Mechanism of Normal Serum
title_fullStr Natural Bactericidal Antibodies: Observations on the Bactericidal Mechanism of Normal Serum
title_full_unstemmed Natural Bactericidal Antibodies: Observations on the Bactericidal Mechanism of Normal Serum
title_sort natural bactericidal antibodies: observations on the bactericidal mechanism of normal serum
publisher Cambridge University Press (CUP)
publishDate 1931
url http://dx.doi.org/10.1017/s0022172400010676
https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0022172400010676
genre Carbonic acid
genre_facet Carbonic acid
op_source Journal of Hygiene
volume 31, issue 1, page 35-55
ISSN 0022-1724
op_rights https://www.cambridge.org/core/terms
op_doi https://doi.org/10.1017/s0022172400010676
container_title Journal of Hygiene
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container_issue 1
container_start_page 35
op_container_end_page 55
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