Bioavailability of free lysine and protein-bound lysine from casein and fishmeal in juvenile turbot ( Psetta maxima )
In the present study, a linear regression analysis between lysine intake and lysine retention was conducted to investigate the efficiency of lysine utilisation ( k Lys ) at marginal lysine intake of either protein-bound or free lysine sources in juvenile turbot ( Psetta maxima ). For this purpose, n...
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crcambridgeupr:10.1017/s000711451400381x 2024-03-03T08:49:16+00:00 Bioavailability of free lysine and protein-bound lysine from casein and fishmeal in juvenile turbot ( Psetta maxima ) Kroeckel, Saskia Dietz, Carsten Schulz, Carsten Susenbeth, Andreas 2015 http://dx.doi.org/10.1017/s000711451400381x https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S000711451400381X en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms British Journal of Nutrition volume 113, issue 5, page 718-727 ISSN 0007-1145 1475-2662 Nutrition and Dietetics Medicine (miscellaneous) journal-article 2015 crcambridgeupr https://doi.org/10.1017/s000711451400381x 2024-02-08T08:33:52Z In the present study, a linear regression analysis between lysine intake and lysine retention was conducted to investigate the efficiency of lysine utilisation ( k Lys ) at marginal lysine intake of either protein-bound or free lysine sources in juvenile turbot ( Psetta maxima ). For this purpose, nine isonitrogenous and isoenergetic diets were formulated to contain 2·25–4·12 g lysine/100 g crude protein (CP) to ensure that lysine was the first-limiting amino acid in all diets. The basal diet contained 2·25 g lysine/100 g CP. Graded levels of casein (Cas), fishmeal (FM) and l -lysine HCl (Lys) were added to the experimental diets to achieve stepwise lysine increments. A total of 240 fish (initial weight 50·1 g) were hand-fed all the experimental diets once daily until apparent satiation over a period of 56 d. Feed intake was significantly affected by dietary lysine concentration rather than by dietary lysine source. Specific growth rate increased significantly at higher lysine concentrations ( P < 0·001). CP, crude lipid and crude ash contents in the whole body were affected by the dietary treatments. The linear regression slope between lysine retention and lysine intake ( k Lys ) was similar between all the dietary lysine sources. The k Lys values for the diets supplemented with Cas, Lys or FM were 0·833, 0·857 and 0·684, respectively. The bioavailability of lysine from the respective lysine sources was determined by a slope-ratio approach. The bioavailability of lysine (relative to the reference lysine source Cas) from FM and Lys was 82·1 and 103 %, respectively. Nutrient requirement for maintenance was in the range of 16·7–23·4 mg/kg 0·8 per d, and did not differ between the treatments. There were no significant differences in lysine utilisation efficiency or bioavailability of protein-bound or crystalline lysine from the respective sources observed when lysine was confirmed to be the first-limiting nutrient. Article in Journal/Newspaper Turbot Cambridge University Press British Journal of Nutrition 113 5 718 727 |
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Cambridge University Press |
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English |
topic |
Nutrition and Dietetics Medicine (miscellaneous) |
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Nutrition and Dietetics Medicine (miscellaneous) Kroeckel, Saskia Dietz, Carsten Schulz, Carsten Susenbeth, Andreas Bioavailability of free lysine and protein-bound lysine from casein and fishmeal in juvenile turbot ( Psetta maxima ) |
topic_facet |
Nutrition and Dietetics Medicine (miscellaneous) |
description |
In the present study, a linear regression analysis between lysine intake and lysine retention was conducted to investigate the efficiency of lysine utilisation ( k Lys ) at marginal lysine intake of either protein-bound or free lysine sources in juvenile turbot ( Psetta maxima ). For this purpose, nine isonitrogenous and isoenergetic diets were formulated to contain 2·25–4·12 g lysine/100 g crude protein (CP) to ensure that lysine was the first-limiting amino acid in all diets. The basal diet contained 2·25 g lysine/100 g CP. Graded levels of casein (Cas), fishmeal (FM) and l -lysine HCl (Lys) were added to the experimental diets to achieve stepwise lysine increments. A total of 240 fish (initial weight 50·1 g) were hand-fed all the experimental diets once daily until apparent satiation over a period of 56 d. Feed intake was significantly affected by dietary lysine concentration rather than by dietary lysine source. Specific growth rate increased significantly at higher lysine concentrations ( P < 0·001). CP, crude lipid and crude ash contents in the whole body were affected by the dietary treatments. The linear regression slope between lysine retention and lysine intake ( k Lys ) was similar between all the dietary lysine sources. The k Lys values for the diets supplemented with Cas, Lys or FM were 0·833, 0·857 and 0·684, respectively. The bioavailability of lysine from the respective lysine sources was determined by a slope-ratio approach. The bioavailability of lysine (relative to the reference lysine source Cas) from FM and Lys was 82·1 and 103 %, respectively. Nutrient requirement for maintenance was in the range of 16·7–23·4 mg/kg 0·8 per d, and did not differ between the treatments. There were no significant differences in lysine utilisation efficiency or bioavailability of protein-bound or crystalline lysine from the respective sources observed when lysine was confirmed to be the first-limiting nutrient. |
format |
Article in Journal/Newspaper |
author |
Kroeckel, Saskia Dietz, Carsten Schulz, Carsten Susenbeth, Andreas |
author_facet |
Kroeckel, Saskia Dietz, Carsten Schulz, Carsten Susenbeth, Andreas |
author_sort |
Kroeckel, Saskia |
title |
Bioavailability of free lysine and protein-bound lysine from casein and fishmeal in juvenile turbot ( Psetta maxima ) |
title_short |
Bioavailability of free lysine and protein-bound lysine from casein and fishmeal in juvenile turbot ( Psetta maxima ) |
title_full |
Bioavailability of free lysine and protein-bound lysine from casein and fishmeal in juvenile turbot ( Psetta maxima ) |
title_fullStr |
Bioavailability of free lysine and protein-bound lysine from casein and fishmeal in juvenile turbot ( Psetta maxima ) |
title_full_unstemmed |
Bioavailability of free lysine and protein-bound lysine from casein and fishmeal in juvenile turbot ( Psetta maxima ) |
title_sort |
bioavailability of free lysine and protein-bound lysine from casein and fishmeal in juvenile turbot ( psetta maxima ) |
publisher |
Cambridge University Press (CUP) |
publishDate |
2015 |
url |
http://dx.doi.org/10.1017/s000711451400381x https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S000711451400381X |
genre |
Turbot |
genre_facet |
Turbot |
op_source |
British Journal of Nutrition volume 113, issue 5, page 718-727 ISSN 0007-1145 1475-2662 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s000711451400381x |
container_title |
British Journal of Nutrition |
container_volume |
113 |
container_issue |
5 |
container_start_page |
718 |
op_container_end_page |
727 |
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1792506451739017216 |