Transcriptional regulation of cholesterol and bile acid metabolism after dietary soyabean meal treatment in Atlantic salmon ( Salmo salar L.)
Inclusion of plant protein sources such as soyabean meal (SBM) in aquafeeds is associated with decreased lipid digestibility, reduced bile acid levels and hypocholesterolaemia. The mechanism for these metabolic abnormalities is unknown. The present study aimed at gaining further insight into how cho...
Published in: | British Journal of Nutrition |
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Online Access: | http://dx.doi.org/10.1017/s0007114512002024 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0007114512002024 |
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crcambridgeupr:10.1017/s0007114512002024 2024-10-06T13:47:22+00:00 Transcriptional regulation of cholesterol and bile acid metabolism after dietary soyabean meal treatment in Atlantic salmon ( Salmo salar L.) Kortner, Trond M. Gu, Jinni Krogdahl, Åshild Bakke, Anne Marie 2012 http://dx.doi.org/10.1017/s0007114512002024 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0007114512002024 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms British Journal of Nutrition volume 109, issue 4, page 593-604 ISSN 0007-1145 1475-2662 journal-article 2012 crcambridgeupr https://doi.org/10.1017/s0007114512002024 2024-09-11T04:03:43Z Inclusion of plant protein sources such as soyabean meal (SBM) in aquafeeds is associated with decreased lipid digestibility, reduced bile acid levels and hypocholesterolaemia. The mechanism for these metabolic abnormalities is unknown. The present study aimed at gaining further insight into how cholesterol and bile acid metabolism is modulated by SBM feeding by quantifying a number of mRNA species corresponding to key proteins involved in cholesterol and bile acid metabolism using quantitative real-time PCR. A 21 d feeding trial with sequential sampling at ten time points following initiation of 20 % SBM exposure was conducted on Atlantic salmon. A histological evaluation confirmed distal intestinal enteritis after 5 d of dietary exposure to the SBM, whereas diminished glycogen/lipid deposition was the only relevant finding observed in the liver. SBM inclusion resulted in reduced body pools of cholesterol and bile acids. Hepatic gene expression profiles revealed up-regulation of genes encoding rate-limiting enzymes in cholesterol (3-hydroxy-3-methyl-glutaryl-CoA reductase; HMGCR) and bile acid (cytochrome P4507A1 (CYP7A1)) biosynthesis, as well as up-regulation of their associated transcription factors (sterol regulatory element binding proteins 1 and 2, liver X receptor, farnesoid X receptor and PPAR isoforms). Hepatic gene expressions of cholesterol (ATP binding cassette G5 (ABCG5)) and bile acid (ATP binding cassette B11 (ABCB11)) transporters were, by and large, not influenced by the SBM, but distal intestinal expression patterns of ABCG5 and apical Na-dependent bile acid transporter indicated impaired cholesterol and bile acid reabsorption. In conclusion, hepatic gene expression profiles indicated that the capacity for cholesterol and bile acid synthesis was up-regulated, whereas the indicated impaired cholesterol and bile acid reabsorption probably occurred as a direct result of distal intestinal inflammation. Article in Journal/Newspaper Atlantic salmon Salmo salar Cambridge University Press British Journal of Nutrition 109 4 593 604 |
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Open Polar |
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Cambridge University Press |
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crcambridgeupr |
language |
English |
description |
Inclusion of plant protein sources such as soyabean meal (SBM) in aquafeeds is associated with decreased lipid digestibility, reduced bile acid levels and hypocholesterolaemia. The mechanism for these metabolic abnormalities is unknown. The present study aimed at gaining further insight into how cholesterol and bile acid metabolism is modulated by SBM feeding by quantifying a number of mRNA species corresponding to key proteins involved in cholesterol and bile acid metabolism using quantitative real-time PCR. A 21 d feeding trial with sequential sampling at ten time points following initiation of 20 % SBM exposure was conducted on Atlantic salmon. A histological evaluation confirmed distal intestinal enteritis after 5 d of dietary exposure to the SBM, whereas diminished glycogen/lipid deposition was the only relevant finding observed in the liver. SBM inclusion resulted in reduced body pools of cholesterol and bile acids. Hepatic gene expression profiles revealed up-regulation of genes encoding rate-limiting enzymes in cholesterol (3-hydroxy-3-methyl-glutaryl-CoA reductase; HMGCR) and bile acid (cytochrome P4507A1 (CYP7A1)) biosynthesis, as well as up-regulation of their associated transcription factors (sterol regulatory element binding proteins 1 and 2, liver X receptor, farnesoid X receptor and PPAR isoforms). Hepatic gene expressions of cholesterol (ATP binding cassette G5 (ABCG5)) and bile acid (ATP binding cassette B11 (ABCB11)) transporters were, by and large, not influenced by the SBM, but distal intestinal expression patterns of ABCG5 and apical Na-dependent bile acid transporter indicated impaired cholesterol and bile acid reabsorption. In conclusion, hepatic gene expression profiles indicated that the capacity for cholesterol and bile acid synthesis was up-regulated, whereas the indicated impaired cholesterol and bile acid reabsorption probably occurred as a direct result of distal intestinal inflammation. |
format |
Article in Journal/Newspaper |
author |
Kortner, Trond M. Gu, Jinni Krogdahl, Åshild Bakke, Anne Marie |
spellingShingle |
Kortner, Trond M. Gu, Jinni Krogdahl, Åshild Bakke, Anne Marie Transcriptional regulation of cholesterol and bile acid metabolism after dietary soyabean meal treatment in Atlantic salmon ( Salmo salar L.) |
author_facet |
Kortner, Trond M. Gu, Jinni Krogdahl, Åshild Bakke, Anne Marie |
author_sort |
Kortner, Trond M. |
title |
Transcriptional regulation of cholesterol and bile acid metabolism after dietary soyabean meal treatment in Atlantic salmon ( Salmo salar L.) |
title_short |
Transcriptional regulation of cholesterol and bile acid metabolism after dietary soyabean meal treatment in Atlantic salmon ( Salmo salar L.) |
title_full |
Transcriptional regulation of cholesterol and bile acid metabolism after dietary soyabean meal treatment in Atlantic salmon ( Salmo salar L.) |
title_fullStr |
Transcriptional regulation of cholesterol and bile acid metabolism after dietary soyabean meal treatment in Atlantic salmon ( Salmo salar L.) |
title_full_unstemmed |
Transcriptional regulation of cholesterol and bile acid metabolism after dietary soyabean meal treatment in Atlantic salmon ( Salmo salar L.) |
title_sort |
transcriptional regulation of cholesterol and bile acid metabolism after dietary soyabean meal treatment in atlantic salmon ( salmo salar l.) |
publisher |
Cambridge University Press (CUP) |
publishDate |
2012 |
url |
http://dx.doi.org/10.1017/s0007114512002024 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0007114512002024 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
British Journal of Nutrition volume 109, issue 4, page 593-604 ISSN 0007-1145 1475-2662 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0007114512002024 |
container_title |
British Journal of Nutrition |
container_volume |
109 |
container_issue |
4 |
container_start_page |
593 |
op_container_end_page |
604 |
_version_ |
1812175591846182912 |