Morphological, molecular and phylogenetic analyses of the spirurid nematode Stegophorus macronectes (Johnston & Mawson, 1942)
Abstract Stegophorus macronectes (Johnston & Mawson, 1942) is a gastrointestinal parasite found in Antarctic seabirds. The original description of the species, which was based only on females, is poor and fragmented with some unclear diagnostic characters. This study provides new morphometric an...
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Online Access: | http://dx.doi.org/10.1017/s0022149x15000218 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0022149X15000218 |
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crcambridgeupr:10.1017/s0022149x15000218 2024-03-03T08:38:14+00:00 Morphological, molecular and phylogenetic analyses of the spirurid nematode Stegophorus macronectes (Johnston & Mawson, 1942) Vidal, V. Ortiz, J. Diaz, J.I. Zafrilla, B. Bonete, M.J. Ruiz De Ybañez, M.R. Palacios, M.J. Benzal, J. Valera, F. De La Cruz, C. Motas, M. Bautista, V. Machordom, A. Barbosa, A. 2015 http://dx.doi.org/10.1017/s0022149x15000218 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0022149X15000218 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Journal of Helminthology volume 90, issue 2, page 214-222 ISSN 0022-149X 1475-2697 Animal Science and Zoology General Medicine Parasitology journal-article 2015 crcambridgeupr https://doi.org/10.1017/s0022149x15000218 2024-02-08T08:33:05Z Abstract Stegophorus macronectes (Johnston & Mawson, 1942) is a gastrointestinal parasite found in Antarctic seabirds. The original description of the species, which was based only on females, is poor and fragmented with some unclear diagnostic characters. This study provides new morphometric and molecular data on this previously poorly described parasite. Nuclear rDNA sequences (18S, 5.8S, 28S and internal transcribed spacer (ITS) regions) were isolated from S. macronectes specimens collected from the chinstrap penguin Pygoscelis antarctica Forster on Deception Island, Antarctica. Using 18S rDNA sequences, phylogenetic analyses (maximum likelihood, maximum parsimony and Bayesian inference) of the order Spirurida were performed to determine the phylogenetic location of this species. Primer pairs of the ITS regions were designed for genus-level identification of specimens, regardless of their cycle, as an alternative to coprological methods. The utility of this molecular method for identification of morphologically altered specimens is also discussed. Article in Journal/Newspaper Antarc* Antarctic Antarctica Chinstrap penguin Deception Island Pygoscelis antarctica Cambridge University Press Antarctic Deception Island ENVELOPE(-60.633,-60.633,-62.950,-62.950) Journal of Helminthology 90 2 214 222 |
institution |
Open Polar |
collection |
Cambridge University Press |
op_collection_id |
crcambridgeupr |
language |
English |
topic |
Animal Science and Zoology General Medicine Parasitology |
spellingShingle |
Animal Science and Zoology General Medicine Parasitology Vidal, V. Ortiz, J. Diaz, J.I. Zafrilla, B. Bonete, M.J. Ruiz De Ybañez, M.R. Palacios, M.J. Benzal, J. Valera, F. De La Cruz, C. Motas, M. Bautista, V. Machordom, A. Barbosa, A. Morphological, molecular and phylogenetic analyses of the spirurid nematode Stegophorus macronectes (Johnston & Mawson, 1942) |
topic_facet |
Animal Science and Zoology General Medicine Parasitology |
description |
Abstract Stegophorus macronectes (Johnston & Mawson, 1942) is a gastrointestinal parasite found in Antarctic seabirds. The original description of the species, which was based only on females, is poor and fragmented with some unclear diagnostic characters. This study provides new morphometric and molecular data on this previously poorly described parasite. Nuclear rDNA sequences (18S, 5.8S, 28S and internal transcribed spacer (ITS) regions) were isolated from S. macronectes specimens collected from the chinstrap penguin Pygoscelis antarctica Forster on Deception Island, Antarctica. Using 18S rDNA sequences, phylogenetic analyses (maximum likelihood, maximum parsimony and Bayesian inference) of the order Spirurida were performed to determine the phylogenetic location of this species. Primer pairs of the ITS regions were designed for genus-level identification of specimens, regardless of their cycle, as an alternative to coprological methods. The utility of this molecular method for identification of morphologically altered specimens is also discussed. |
format |
Article in Journal/Newspaper |
author |
Vidal, V. Ortiz, J. Diaz, J.I. Zafrilla, B. Bonete, M.J. Ruiz De Ybañez, M.R. Palacios, M.J. Benzal, J. Valera, F. De La Cruz, C. Motas, M. Bautista, V. Machordom, A. Barbosa, A. |
author_facet |
Vidal, V. Ortiz, J. Diaz, J.I. Zafrilla, B. Bonete, M.J. Ruiz De Ybañez, M.R. Palacios, M.J. Benzal, J. Valera, F. De La Cruz, C. Motas, M. Bautista, V. Machordom, A. Barbosa, A. |
author_sort |
Vidal, V. |
title |
Morphological, molecular and phylogenetic analyses of the spirurid nematode Stegophorus macronectes (Johnston & Mawson, 1942) |
title_short |
Morphological, molecular and phylogenetic analyses of the spirurid nematode Stegophorus macronectes (Johnston & Mawson, 1942) |
title_full |
Morphological, molecular and phylogenetic analyses of the spirurid nematode Stegophorus macronectes (Johnston & Mawson, 1942) |
title_fullStr |
Morphological, molecular and phylogenetic analyses of the spirurid nematode Stegophorus macronectes (Johnston & Mawson, 1942) |
title_full_unstemmed |
Morphological, molecular and phylogenetic analyses of the spirurid nematode Stegophorus macronectes (Johnston & Mawson, 1942) |
title_sort |
morphological, molecular and phylogenetic analyses of the spirurid nematode stegophorus macronectes (johnston & mawson, 1942) |
publisher |
Cambridge University Press (CUP) |
publishDate |
2015 |
url |
http://dx.doi.org/10.1017/s0022149x15000218 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0022149X15000218 |
long_lat |
ENVELOPE(-60.633,-60.633,-62.950,-62.950) |
geographic |
Antarctic Deception Island |
geographic_facet |
Antarctic Deception Island |
genre |
Antarc* Antarctic Antarctica Chinstrap penguin Deception Island Pygoscelis antarctica |
genre_facet |
Antarc* Antarctic Antarctica Chinstrap penguin Deception Island Pygoscelis antarctica |
op_source |
Journal of Helminthology volume 90, issue 2, page 214-222 ISSN 0022-149X 1475-2697 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0022149x15000218 |
container_title |
Journal of Helminthology |
container_volume |
90 |
container_issue |
2 |
container_start_page |
214 |
op_container_end_page |
222 |
_version_ |
1792505808379969536 |