Morphological and molecular analyses of larval and adult stages of Echinoparyphium recurvatum von Linstow 1873 (Digenea: Echinostomatidae) from central Mexico
Abstract In central Mexico, populations of the freshwater snail Physella cubensis were infected with metacercariae from a species of Echinoparyphium (Digenea: Echinostomatidae). In the current study, we describe both larval and adult stages of this species obtained from experimental and natural infe...
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crcambridgeupr:10.1017/s0022149x14000297 2024-03-03T08:36:39+00:00 Morphological and molecular analyses of larval and adult stages of Echinoparyphium recurvatum von Linstow 1873 (Digenea: Echinostomatidae) from central Mexico Sereno-Uribe, A.L. Pinacho-Pinacho, C.D. Sanchéz Cordero, V. García-Varela, M. 2014 http://dx.doi.org/10.1017/s0022149x14000297 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0022149X14000297 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Journal of Helminthology volume 89, issue 4, page 458-464 ISSN 0022-149X 1475-2697 Animal Science and Zoology General Medicine Parasitology journal-article 2014 crcambridgeupr https://doi.org/10.1017/s0022149x14000297 2024-02-08T08:26:31Z Abstract In central Mexico, populations of the freshwater snail Physella cubensis were infected with metacercariae from a species of Echinoparyphium (Digenea: Echinostomatidae). In the current study, we describe both larval and adult stages of this species obtained from experimental and natural infections. A total 180 snails were collected from Patzcuaro Lake, Michoacan state in central Mexico in July 2012. In the laboratory snails were placed in individual vials and exposed to light with the aim of observing emergence of cercariae. To obtain metacercariae, uninfected snails ( P. cubensis ) were exposed to cercariae. Chicks were infected with metacercariae to obtain adults. Nine days post-infection, eggs were recovered and incubated in tap water at room temperature to observe miracidia. Adults obtained from natural and experimentally infected hosts possess a head collar with 45 spines in two alternating rows, confirming the identification as Echinoparyphium recurvatum von Linstow 1873. To test the conspecificity of all stages, sequences of nuclear internal transcribed spacer 1 (ITS1), 5.8S and ITS2 rDNA were obtained from two adult worms recovered from chicks and also a natural avian host, the shoveler duck Anas clypeata , together with five cercarial and four metacercarial isolates from nine snails. The genetic divergence estimated among the 13 isolates was very low, ranging from 0 to 0.6%. Phylogenetic analyses inferred by maximum likelihood and Bayesian methods showed that all isolates of E. recurvatum form a single clade with strong support. The presence of E. recurvatum in P. cubensis and A. clypeata from central Mexico represents new host reports, and extends the distribution range in the Americas. Article in Journal/Newspaper Anas clypeata Shoveler Cambridge University Press Journal of Helminthology 89 4 458 464 |
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Cambridge University Press |
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English |
topic |
Animal Science and Zoology General Medicine Parasitology |
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Animal Science and Zoology General Medicine Parasitology Sereno-Uribe, A.L. Pinacho-Pinacho, C.D. Sanchéz Cordero, V. García-Varela, M. Morphological and molecular analyses of larval and adult stages of Echinoparyphium recurvatum von Linstow 1873 (Digenea: Echinostomatidae) from central Mexico |
topic_facet |
Animal Science and Zoology General Medicine Parasitology |
description |
Abstract In central Mexico, populations of the freshwater snail Physella cubensis were infected with metacercariae from a species of Echinoparyphium (Digenea: Echinostomatidae). In the current study, we describe both larval and adult stages of this species obtained from experimental and natural infections. A total 180 snails were collected from Patzcuaro Lake, Michoacan state in central Mexico in July 2012. In the laboratory snails were placed in individual vials and exposed to light with the aim of observing emergence of cercariae. To obtain metacercariae, uninfected snails ( P. cubensis ) were exposed to cercariae. Chicks were infected with metacercariae to obtain adults. Nine days post-infection, eggs were recovered and incubated in tap water at room temperature to observe miracidia. Adults obtained from natural and experimentally infected hosts possess a head collar with 45 spines in two alternating rows, confirming the identification as Echinoparyphium recurvatum von Linstow 1873. To test the conspecificity of all stages, sequences of nuclear internal transcribed spacer 1 (ITS1), 5.8S and ITS2 rDNA were obtained from two adult worms recovered from chicks and also a natural avian host, the shoveler duck Anas clypeata , together with five cercarial and four metacercarial isolates from nine snails. The genetic divergence estimated among the 13 isolates was very low, ranging from 0 to 0.6%. Phylogenetic analyses inferred by maximum likelihood and Bayesian methods showed that all isolates of E. recurvatum form a single clade with strong support. The presence of E. recurvatum in P. cubensis and A. clypeata from central Mexico represents new host reports, and extends the distribution range in the Americas. |
format |
Article in Journal/Newspaper |
author |
Sereno-Uribe, A.L. Pinacho-Pinacho, C.D. Sanchéz Cordero, V. García-Varela, M. |
author_facet |
Sereno-Uribe, A.L. Pinacho-Pinacho, C.D. Sanchéz Cordero, V. García-Varela, M. |
author_sort |
Sereno-Uribe, A.L. |
title |
Morphological and molecular analyses of larval and adult stages of Echinoparyphium recurvatum von Linstow 1873 (Digenea: Echinostomatidae) from central Mexico |
title_short |
Morphological and molecular analyses of larval and adult stages of Echinoparyphium recurvatum von Linstow 1873 (Digenea: Echinostomatidae) from central Mexico |
title_full |
Morphological and molecular analyses of larval and adult stages of Echinoparyphium recurvatum von Linstow 1873 (Digenea: Echinostomatidae) from central Mexico |
title_fullStr |
Morphological and molecular analyses of larval and adult stages of Echinoparyphium recurvatum von Linstow 1873 (Digenea: Echinostomatidae) from central Mexico |
title_full_unstemmed |
Morphological and molecular analyses of larval and adult stages of Echinoparyphium recurvatum von Linstow 1873 (Digenea: Echinostomatidae) from central Mexico |
title_sort |
morphological and molecular analyses of larval and adult stages of echinoparyphium recurvatum von linstow 1873 (digenea: echinostomatidae) from central mexico |
publisher |
Cambridge University Press (CUP) |
publishDate |
2014 |
url |
http://dx.doi.org/10.1017/s0022149x14000297 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0022149X14000297 |
genre |
Anas clypeata Shoveler |
genre_facet |
Anas clypeata Shoveler |
op_source |
Journal of Helminthology volume 89, issue 4, page 458-464 ISSN 0022-149X 1475-2697 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0022149x14000297 |
container_title |
Journal of Helminthology |
container_volume |
89 |
container_issue |
4 |
container_start_page |
458 |
op_container_end_page |
464 |
_version_ |
1792494573264568320 |