Genomic identification of Anisakis simplex isolates

• Published in: Journal of Helminthology
• Main Authors: Siles, M., Cuéllar, C., Perteguer, M.J.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Cambridge University Press (CUP) 1997
• Subjects: Animal Science and Zoology; General Medicine; Parasitology; North Atlantic
• Online Access: http://dx.doi.org/10.1017/s0022149x00000833; https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0022149X00000833

Abstract RAPD technique was used to differentiate individuals of Anisakis simplex obtained from Merluccius merluccius, Phycis blennoides, Conger conger and Lepidorhombus boscii , from the North Atlantic Ocean. The amplification patterns of the host DNA controls were markedly different from those obtained for the parasitic material. No variation within the same host was detected. The amplification patterns for larvae obtained from fish of the same genus were somewhat different. The amplification patterns of A. simplex isolates from M. merluccius, P. blennoides, C. conger and L. boscii , were different. These results suggest the possible existence of two populations with a considerable high genetic variability and a different adaptation to different host species.