Characterisation and expression of secretory phospholipase A 2 group IB during ontogeny of Atlantic cod ( Gadus morhua)

The pancreatic enzyme secretory phospholipase A 2 group IB (sPLA 2 IB) hydrolyses phospholipids at the sn-2 position, resulting in a NEFA and a lyso-phospholipid, which are then absorbed by the enterocytes. The sPLA 2 IB is a member of a family of nineteen enzymes sharing the same catalytic ability,...

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Published in:British Journal of Nutrition
Main Authors: Sæle, Øystein, Nordgreen, Andreas, Olsvik, Pål A., Hamre, Kristin
Format: Article in Journal/Newspaper
Language:English
Published: Cambridge University Press (CUP) 2010
Subjects:
Online Access:http://dx.doi.org/10.1017/s0007114510003466
https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0007114510003466
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spelling crcambridgeupr:10.1017/s0007114510003466 2024-09-15T17:55:27+00:00 Characterisation and expression of secretory phospholipase A 2 group IB during ontogeny of Atlantic cod ( Gadus morhua) Sæle, Øystein Nordgreen, Andreas Olsvik, Pål A. Hamre, Kristin 2010 http://dx.doi.org/10.1017/s0007114510003466 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0007114510003466 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms British Journal of Nutrition volume 105, issue 2, page 228-237 ISSN 0007-1145 1475-2662 journal-article 2010 crcambridgeupr https://doi.org/10.1017/s0007114510003466 2024-07-03T04:03:41Z The pancreatic enzyme secretory phospholipase A 2 group IB (sPLA 2 IB) hydrolyses phospholipids at the sn-2 position, resulting in a NEFA and a lyso-phospholipid, which are then absorbed by the enterocytes. The sPLA 2 IB is a member of a family of nineteen enzymes sharing the same catalytic ability, of which nine are cytosolic and ten are secretory. Presently, there are no pharmacological tools to separate between the different secretory enzymes when measuring the enzymatic activity. Thus, it is important to support activity data with more precise techniques when isolation of intestinal content is not possible for analysis, as in the case of small teleost larvae, where the whole animal is sometimes analysed. In the present study, we characterise the sPLA 2 IB gene in Atlantic cod ( Gadus morhua ) and describe its ontogeny at the genetic and protein level and compare this to the total sPLA 2 activity level. A positive correlation was found between the expression of sPLA 2 IB mRNA and protein. Both remained stable and low during the larval stage followed by an increase from day 62 posthatch, coinciding with the development of the pyloric ceaca. Meanwhile, total sPLA 2 enzyme activity in cod was stable and relatively high during the early stages when larvae were fed live prey, followed by a decrease in activity when the fish were weaned to a formulated diet. Thus, the expression of sPLA 2 IB mRNA and protein did not correlate with total sPLA 2 activity. Article in Journal/Newspaper atlantic cod Gadus morhua Cambridge University Press British Journal of Nutrition 105 2 228 237
institution Open Polar
collection Cambridge University Press
op_collection_id crcambridgeupr
language English
description The pancreatic enzyme secretory phospholipase A 2 group IB (sPLA 2 IB) hydrolyses phospholipids at the sn-2 position, resulting in a NEFA and a lyso-phospholipid, which are then absorbed by the enterocytes. The sPLA 2 IB is a member of a family of nineteen enzymes sharing the same catalytic ability, of which nine are cytosolic and ten are secretory. Presently, there are no pharmacological tools to separate between the different secretory enzymes when measuring the enzymatic activity. Thus, it is important to support activity data with more precise techniques when isolation of intestinal content is not possible for analysis, as in the case of small teleost larvae, where the whole animal is sometimes analysed. In the present study, we characterise the sPLA 2 IB gene in Atlantic cod ( Gadus morhua ) and describe its ontogeny at the genetic and protein level and compare this to the total sPLA 2 activity level. A positive correlation was found between the expression of sPLA 2 IB mRNA and protein. Both remained stable and low during the larval stage followed by an increase from day 62 posthatch, coinciding with the development of the pyloric ceaca. Meanwhile, total sPLA 2 enzyme activity in cod was stable and relatively high during the early stages when larvae were fed live prey, followed by a decrease in activity when the fish were weaned to a formulated diet. Thus, the expression of sPLA 2 IB mRNA and protein did not correlate with total sPLA 2 activity.
format Article in Journal/Newspaper
author Sæle, Øystein
Nordgreen, Andreas
Olsvik, Pål A.
Hamre, Kristin
spellingShingle Sæle, Øystein
Nordgreen, Andreas
Olsvik, Pål A.
Hamre, Kristin
Characterisation and expression of secretory phospholipase A 2 group IB during ontogeny of Atlantic cod ( Gadus morhua)
author_facet Sæle, Øystein
Nordgreen, Andreas
Olsvik, Pål A.
Hamre, Kristin
author_sort Sæle, Øystein
title Characterisation and expression of secretory phospholipase A 2 group IB during ontogeny of Atlantic cod ( Gadus morhua)
title_short Characterisation and expression of secretory phospholipase A 2 group IB during ontogeny of Atlantic cod ( Gadus morhua)
title_full Characterisation and expression of secretory phospholipase A 2 group IB during ontogeny of Atlantic cod ( Gadus morhua)
title_fullStr Characterisation and expression of secretory phospholipase A 2 group IB during ontogeny of Atlantic cod ( Gadus morhua)
title_full_unstemmed Characterisation and expression of secretory phospholipase A 2 group IB during ontogeny of Atlantic cod ( Gadus morhua)
title_sort characterisation and expression of secretory phospholipase a 2 group ib during ontogeny of atlantic cod ( gadus morhua)
publisher Cambridge University Press (CUP)
publishDate 2010
url http://dx.doi.org/10.1017/s0007114510003466
https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0007114510003466
genre atlantic cod
Gadus morhua
genre_facet atlantic cod
Gadus morhua
op_source British Journal of Nutrition
volume 105, issue 2, page 228-237
ISSN 0007-1145 1475-2662
op_rights https://www.cambridge.org/core/terms
op_doi https://doi.org/10.1017/s0007114510003466
container_title British Journal of Nutrition
container_volume 105
container_issue 2
container_start_page 228
op_container_end_page 237
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