Methionine deficiency does not increase polyamine turnover through depletion of hepatic S-adenosylmethionine in juvenile Atlantic salmon
During the last few decades, plant protein ingredients such as soya proteins have replaced fishmeal in the diets of aquacultured species. This may affect the requirement and metabolism of methionine as soya contains less methionine compared with fishmeal. To assess whether methionine limitation affe...
Published in: | British Journal of Nutrition |
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2014
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Online Access: | http://dx.doi.org/10.1017/s0007114514002062 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0007114514002062 |
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crcambridgeupr:10.1017/s0007114514002062 2024-09-15T17:56:17+00:00 Methionine deficiency does not increase polyamine turnover through depletion of hepatic S-adenosylmethionine in juvenile Atlantic salmon Espe, Marit Andersen, Synne Marte Holen, Elisabeth Rønnestad, Ivar Veiseth-Kent, Eva Zerrahn, Jens-Erik Aksnes, Anders 2014 http://dx.doi.org/10.1017/s0007114514002062 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0007114514002062 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms British Journal of Nutrition volume 112, issue 8, page 1274-1285 ISSN 0007-1145 1475-2662 journal-article 2014 crcambridgeupr https://doi.org/10.1017/s0007114514002062 2024-08-07T04:03:58Z During the last few decades, plant protein ingredients such as soya proteins have replaced fishmeal in the diets of aquacultured species. This may affect the requirement and metabolism of methionine as soya contains less methionine compared with fishmeal. To assess whether methionine limitation affects decarboxylated S -adenosylmethionine availability and polyamine status, in the present study, juvenile Atlantic salmon were fed a methionine-deficient plant protein-based diet or the same diet supplemented with dl -methionine for 8 weeks. The test diets were compared with a fishmeal-based control diet to assess their effects on the growth performance of fish. Methionine limitation reduced growth and protein accretion, but when fish were fed the dl -methionine-supplemented diet their growth and protein accretion equalled those of fish fed the fishmeal-based control diet. Methionine limitation reduced free methionine concentrations in the plasma and muscle, while those in the liver were not affected. S -adenosylmethionine (SAM) concentrations were higher in the liver of fish fed the methionine-deficient diet, while S -adenosylhomocysteine concentrations were not affected. Putrescine concentrations were higher and spermine concentrations were lower in the liver of fish fed the methionine-deficient diet, while the gene expression of SAM decarboxylase ( SAMdc ) and the rate-limiting enzyme of polyamine synthesis ornithine decarboxylase ( ODC ) was not affected. Polyamine turnover, as assessed by spermine/spermidine acetyltransferase (SSAT) abundance, activity and gene expression, was not affected by treatment. However, the gene expression of the cytokine TNF-α increased in fish fed the methionine-deficient diet, indicative of stressful conditions in the liver. Even though taurine concentrations in the liver were not affected by treatment, methionine and taurine concentrations in muscle decreased due to methionine deficiency. Concomitantly, liver phospholipid and cholesterol concentrations were reduced, while NEFA ... Article in Journal/Newspaper Atlantic salmon Cambridge University Press British Journal of Nutrition 112 8 1274 1285 |
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Open Polar |
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Cambridge University Press |
op_collection_id |
crcambridgeupr |
language |
English |
description |
During the last few decades, plant protein ingredients such as soya proteins have replaced fishmeal in the diets of aquacultured species. This may affect the requirement and metabolism of methionine as soya contains less methionine compared with fishmeal. To assess whether methionine limitation affects decarboxylated S -adenosylmethionine availability and polyamine status, in the present study, juvenile Atlantic salmon were fed a methionine-deficient plant protein-based diet or the same diet supplemented with dl -methionine for 8 weeks. The test diets were compared with a fishmeal-based control diet to assess their effects on the growth performance of fish. Methionine limitation reduced growth and protein accretion, but when fish were fed the dl -methionine-supplemented diet their growth and protein accretion equalled those of fish fed the fishmeal-based control diet. Methionine limitation reduced free methionine concentrations in the plasma and muscle, while those in the liver were not affected. S -adenosylmethionine (SAM) concentrations were higher in the liver of fish fed the methionine-deficient diet, while S -adenosylhomocysteine concentrations were not affected. Putrescine concentrations were higher and spermine concentrations were lower in the liver of fish fed the methionine-deficient diet, while the gene expression of SAM decarboxylase ( SAMdc ) and the rate-limiting enzyme of polyamine synthesis ornithine decarboxylase ( ODC ) was not affected. Polyamine turnover, as assessed by spermine/spermidine acetyltransferase (SSAT) abundance, activity and gene expression, was not affected by treatment. However, the gene expression of the cytokine TNF-α increased in fish fed the methionine-deficient diet, indicative of stressful conditions in the liver. Even though taurine concentrations in the liver were not affected by treatment, methionine and taurine concentrations in muscle decreased due to methionine deficiency. Concomitantly, liver phospholipid and cholesterol concentrations were reduced, while NEFA ... |
format |
Article in Journal/Newspaper |
author |
Espe, Marit Andersen, Synne Marte Holen, Elisabeth Rønnestad, Ivar Veiseth-Kent, Eva Zerrahn, Jens-Erik Aksnes, Anders |
spellingShingle |
Espe, Marit Andersen, Synne Marte Holen, Elisabeth Rønnestad, Ivar Veiseth-Kent, Eva Zerrahn, Jens-Erik Aksnes, Anders Methionine deficiency does not increase polyamine turnover through depletion of hepatic S-adenosylmethionine in juvenile Atlantic salmon |
author_facet |
Espe, Marit Andersen, Synne Marte Holen, Elisabeth Rønnestad, Ivar Veiseth-Kent, Eva Zerrahn, Jens-Erik Aksnes, Anders |
author_sort |
Espe, Marit |
title |
Methionine deficiency does not increase polyamine turnover through depletion of hepatic S-adenosylmethionine in juvenile Atlantic salmon |
title_short |
Methionine deficiency does not increase polyamine turnover through depletion of hepatic S-adenosylmethionine in juvenile Atlantic salmon |
title_full |
Methionine deficiency does not increase polyamine turnover through depletion of hepatic S-adenosylmethionine in juvenile Atlantic salmon |
title_fullStr |
Methionine deficiency does not increase polyamine turnover through depletion of hepatic S-adenosylmethionine in juvenile Atlantic salmon |
title_full_unstemmed |
Methionine deficiency does not increase polyamine turnover through depletion of hepatic S-adenosylmethionine in juvenile Atlantic salmon |
title_sort |
methionine deficiency does not increase polyamine turnover through depletion of hepatic s-adenosylmethionine in juvenile atlantic salmon |
publisher |
Cambridge University Press (CUP) |
publishDate |
2014 |
url |
http://dx.doi.org/10.1017/s0007114514002062 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0007114514002062 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
British Journal of Nutrition volume 112, issue 8, page 1274-1285 ISSN 0007-1145 1475-2662 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0007114514002062 |
container_title |
British Journal of Nutrition |
container_volume |
112 |
container_issue |
8 |
container_start_page |
1274 |
op_container_end_page |
1285 |
_version_ |
1810432497540923392 |