Variation in the ribosomal ITS-sequence of the lichens Buellia Frigida and Xanthoria Elegans from the Vestfold Hills, Eastern Antarctica
Abstract Thalli of the lichens Buellia frigida and Xanthoria elegans were collected from five different locations each 5-15 km apart in the Vestfold Hills, Princess Elizabeth Land, eastern Antarctica. A further collection was made from Mawson Station, Mac Robertson Land, eastern Antarctica, 660 km a...
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Cambridge University Press (CUP)
2001
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crcambridgeupr:10.1006/lich.2000.0306 2024-06-09T07:40:15+00:00 Variation in the ribosomal ITS-sequence of the lichens Buellia Frigida and Xanthoria Elegans from the Vestfold Hills, Eastern Antarctica Dyer, P. S. Murtagh, G. J. 2001 http://dx.doi.org/10.1006/lich.2000.0306 http://api.elsevier.com/content/article/PII:S0024-2829(00)90306-2?httpAccept=text/xml http://api.elsevier.com/content/article/PII:S0024-2829(00)90306-2?httpAccept=text/plain https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0024282901000184 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms The Lichenologist volume 33, issue 2, page 151-159 ISSN 0024-2829 1096-1135 journal-article 2001 crcambridgeupr https://doi.org/10.1006/lich.2000.0306 2024-05-15T13:14:14Z Abstract Thalli of the lichens Buellia frigida and Xanthoria elegans were collected from five different locations each 5-15 km apart in the Vestfold Hills, Princess Elizabeth Land, eastern Antarctica. A further collection was made from Mawson Station, Mac Robertson Land, eastern Antarctica, 660 km away. DNA was extracted from whole thalli and the ribosomal ITS region amplified by PCR using fungal specific primers. Resulting products were sequenced to gain an indication of whether or not variation was present within populations of lichen-forming fungi from continental Antarctica, and therefore of the availability of genetic resources to react to pressures such as climate change. Three genotypes of B. frigida and two of X. elegans were detected in the Vestfold Hill collections. However, these differed by only one nucleotide position suggesting the presence of relatively little genetic variation, if the ITS region is indicative of the overall genome. Buellia frigida collected from Mawson Station had an identical ITS region sequence to the most common Vestfold Hills genotype, indicating that this species may have a low level of genetic variation across much of eastern Antarctica. In contrast, X. elegans collected from Mawson showed considerable genetic variation from the Vestfolds thalli, differing at 14·2% of nucleotide positions and had an identical ITS region sequence to an isolate from maritime Antarctica 4960 km away. Samples from the Vestfold Hills formed a distinct cluster in a phylogenetic analysis of ITS sequences from a worldwide collection of X. elegans isolates. Article in Journal/Newspaper Antarc* Antarctica Mac Robertson Land Mac. Robertson Land Princess Elizabeth Land Cambridge University Press Mac. Robertson Land ENVELOPE(65.000,65.000,-70.000,-70.000) Mawson Station ENVELOPE(62.874,62.874,-67.603,-67.603) Princess Elizabeth Land ENVELOPE(80.367,80.367,-68.500,-68.500) Vestfold Vestfold Hills The Lichenologist 33 2 151 159 |
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Open Polar |
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Cambridge University Press |
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crcambridgeupr |
language |
English |
description |
Abstract Thalli of the lichens Buellia frigida and Xanthoria elegans were collected from five different locations each 5-15 km apart in the Vestfold Hills, Princess Elizabeth Land, eastern Antarctica. A further collection was made from Mawson Station, Mac Robertson Land, eastern Antarctica, 660 km away. DNA was extracted from whole thalli and the ribosomal ITS region amplified by PCR using fungal specific primers. Resulting products were sequenced to gain an indication of whether or not variation was present within populations of lichen-forming fungi from continental Antarctica, and therefore of the availability of genetic resources to react to pressures such as climate change. Three genotypes of B. frigida and two of X. elegans were detected in the Vestfold Hill collections. However, these differed by only one nucleotide position suggesting the presence of relatively little genetic variation, if the ITS region is indicative of the overall genome. Buellia frigida collected from Mawson Station had an identical ITS region sequence to the most common Vestfold Hills genotype, indicating that this species may have a low level of genetic variation across much of eastern Antarctica. In contrast, X. elegans collected from Mawson showed considerable genetic variation from the Vestfolds thalli, differing at 14·2% of nucleotide positions and had an identical ITS region sequence to an isolate from maritime Antarctica 4960 km away. Samples from the Vestfold Hills formed a distinct cluster in a phylogenetic analysis of ITS sequences from a worldwide collection of X. elegans isolates. |
format |
Article in Journal/Newspaper |
author |
Dyer, P. S. Murtagh, G. J. |
spellingShingle |
Dyer, P. S. Murtagh, G. J. Variation in the ribosomal ITS-sequence of the lichens Buellia Frigida and Xanthoria Elegans from the Vestfold Hills, Eastern Antarctica |
author_facet |
Dyer, P. S. Murtagh, G. J. |
author_sort |
Dyer, P. S. |
title |
Variation in the ribosomal ITS-sequence of the lichens Buellia Frigida and Xanthoria Elegans from the Vestfold Hills, Eastern Antarctica |
title_short |
Variation in the ribosomal ITS-sequence of the lichens Buellia Frigida and Xanthoria Elegans from the Vestfold Hills, Eastern Antarctica |
title_full |
Variation in the ribosomal ITS-sequence of the lichens Buellia Frigida and Xanthoria Elegans from the Vestfold Hills, Eastern Antarctica |
title_fullStr |
Variation in the ribosomal ITS-sequence of the lichens Buellia Frigida and Xanthoria Elegans from the Vestfold Hills, Eastern Antarctica |
title_full_unstemmed |
Variation in the ribosomal ITS-sequence of the lichens Buellia Frigida and Xanthoria Elegans from the Vestfold Hills, Eastern Antarctica |
title_sort |
variation in the ribosomal its-sequence of the lichens buellia frigida and xanthoria elegans from the vestfold hills, eastern antarctica |
publisher |
Cambridge University Press (CUP) |
publishDate |
2001 |
url |
http://dx.doi.org/10.1006/lich.2000.0306 http://api.elsevier.com/content/article/PII:S0024-2829(00)90306-2?httpAccept=text/xml http://api.elsevier.com/content/article/PII:S0024-2829(00)90306-2?httpAccept=text/plain https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0024282901000184 |
long_lat |
ENVELOPE(65.000,65.000,-70.000,-70.000) ENVELOPE(62.874,62.874,-67.603,-67.603) ENVELOPE(80.367,80.367,-68.500,-68.500) |
geographic |
Mac. Robertson Land Mawson Station Princess Elizabeth Land Vestfold Vestfold Hills |
geographic_facet |
Mac. Robertson Land Mawson Station Princess Elizabeth Land Vestfold Vestfold Hills |
genre |
Antarc* Antarctica Mac Robertson Land Mac. Robertson Land Princess Elizabeth Land |
genre_facet |
Antarc* Antarctica Mac Robertson Land Mac. Robertson Land Princess Elizabeth Land |
op_source |
The Lichenologist volume 33, issue 2, page 151-159 ISSN 0024-2829 1096-1135 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1006/lich.2000.0306 |
container_title |
The Lichenologist |
container_volume |
33 |
container_issue |
2 |
container_start_page |
151 |
op_container_end_page |
159 |
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1801383700444741632 |