Characterisation and expression of secretory phospholipase A 2 group IB during ontogeny of Atlantic cod ( Gadus morhua)
The pancreatic enzyme secretory phospholipase A 2 group IB (sPLA 2 IB) hydrolyses phospholipids at the sn-2 position, resulting in a NEFA and a lyso-phospholipid, which are then absorbed by the enterocytes. The sPLA 2 IB is a member of a family of nineteen enzymes sharing the same catalytic ability,...
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2010
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Online Access: | http://dx.doi.org/10.1017/s0007114510003466 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0007114510003466 |
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crcambridgeupr:10.1017/s0007114510003466 2024-09-15T17:55:27+00:00 Characterisation and expression of secretory phospholipase A 2 group IB during ontogeny of Atlantic cod ( Gadus morhua) Sæle, Øystein Nordgreen, Andreas Olsvik, Pål A. Hamre, Kristin 2010 http://dx.doi.org/10.1017/s0007114510003466 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0007114510003466 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms British Journal of Nutrition volume 105, issue 2, page 228-237 ISSN 0007-1145 1475-2662 journal-article 2010 crcambridgeupr https://doi.org/10.1017/s0007114510003466 2024-07-03T04:03:41Z The pancreatic enzyme secretory phospholipase A 2 group IB (sPLA 2 IB) hydrolyses phospholipids at the sn-2 position, resulting in a NEFA and a lyso-phospholipid, which are then absorbed by the enterocytes. The sPLA 2 IB is a member of a family of nineteen enzymes sharing the same catalytic ability, of which nine are cytosolic and ten are secretory. Presently, there are no pharmacological tools to separate between the different secretory enzymes when measuring the enzymatic activity. Thus, it is important to support activity data with more precise techniques when isolation of intestinal content is not possible for analysis, as in the case of small teleost larvae, where the whole animal is sometimes analysed. In the present study, we characterise the sPLA 2 IB gene in Atlantic cod ( Gadus morhua ) and describe its ontogeny at the genetic and protein level and compare this to the total sPLA 2 activity level. A positive correlation was found between the expression of sPLA 2 IB mRNA and protein. Both remained stable and low during the larval stage followed by an increase from day 62 posthatch, coinciding with the development of the pyloric ceaca. Meanwhile, total sPLA 2 enzyme activity in cod was stable and relatively high during the early stages when larvae were fed live prey, followed by a decrease in activity when the fish were weaned to a formulated diet. Thus, the expression of sPLA 2 IB mRNA and protein did not correlate with total sPLA 2 activity. Article in Journal/Newspaper atlantic cod Gadus morhua Cambridge University Press British Journal of Nutrition 105 2 228 237 |
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Cambridge University Press |
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language |
English |
description |
The pancreatic enzyme secretory phospholipase A 2 group IB (sPLA 2 IB) hydrolyses phospholipids at the sn-2 position, resulting in a NEFA and a lyso-phospholipid, which are then absorbed by the enterocytes. The sPLA 2 IB is a member of a family of nineteen enzymes sharing the same catalytic ability, of which nine are cytosolic and ten are secretory. Presently, there are no pharmacological tools to separate between the different secretory enzymes when measuring the enzymatic activity. Thus, it is important to support activity data with more precise techniques when isolation of intestinal content is not possible for analysis, as in the case of small teleost larvae, where the whole animal is sometimes analysed. In the present study, we characterise the sPLA 2 IB gene in Atlantic cod ( Gadus morhua ) and describe its ontogeny at the genetic and protein level and compare this to the total sPLA 2 activity level. A positive correlation was found between the expression of sPLA 2 IB mRNA and protein. Both remained stable and low during the larval stage followed by an increase from day 62 posthatch, coinciding with the development of the pyloric ceaca. Meanwhile, total sPLA 2 enzyme activity in cod was stable and relatively high during the early stages when larvae were fed live prey, followed by a decrease in activity when the fish were weaned to a formulated diet. Thus, the expression of sPLA 2 IB mRNA and protein did not correlate with total sPLA 2 activity. |
format |
Article in Journal/Newspaper |
author |
Sæle, Øystein Nordgreen, Andreas Olsvik, Pål A. Hamre, Kristin |
spellingShingle |
Sæle, Øystein Nordgreen, Andreas Olsvik, Pål A. Hamre, Kristin Characterisation and expression of secretory phospholipase A 2 group IB during ontogeny of Atlantic cod ( Gadus morhua) |
author_facet |
Sæle, Øystein Nordgreen, Andreas Olsvik, Pål A. Hamre, Kristin |
author_sort |
Sæle, Øystein |
title |
Characterisation and expression of secretory phospholipase A 2 group IB during ontogeny of Atlantic cod ( Gadus morhua) |
title_short |
Characterisation and expression of secretory phospholipase A 2 group IB during ontogeny of Atlantic cod ( Gadus morhua) |
title_full |
Characterisation and expression of secretory phospholipase A 2 group IB during ontogeny of Atlantic cod ( Gadus morhua) |
title_fullStr |
Characterisation and expression of secretory phospholipase A 2 group IB during ontogeny of Atlantic cod ( Gadus morhua) |
title_full_unstemmed |
Characterisation and expression of secretory phospholipase A 2 group IB during ontogeny of Atlantic cod ( Gadus morhua) |
title_sort |
characterisation and expression of secretory phospholipase a 2 group ib during ontogeny of atlantic cod ( gadus morhua) |
publisher |
Cambridge University Press (CUP) |
publishDate |
2010 |
url |
http://dx.doi.org/10.1017/s0007114510003466 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0007114510003466 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
British Journal of Nutrition volume 105, issue 2, page 228-237 ISSN 0007-1145 1475-2662 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0007114510003466 |
container_title |
British Journal of Nutrition |
container_volume |
105 |
container_issue |
2 |
container_start_page |
228 |
op_container_end_page |
237 |
_version_ |
1810431744387579904 |