The effects of prebiotics on the digestive enzymes and gut histomorphology of red drum ( Sciaenops ocellatus) and hybrid striped bass ( Morone chrysops× M. saxatilis)
The effects of four prebiotics (fructo-oligosaccharide, Bio-MOS ® , transgalacto-oligosaccharide and GroBiotic ® -A) on digestive enzymes and intestinal morphology were studied in juvenile hybrid striped bass ( Morone chrysops × M. saxatilis ) and red drum ( Sciaenops ocellatus ) using two separate...
Published in: | British Journal of Nutrition |
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Language: | English |
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Cambridge University Press (CUP)
2012
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Online Access: | http://dx.doi.org/10.1017/s0007114512001754 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0007114512001754 |
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crcambridgeupr:10.1017/s0007114512001754 2024-09-15T18:32:12+00:00 The effects of prebiotics on the digestive enzymes and gut histomorphology of red drum ( Sciaenops ocellatus) and hybrid striped bass ( Morone chrysops× M. saxatilis) Anguiano, Maritza Pohlenz, Camilo Buentello, Alejandro Gatlin, Delbert M. 2012 http://dx.doi.org/10.1017/s0007114512001754 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0007114512001754 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms British Journal of Nutrition volume 109, issue 4, page 623-629 ISSN 0007-1145 1475-2662 journal-article 2012 crcambridgeupr https://doi.org/10.1017/s0007114512001754 2024-08-21T04:04:03Z The effects of four prebiotics (fructo-oligosaccharide, Bio-MOS ® , transgalacto-oligosaccharide and GroBiotic ® -A) on digestive enzymes and intestinal morphology were studied in juvenile hybrid striped bass ( Morone chrysops × M. saxatilis ) and red drum ( Sciaenops ocellatus ) using two separate 8-week feeding trials. Red drum were fed experimental diets with the four prebiotics each individually supplemented at 1 % and hybrid striped bass were fed diets supplemented with GroBiotic ® -A at 1 and 2 %. Both trials were conducted with each diet fed to apparent satiation twice per d to three replicate groups of fifteen juvenile fish. For histomorphological analysis, gastrointestinal tract (GIT) samples from three randomly selected fish per tank were taken at 4 and 8 weeks for hybrid striped bass and at 8 weeks for red drum. For both trials, GIT samples from two randomly selected fish per tank were taken at 4 and 8 weeks and analysed for pepsin, trypsin, chymotrypsin, aminopeptidase, α-amylase, lipase, and both acid and alkaline phosphatase activities. The results of the histological evaluation indicated that the inclusion of prebiotics was adequate to elicit structural changes in the GIT of both species. On the other hand, no significant changes in the enzyme activities were detected at week 8 in both species. However, there was a transient effect of Bio-MOS ® supplementation on the activities of aminopeptidase, α-amylase and alkaline phosphatase at week 4 in red drum only. Thus, previously observed improvements in nutrient digestibility by these fish in response to prebiotic supplementation appear to be mostly related to changes in GIT structure as opposed to the enhancement of digestive enzyme activity. Article in Journal/Newspaper Red drum Sciaenops ocellatus Cambridge University Press British Journal of Nutrition 109 4 623 629 |
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Open Polar |
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Cambridge University Press |
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crcambridgeupr |
language |
English |
description |
The effects of four prebiotics (fructo-oligosaccharide, Bio-MOS ® , transgalacto-oligosaccharide and GroBiotic ® -A) on digestive enzymes and intestinal morphology were studied in juvenile hybrid striped bass ( Morone chrysops × M. saxatilis ) and red drum ( Sciaenops ocellatus ) using two separate 8-week feeding trials. Red drum were fed experimental diets with the four prebiotics each individually supplemented at 1 % and hybrid striped bass were fed diets supplemented with GroBiotic ® -A at 1 and 2 %. Both trials were conducted with each diet fed to apparent satiation twice per d to three replicate groups of fifteen juvenile fish. For histomorphological analysis, gastrointestinal tract (GIT) samples from three randomly selected fish per tank were taken at 4 and 8 weeks for hybrid striped bass and at 8 weeks for red drum. For both trials, GIT samples from two randomly selected fish per tank were taken at 4 and 8 weeks and analysed for pepsin, trypsin, chymotrypsin, aminopeptidase, α-amylase, lipase, and both acid and alkaline phosphatase activities. The results of the histological evaluation indicated that the inclusion of prebiotics was adequate to elicit structural changes in the GIT of both species. On the other hand, no significant changes in the enzyme activities were detected at week 8 in both species. However, there was a transient effect of Bio-MOS ® supplementation on the activities of aminopeptidase, α-amylase and alkaline phosphatase at week 4 in red drum only. Thus, previously observed improvements in nutrient digestibility by these fish in response to prebiotic supplementation appear to be mostly related to changes in GIT structure as opposed to the enhancement of digestive enzyme activity. |
format |
Article in Journal/Newspaper |
author |
Anguiano, Maritza Pohlenz, Camilo Buentello, Alejandro Gatlin, Delbert M. |
spellingShingle |
Anguiano, Maritza Pohlenz, Camilo Buentello, Alejandro Gatlin, Delbert M. The effects of prebiotics on the digestive enzymes and gut histomorphology of red drum ( Sciaenops ocellatus) and hybrid striped bass ( Morone chrysops× M. saxatilis) |
author_facet |
Anguiano, Maritza Pohlenz, Camilo Buentello, Alejandro Gatlin, Delbert M. |
author_sort |
Anguiano, Maritza |
title |
The effects of prebiotics on the digestive enzymes and gut histomorphology of red drum ( Sciaenops ocellatus) and hybrid striped bass ( Morone chrysops× M. saxatilis) |
title_short |
The effects of prebiotics on the digestive enzymes and gut histomorphology of red drum ( Sciaenops ocellatus) and hybrid striped bass ( Morone chrysops× M. saxatilis) |
title_full |
The effects of prebiotics on the digestive enzymes and gut histomorphology of red drum ( Sciaenops ocellatus) and hybrid striped bass ( Morone chrysops× M. saxatilis) |
title_fullStr |
The effects of prebiotics on the digestive enzymes and gut histomorphology of red drum ( Sciaenops ocellatus) and hybrid striped bass ( Morone chrysops× M. saxatilis) |
title_full_unstemmed |
The effects of prebiotics on the digestive enzymes and gut histomorphology of red drum ( Sciaenops ocellatus) and hybrid striped bass ( Morone chrysops× M. saxatilis) |
title_sort |
effects of prebiotics on the digestive enzymes and gut histomorphology of red drum ( sciaenops ocellatus) and hybrid striped bass ( morone chrysops× m. saxatilis) |
publisher |
Cambridge University Press (CUP) |
publishDate |
2012 |
url |
http://dx.doi.org/10.1017/s0007114512001754 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0007114512001754 |
genre |
Red drum Sciaenops ocellatus |
genre_facet |
Red drum Sciaenops ocellatus |
op_source |
British Journal of Nutrition volume 109, issue 4, page 623-629 ISSN 0007-1145 1475-2662 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0007114512001754 |
container_title |
British Journal of Nutrition |
container_volume |
109 |
container_issue |
4 |
container_start_page |
623 |
op_container_end_page |
629 |
_version_ |
1810473949679583232 |