A report on the complete mitochondrial genome of the trematode Azygia robusta Odhner, 1911, its new definitive host from the Russian Far East, and unexpected phylogeny of Azygiidae within Digenea, as inferred from mitogenome sequences
Abstract New data on the complete mitochondrial genome of Azygia robusta (Azygiidae) were obtained by the next-generation sequencing (NGS) approach. The mitochondrial DNA (mtDNA) of A. robusta had a length of 13 857 bp and included 12 protein-coding genes, two ribosomal genes, 22 transfer RNA genes,...
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crcambridgeupr:10.1017/s0022149x23000500 2024-03-03T08:48:32+00:00 A report on the complete mitochondrial genome of the trematode Azygia robusta Odhner, 1911, its new definitive host from the Russian Far East, and unexpected phylogeny of Azygiidae within Digenea, as inferred from mitogenome sequences Atopkin, D. M. Semenchenko, A. A. Solodovnik, D. A. Ivashko, Y. I. 2023 http://dx.doi.org/10.1017/s0022149x23000500 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0022149X23000500 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Journal of Helminthology volume 97 ISSN 0022-149X 1475-2697 Animal Science and Zoology General Medicine Parasitology journal-article 2023 crcambridgeupr https://doi.org/10.1017/s0022149x23000500 2024-02-08T08:48:59Z Abstract New data on the complete mitochondrial genome of Azygia robusta (Azygiidae) were obtained by the next-generation sequencing (NGS) approach. The mitochondrial DNA (mtDNA) of A. robusta had a length of 13 857 bp and included 12 protein-coding genes, two ribosomal genes, 22 transfer RNA genes, and two non-coding regions. The nucleotide sequences of the complete mitochondrial genomes of two A. robusta specimens differed from each other by 0.12 ± 0.03%. Six of 12 protein-coding genes demonstrated intraspecific variation. The difference between the nucleotide sequences of the complete mitochondrial genomes of A. robusta and Azygia hwangtsiyui was 26.95 ± 0.35%; the interspecific variation of protein-coding genes between A. robusta and A. hwangtsiyui ranged from 20.5 ± 0.9% ( cox 1) to 30.7 ± 1.2% ( nad 5). The observed gene arrangement in the mtDNA sequence of A. robusta was identical to that of A. hwangtsiyui. Codon usage and amino acid frequencies were highly similar between A. robusta and A. hwangtsiyui. The results of phylogenetic analyses based on mtDNA protein-coding regions showed that A. robusta is closely related to A. hwangtsiyui (belonging to the same suborder, Azygiida) that formed a distinct early-diverging branch relative to all other Digenea. A preliminary morphological analysis of paratypes of the two azygiid specimens studied showed visible morphological differences between them. The specimen extracted from Sakhalin taimen ( Parahucho perryi ) was most similar to A. robusta. Thus, we here provide the first record of a new definitive host, P. perryi , for A. robusta and also molecular characteristics of the trematode specimens. Article in Journal/Newspaper Sakhalin Cambridge University Press Journal of Helminthology 97 |
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Cambridge University Press |
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crcambridgeupr |
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English |
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Animal Science and Zoology General Medicine Parasitology |
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Animal Science and Zoology General Medicine Parasitology Atopkin, D. M. Semenchenko, A. A. Solodovnik, D. A. Ivashko, Y. I. A report on the complete mitochondrial genome of the trematode Azygia robusta Odhner, 1911, its new definitive host from the Russian Far East, and unexpected phylogeny of Azygiidae within Digenea, as inferred from mitogenome sequences |
topic_facet |
Animal Science and Zoology General Medicine Parasitology |
description |
Abstract New data on the complete mitochondrial genome of Azygia robusta (Azygiidae) were obtained by the next-generation sequencing (NGS) approach. The mitochondrial DNA (mtDNA) of A. robusta had a length of 13 857 bp and included 12 protein-coding genes, two ribosomal genes, 22 transfer RNA genes, and two non-coding regions. The nucleotide sequences of the complete mitochondrial genomes of two A. robusta specimens differed from each other by 0.12 ± 0.03%. Six of 12 protein-coding genes demonstrated intraspecific variation. The difference between the nucleotide sequences of the complete mitochondrial genomes of A. robusta and Azygia hwangtsiyui was 26.95 ± 0.35%; the interspecific variation of protein-coding genes between A. robusta and A. hwangtsiyui ranged from 20.5 ± 0.9% ( cox 1) to 30.7 ± 1.2% ( nad 5). The observed gene arrangement in the mtDNA sequence of A. robusta was identical to that of A. hwangtsiyui. Codon usage and amino acid frequencies were highly similar between A. robusta and A. hwangtsiyui. The results of phylogenetic analyses based on mtDNA protein-coding regions showed that A. robusta is closely related to A. hwangtsiyui (belonging to the same suborder, Azygiida) that formed a distinct early-diverging branch relative to all other Digenea. A preliminary morphological analysis of paratypes of the two azygiid specimens studied showed visible morphological differences between them. The specimen extracted from Sakhalin taimen ( Parahucho perryi ) was most similar to A. robusta. Thus, we here provide the first record of a new definitive host, P. perryi , for A. robusta and also molecular characteristics of the trematode specimens. |
format |
Article in Journal/Newspaper |
author |
Atopkin, D. M. Semenchenko, A. A. Solodovnik, D. A. Ivashko, Y. I. |
author_facet |
Atopkin, D. M. Semenchenko, A. A. Solodovnik, D. A. Ivashko, Y. I. |
author_sort |
Atopkin, D. M. |
title |
A report on the complete mitochondrial genome of the trematode Azygia robusta Odhner, 1911, its new definitive host from the Russian Far East, and unexpected phylogeny of Azygiidae within Digenea, as inferred from mitogenome sequences |
title_short |
A report on the complete mitochondrial genome of the trematode Azygia robusta Odhner, 1911, its new definitive host from the Russian Far East, and unexpected phylogeny of Azygiidae within Digenea, as inferred from mitogenome sequences |
title_full |
A report on the complete mitochondrial genome of the trematode Azygia robusta Odhner, 1911, its new definitive host from the Russian Far East, and unexpected phylogeny of Azygiidae within Digenea, as inferred from mitogenome sequences |
title_fullStr |
A report on the complete mitochondrial genome of the trematode Azygia robusta Odhner, 1911, its new definitive host from the Russian Far East, and unexpected phylogeny of Azygiidae within Digenea, as inferred from mitogenome sequences |
title_full_unstemmed |
A report on the complete mitochondrial genome of the trematode Azygia robusta Odhner, 1911, its new definitive host from the Russian Far East, and unexpected phylogeny of Azygiidae within Digenea, as inferred from mitogenome sequences |
title_sort |
report on the complete mitochondrial genome of the trematode azygia robusta odhner, 1911, its new definitive host from the russian far east, and unexpected phylogeny of azygiidae within digenea, as inferred from mitogenome sequences |
publisher |
Cambridge University Press (CUP) |
publishDate |
2023 |
url |
http://dx.doi.org/10.1017/s0022149x23000500 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0022149X23000500 |
genre |
Sakhalin |
genre_facet |
Sakhalin |
op_source |
Journal of Helminthology volume 97 ISSN 0022-149X 1475-2697 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0022149x23000500 |
container_title |
Journal of Helminthology |
container_volume |
97 |
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1792505433548652544 |