Hypocholesterolaemic effect of water-insoluble fish protein from Alaska pollock in ovariectomised rats is not abolished by methionine addition
The present study investigated whether the hypocholesterolaemic effect of water-insoluble fish protein (IFP) from Alaska pollock in ovariectomised (OVX) rats was affected by methionine (Met) addition. OVX rats (6 months old) were fed a cholesterol-free diet containing casein, IFP or IFP+Met as a pro...
Published in: | British Journal of Nutrition |
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Language: | English |
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Cambridge University Press (CUP)
2011
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Online Access: | http://dx.doi.org/10.1017/s0007114510005775 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0007114510005775 |
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crcambridgeupr:10.1017/s0007114510005775 2024-06-23T07:45:03+00:00 Hypocholesterolaemic effect of water-insoluble fish protein from Alaska pollock in ovariectomised rats is not abolished by methionine addition Kato, Masaki Ogawa, Hiroshi Kishida, Taro Ebihara, Kiyoshi 2011 http://dx.doi.org/10.1017/s0007114510005775 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0007114510005775 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms British Journal of Nutrition volume 106, issue 1, page 57-62 ISSN 0007-1145 1475-2662 journal-article 2011 crcambridgeupr https://doi.org/10.1017/s0007114510005775 2024-06-12T04:04:37Z The present study investigated whether the hypocholesterolaemic effect of water-insoluble fish protein (IFP) from Alaska pollock in ovariectomised (OVX) rats was affected by methionine (Met) addition. OVX rats (6 months old) were fed a cholesterol-free diet containing casein, IFP or IFP+Met as a protein source for 28 d. The ratio of Met:glycine was lower in the IFP and IFP+Met diets compared with the casein diet. Body-weight gain, food intake and liver lipids were not affected by the diet. Plasma total cholesterol concentration was lower in OVX rats fed the IFP diet compared with those fed the casein diet. The hypocholesterolaemic effect of the IFP diet was not abolished by Met addition. Amount of bile acids in the small-intestinal content and faecal excretion of bile acids were higher in OVX rats fed the IFP and IFP+Met diets compared with those fed the casein diet. Ileal bile acid transporter (IBAT) mRNA level and faecal excretion of bile acids were significantly lower and higher, respectively, in OVX rats fed the IFP diet compared with those fed the casein diet, but not in those fed the IFP+Met diet. Thus, the hypocholesterolaemic effect of the IFP diet seems to be mediated by increased faecal excretion of bile acids coupled with decreased reabsorption of bile acids from the ileum through a decrease in IBAT and the change in cholesterol metabolism linked to the amino acid profile. Article in Journal/Newspaper alaska pollock Alaska Cambridge University Press British Journal of Nutrition 106 1 57 62 |
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Open Polar |
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Cambridge University Press |
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crcambridgeupr |
language |
English |
description |
The present study investigated whether the hypocholesterolaemic effect of water-insoluble fish protein (IFP) from Alaska pollock in ovariectomised (OVX) rats was affected by methionine (Met) addition. OVX rats (6 months old) were fed a cholesterol-free diet containing casein, IFP or IFP+Met as a protein source for 28 d. The ratio of Met:glycine was lower in the IFP and IFP+Met diets compared with the casein diet. Body-weight gain, food intake and liver lipids were not affected by the diet. Plasma total cholesterol concentration was lower in OVX rats fed the IFP diet compared with those fed the casein diet. The hypocholesterolaemic effect of the IFP diet was not abolished by Met addition. Amount of bile acids in the small-intestinal content and faecal excretion of bile acids were higher in OVX rats fed the IFP and IFP+Met diets compared with those fed the casein diet. Ileal bile acid transporter (IBAT) mRNA level and faecal excretion of bile acids were significantly lower and higher, respectively, in OVX rats fed the IFP diet compared with those fed the casein diet, but not in those fed the IFP+Met diet. Thus, the hypocholesterolaemic effect of the IFP diet seems to be mediated by increased faecal excretion of bile acids coupled with decreased reabsorption of bile acids from the ileum through a decrease in IBAT and the change in cholesterol metabolism linked to the amino acid profile. |
format |
Article in Journal/Newspaper |
author |
Kato, Masaki Ogawa, Hiroshi Kishida, Taro Ebihara, Kiyoshi |
spellingShingle |
Kato, Masaki Ogawa, Hiroshi Kishida, Taro Ebihara, Kiyoshi Hypocholesterolaemic effect of water-insoluble fish protein from Alaska pollock in ovariectomised rats is not abolished by methionine addition |
author_facet |
Kato, Masaki Ogawa, Hiroshi Kishida, Taro Ebihara, Kiyoshi |
author_sort |
Kato, Masaki |
title |
Hypocholesterolaemic effect of water-insoluble fish protein from Alaska pollock in ovariectomised rats is not abolished by methionine addition |
title_short |
Hypocholesterolaemic effect of water-insoluble fish protein from Alaska pollock in ovariectomised rats is not abolished by methionine addition |
title_full |
Hypocholesterolaemic effect of water-insoluble fish protein from Alaska pollock in ovariectomised rats is not abolished by methionine addition |
title_fullStr |
Hypocholesterolaemic effect of water-insoluble fish protein from Alaska pollock in ovariectomised rats is not abolished by methionine addition |
title_full_unstemmed |
Hypocholesterolaemic effect of water-insoluble fish protein from Alaska pollock in ovariectomised rats is not abolished by methionine addition |
title_sort |
hypocholesterolaemic effect of water-insoluble fish protein from alaska pollock in ovariectomised rats is not abolished by methionine addition |
publisher |
Cambridge University Press (CUP) |
publishDate |
2011 |
url |
http://dx.doi.org/10.1017/s0007114510005775 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0007114510005775 |
genre |
alaska pollock Alaska |
genre_facet |
alaska pollock Alaska |
op_source |
British Journal of Nutrition volume 106, issue 1, page 57-62 ISSN 0007-1145 1475-2662 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0007114510005775 |
container_title |
British Journal of Nutrition |
container_volume |
106 |
container_issue |
1 |
container_start_page |
57 |
op_container_end_page |
62 |
_version_ |
1802649662307434496 |