Diet × genotype interactions in hepatic cholesterol and lipoprotein metabolism in Atlantic salmon ( Salmo salar) in response to replacement of dietary fish oil with vegetable oil
The present study investigates the effects of genotype on responses to alternative feeds in Atlantic salmon. Microarray analysis of the liver transcriptome of two family groups, lean or fat, fed a diet containing either a fish oil (FO) or a vegetable oil (VO) blend indicated that pathways of cholest...
Published in: | British Journal of Nutrition |
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Language: | English |
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Cambridge University Press (CUP)
2011
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Online Access: | http://dx.doi.org/10.1017/s0007114511001954 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0007114511001954 |
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crcambridgeupr:10.1017/s0007114511001954 2024-09-15T17:56:17+00:00 Diet × genotype interactions in hepatic cholesterol and lipoprotein metabolism in Atlantic salmon ( Salmo salar) in response to replacement of dietary fish oil with vegetable oil Morais, Sofia Pratoomyot, Jarunan Torstensen, Bente E. Taggart, John B. Guy, Derrick R. Gordon Bell, J. Tocher, Douglas R. 2011 http://dx.doi.org/10.1017/s0007114511001954 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0007114511001954 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms British Journal of Nutrition volume 106, issue 10, page 1457-1469 ISSN 0007-1145 1475-2662 journal-article 2011 crcambridgeupr https://doi.org/10.1017/s0007114511001954 2024-08-21T04:03:12Z The present study investigates the effects of genotype on responses to alternative feeds in Atlantic salmon. Microarray analysis of the liver transcriptome of two family groups, lean or fat, fed a diet containing either a fish oil (FO) or a vegetable oil (VO) blend indicated that pathways of cholesterol and lipoprotein metabolism might be differentially affected by the diet depending on the genetic background of the fish, and this was further investigated by real-time quantitative PCR, plasma and lipoprotein biochemical analysis. Results indicate a reduction in VLDL and LDL levels, with no changes in HDL, when FO is replaced by VO in the lean family group, whereas in fat fish fed FO, levels of apoB-containing lipoproteins were low and comparable with those fed VO in both family groups. Significantly lower levels of plasma TAG and LDL-TAG were measured in the fat group that was independent of diet, whereas plasma cholesterol was significantly higher in fish fed the FO diet in both groups. Hepatic expression of genes involved in cholesterol homeostasis, β-oxidation and lipoprotein metabolism showed relatively subtle changes. A significantly lower expression of genes considered anti-atherogenic in mammals (ATP-binding cassette transporter A1, apoAI, scavenger receptor class B type 1, lipoprotein lipase (LPL)b (TC67836) and LPLc (TC84899)) was found in lean fish, compared with fat fish, when fed VO. Furthermore, the lean family group appeared to show a greater response to diet composition in the cholesterol biosynthesis pathway, mediated by sterol-responsive element-binding protein 2. Finally, the presence of three different transcripts for LPL, with differential patterns of nutritional regulation, was demonstrated. Article in Journal/Newspaper Atlantic salmon Salmo salar Cambridge University Press British Journal of Nutrition 106 10 1457 1469 |
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Cambridge University Press |
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crcambridgeupr |
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English |
description |
The present study investigates the effects of genotype on responses to alternative feeds in Atlantic salmon. Microarray analysis of the liver transcriptome of two family groups, lean or fat, fed a diet containing either a fish oil (FO) or a vegetable oil (VO) blend indicated that pathways of cholesterol and lipoprotein metabolism might be differentially affected by the diet depending on the genetic background of the fish, and this was further investigated by real-time quantitative PCR, plasma and lipoprotein biochemical analysis. Results indicate a reduction in VLDL and LDL levels, with no changes in HDL, when FO is replaced by VO in the lean family group, whereas in fat fish fed FO, levels of apoB-containing lipoproteins were low and comparable with those fed VO in both family groups. Significantly lower levels of plasma TAG and LDL-TAG were measured in the fat group that was independent of diet, whereas plasma cholesterol was significantly higher in fish fed the FO diet in both groups. Hepatic expression of genes involved in cholesterol homeostasis, β-oxidation and lipoprotein metabolism showed relatively subtle changes. A significantly lower expression of genes considered anti-atherogenic in mammals (ATP-binding cassette transporter A1, apoAI, scavenger receptor class B type 1, lipoprotein lipase (LPL)b (TC67836) and LPLc (TC84899)) was found in lean fish, compared with fat fish, when fed VO. Furthermore, the lean family group appeared to show a greater response to diet composition in the cholesterol biosynthesis pathway, mediated by sterol-responsive element-binding protein 2. Finally, the presence of three different transcripts for LPL, with differential patterns of nutritional regulation, was demonstrated. |
format |
Article in Journal/Newspaper |
author |
Morais, Sofia Pratoomyot, Jarunan Torstensen, Bente E. Taggart, John B. Guy, Derrick R. Gordon Bell, J. Tocher, Douglas R. |
spellingShingle |
Morais, Sofia Pratoomyot, Jarunan Torstensen, Bente E. Taggart, John B. Guy, Derrick R. Gordon Bell, J. Tocher, Douglas R. Diet × genotype interactions in hepatic cholesterol and lipoprotein metabolism in Atlantic salmon ( Salmo salar) in response to replacement of dietary fish oil with vegetable oil |
author_facet |
Morais, Sofia Pratoomyot, Jarunan Torstensen, Bente E. Taggart, John B. Guy, Derrick R. Gordon Bell, J. Tocher, Douglas R. |
author_sort |
Morais, Sofia |
title |
Diet × genotype interactions in hepatic cholesterol and lipoprotein metabolism in Atlantic salmon ( Salmo salar) in response to replacement of dietary fish oil with vegetable oil |
title_short |
Diet × genotype interactions in hepatic cholesterol and lipoprotein metabolism in Atlantic salmon ( Salmo salar) in response to replacement of dietary fish oil with vegetable oil |
title_full |
Diet × genotype interactions in hepatic cholesterol and lipoprotein metabolism in Atlantic salmon ( Salmo salar) in response to replacement of dietary fish oil with vegetable oil |
title_fullStr |
Diet × genotype interactions in hepatic cholesterol and lipoprotein metabolism in Atlantic salmon ( Salmo salar) in response to replacement of dietary fish oil with vegetable oil |
title_full_unstemmed |
Diet × genotype interactions in hepatic cholesterol and lipoprotein metabolism in Atlantic salmon ( Salmo salar) in response to replacement of dietary fish oil with vegetable oil |
title_sort |
diet × genotype interactions in hepatic cholesterol and lipoprotein metabolism in atlantic salmon ( salmo salar) in response to replacement of dietary fish oil with vegetable oil |
publisher |
Cambridge University Press (CUP) |
publishDate |
2011 |
url |
http://dx.doi.org/10.1017/s0007114511001954 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0007114511001954 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
British Journal of Nutrition volume 106, issue 10, page 1457-1469 ISSN 0007-1145 1475-2662 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0007114511001954 |
container_title |
British Journal of Nutrition |
container_volume |
106 |
container_issue |
10 |
container_start_page |
1457 |
op_container_end_page |
1469 |
_version_ |
1810432492234080256 |