The long-chain monounsaturated cetoleic acid improves the efficiency of the n-3 fatty acid metabolic pathway in Atlantic salmon and human HepG2 cells
Abstract The present study aimed to determine if the long-chain MUFA cetoleic acid (22 : 1 n -11) can improve the capacity to synthesise the health-promoting n -3 fatty acids EPA and DHA in human and fish models. Human hepatocytes (HepG2) and salmon primary hepatocytes were first enriched with cetol...
Published in: | British Journal of Nutrition |
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2019
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Online Access: | http://dx.doi.org/10.1017/s0007114519001478 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0007114519001478 |
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crcambridgeupr:10.1017/s0007114519001478 2024-09-30T14:32:29+00:00 The long-chain monounsaturated cetoleic acid improves the efficiency of the n-3 fatty acid metabolic pathway in Atlantic salmon and human HepG2 cells Østbye, Tone-Kari Knutsdatter Berge, Gerd Marit Nilsson, Astrid Romarheim, Odd Helge Bou, Marta Ruyter, Bente 2019 http://dx.doi.org/10.1017/s0007114519001478 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0007114519001478 en eng Cambridge University Press (CUP) http://creativecommons.org/licenses/by/4.0/ British Journal of Nutrition volume 122, issue 07, page 755-768 ISSN 0007-1145 1475-2662 journal-article 2019 crcambridgeupr https://doi.org/10.1017/s0007114519001478 2024-09-04T04:04:49Z Abstract The present study aimed to determine if the long-chain MUFA cetoleic acid (22 : 1 n -11) can improve the capacity to synthesise the health-promoting n -3 fatty acids EPA and DHA in human and fish models. Human hepatocytes (HepG2) and salmon primary hepatocytes were first enriched with cetoleic acid, and thereafter their capacities to convert radio-labelled 18 : 3 n -3 ( α -linolenic acid, ALA) to EPA and DHA were measured. Increased endogenous levels of cetoleic acid led to increased production of radio-labelled EPA + DHA in HepG2 by 40 % and EPA in salmon hepatocytes by 12 %. In order to verify if dietary intake of a fish oil rich in cetoleic acid would have the same beneficial effects on the n -3 fatty acid metabolic pathway in vivo as found in vitro , Atlantic salmon were fed four diets supplemented with either sardine oil low in cetoleic acid or herring oil high in cetoleic acid at two inclusion levels (Low or High). The diets were balanced for EPA + DHA content within the Low and within the High groups. The salmon were fed these diets from 110 to 242 g. The level of EPA + DHA in liver and whole-body retention of docosapentaenoic acid and EPA + DHA relative to what was eaten, increased with increased dietary cetoleic acid levels. Thus, it is concluded that cetoleic acid stimulated the synthesis of EPA and DHA from ALA in human HepG2 and of EPA in salmon hepatocytes in vitro and increased whole-body retention of EPA + DHA in salmon by 15 % points after dietary intake of cetoleic acid. Article in Journal/Newspaper Atlantic salmon Cambridge University Press British Journal of Nutrition 122 07 755 768 |
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Cambridge University Press |
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crcambridgeupr |
language |
English |
description |
Abstract The present study aimed to determine if the long-chain MUFA cetoleic acid (22 : 1 n -11) can improve the capacity to synthesise the health-promoting n -3 fatty acids EPA and DHA in human and fish models. Human hepatocytes (HepG2) and salmon primary hepatocytes were first enriched with cetoleic acid, and thereafter their capacities to convert radio-labelled 18 : 3 n -3 ( α -linolenic acid, ALA) to EPA and DHA were measured. Increased endogenous levels of cetoleic acid led to increased production of radio-labelled EPA + DHA in HepG2 by 40 % and EPA in salmon hepatocytes by 12 %. In order to verify if dietary intake of a fish oil rich in cetoleic acid would have the same beneficial effects on the n -3 fatty acid metabolic pathway in vivo as found in vitro , Atlantic salmon were fed four diets supplemented with either sardine oil low in cetoleic acid or herring oil high in cetoleic acid at two inclusion levels (Low or High). The diets were balanced for EPA + DHA content within the Low and within the High groups. The salmon were fed these diets from 110 to 242 g. The level of EPA + DHA in liver and whole-body retention of docosapentaenoic acid and EPA + DHA relative to what was eaten, increased with increased dietary cetoleic acid levels. Thus, it is concluded that cetoleic acid stimulated the synthesis of EPA and DHA from ALA in human HepG2 and of EPA in salmon hepatocytes in vitro and increased whole-body retention of EPA + DHA in salmon by 15 % points after dietary intake of cetoleic acid. |
format |
Article in Journal/Newspaper |
author |
Østbye, Tone-Kari Knutsdatter Berge, Gerd Marit Nilsson, Astrid Romarheim, Odd Helge Bou, Marta Ruyter, Bente |
spellingShingle |
Østbye, Tone-Kari Knutsdatter Berge, Gerd Marit Nilsson, Astrid Romarheim, Odd Helge Bou, Marta Ruyter, Bente The long-chain monounsaturated cetoleic acid improves the efficiency of the n-3 fatty acid metabolic pathway in Atlantic salmon and human HepG2 cells |
author_facet |
Østbye, Tone-Kari Knutsdatter Berge, Gerd Marit Nilsson, Astrid Romarheim, Odd Helge Bou, Marta Ruyter, Bente |
author_sort |
Østbye, Tone-Kari Knutsdatter |
title |
The long-chain monounsaturated cetoleic acid improves the efficiency of the n-3 fatty acid metabolic pathway in Atlantic salmon and human HepG2 cells |
title_short |
The long-chain monounsaturated cetoleic acid improves the efficiency of the n-3 fatty acid metabolic pathway in Atlantic salmon and human HepG2 cells |
title_full |
The long-chain monounsaturated cetoleic acid improves the efficiency of the n-3 fatty acid metabolic pathway in Atlantic salmon and human HepG2 cells |
title_fullStr |
The long-chain monounsaturated cetoleic acid improves the efficiency of the n-3 fatty acid metabolic pathway in Atlantic salmon and human HepG2 cells |
title_full_unstemmed |
The long-chain monounsaturated cetoleic acid improves the efficiency of the n-3 fatty acid metabolic pathway in Atlantic salmon and human HepG2 cells |
title_sort |
long-chain monounsaturated cetoleic acid improves the efficiency of the n-3 fatty acid metabolic pathway in atlantic salmon and human hepg2 cells |
publisher |
Cambridge University Press (CUP) |
publishDate |
2019 |
url |
http://dx.doi.org/10.1017/s0007114519001478 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0007114519001478 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
British Journal of Nutrition volume 122, issue 07, page 755-768 ISSN 0007-1145 1475-2662 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1017/s0007114519001478 |
container_title |
British Journal of Nutrition |
container_volume |
122 |
container_issue |
07 |
container_start_page |
755 |
op_container_end_page |
768 |
_version_ |
1811636632961417216 |