Selected mitochondrial genes as species markers of the Arctic Contracaecum osculatumcomplex
Abstract This study, aimed at testing the hypothesis that some mitochondrial genes can serve as species-specific markers, involved a comparison of the sequence variance of selected mitochondrial DNA genes of the Arctic Contracaecum osculatum species ( C. osculatum A, C. osculatum B and C. osculatum...
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Online Access: | http://dx.doi.org/10.1017/s0022149x11000332 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0022149X11000332 |
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crcambridgeupr:10.1017/s0022149x11000332 2024-06-23T07:50:00+00:00 Selected mitochondrial genes as species markers of the Arctic Contracaecum osculatumcomplex Dzido, J. Kijewska, A. Rokicki, J. 2011 http://dx.doi.org/10.1017/s0022149x11000332 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0022149X11000332 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Journal of Helminthology volume 86, issue 2, page 252-258 ISSN 0022-149X 1475-2697 journal-article 2011 crcambridgeupr https://doi.org/10.1017/s0022149x11000332 2024-06-12T04:04:03Z Abstract This study, aimed at testing the hypothesis that some mitochondrial genes can serve as species-specific markers, involved a comparison of the sequence variance of selected mitochondrial DNA genes of the Arctic Contracaecum osculatum species ( C. osculatum A, C. osculatum B and C. osculatum C). We compared differences between five complete (ND2, CYTB, ND3, ND4L and ND6) and three partial (CO1, CO3 and ND5) protein-coding genes. The total length of the sequence of each of the 13 specimens was 4830 bp. The sample consisted of C. osculatum L3 larvae collected from Reinhardtius hippoglossoides and Gadus ogac from the Barents Sea and Davis Strait. The K2P distance values between the species ranged within 0.06–0.12, the intraspecific variability (0.01–0.03) proving 3–6 times lower. The lowest interspecific divergence was observed between C. osculatum A and C. osculatum B, whereas the highest intraspecific diversity was typical of C. osculatum C. Among the C. osculatum species studied, the highest nucleotide diversity was recorded in the CYTB, CO3 and ND5 genes. These genes may be useful in species identification of the very closely related Contracaecum sibling species. Article in Journal/Newspaper Arctic Barents Sea Davis Strait Gadus ogac Cambridge University Press Arctic Barents Sea Journal of Helminthology 86 2 252 258 |
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Open Polar |
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Cambridge University Press |
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crcambridgeupr |
language |
English |
description |
Abstract This study, aimed at testing the hypothesis that some mitochondrial genes can serve as species-specific markers, involved a comparison of the sequence variance of selected mitochondrial DNA genes of the Arctic Contracaecum osculatum species ( C. osculatum A, C. osculatum B and C. osculatum C). We compared differences between five complete (ND2, CYTB, ND3, ND4L and ND6) and three partial (CO1, CO3 and ND5) protein-coding genes. The total length of the sequence of each of the 13 specimens was 4830 bp. The sample consisted of C. osculatum L3 larvae collected from Reinhardtius hippoglossoides and Gadus ogac from the Barents Sea and Davis Strait. The K2P distance values between the species ranged within 0.06–0.12, the intraspecific variability (0.01–0.03) proving 3–6 times lower. The lowest interspecific divergence was observed between C. osculatum A and C. osculatum B, whereas the highest intraspecific diversity was typical of C. osculatum C. Among the C. osculatum species studied, the highest nucleotide diversity was recorded in the CYTB, CO3 and ND5 genes. These genes may be useful in species identification of the very closely related Contracaecum sibling species. |
format |
Article in Journal/Newspaper |
author |
Dzido, J. Kijewska, A. Rokicki, J. |
spellingShingle |
Dzido, J. Kijewska, A. Rokicki, J. Selected mitochondrial genes as species markers of the Arctic Contracaecum osculatumcomplex |
author_facet |
Dzido, J. Kijewska, A. Rokicki, J. |
author_sort |
Dzido, J. |
title |
Selected mitochondrial genes as species markers of the Arctic Contracaecum osculatumcomplex |
title_short |
Selected mitochondrial genes as species markers of the Arctic Contracaecum osculatumcomplex |
title_full |
Selected mitochondrial genes as species markers of the Arctic Contracaecum osculatumcomplex |
title_fullStr |
Selected mitochondrial genes as species markers of the Arctic Contracaecum osculatumcomplex |
title_full_unstemmed |
Selected mitochondrial genes as species markers of the Arctic Contracaecum osculatumcomplex |
title_sort |
selected mitochondrial genes as species markers of the arctic contracaecum osculatumcomplex |
publisher |
Cambridge University Press (CUP) |
publishDate |
2011 |
url |
http://dx.doi.org/10.1017/s0022149x11000332 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0022149X11000332 |
geographic |
Arctic Barents Sea |
geographic_facet |
Arctic Barents Sea |
genre |
Arctic Barents Sea Davis Strait Gadus ogac |
genre_facet |
Arctic Barents Sea Davis Strait Gadus ogac |
op_source |
Journal of Helminthology volume 86, issue 2, page 252-258 ISSN 0022-149X 1475-2697 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0022149x11000332 |
container_title |
Journal of Helminthology |
container_volume |
86 |
container_issue |
2 |
container_start_page |
252 |
op_container_end_page |
258 |
_version_ |
1802640737541554176 |