The integration of cloning by nuclear transfer in the conservation of animal genetic resources

• Published in: BSAP Occasional Publication
• Main Author: Wells, D.N.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Cambridge University Press (CUP) 2004
• Subjects: Enderby Island
• Online Access: https://doi.org/10.1017/s0263967x0004204x; https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0263967X0004204X

Abstract Cloning mammals from somatic cells by nuclear transfer has the potential to assist with the preservation of genetic diversity. An increasing number of species have been successfully cloned by this approach; however, present methods are inefficient with few cloned embryos resulting in healthy offspring. In those livestock species that have already been cloned, it is clearly feasible to use cloning to preserve endangered breeds (e.g. the last surviving Enderby Island cow). The opportunity exists to recover oocytes from these cloned heifers and use frozen Enderby Island sperm from deceased bulls for in vitro fertilisation and thus, expand the genetic diversity of this breed. Where there exists an adequate understanding of the reproductive biology and embryology of the species concerned and adequate sources of females to supply both recipient oocytes and surrogates to gestate the pregnancies, intra-specific nuclear transfer and embryo transfer can be utilised. However, when these requirements cannot be met, as is common for most endangered species, cloning technology invariably involves the use of inter-species nuclear transfer and embryo transfer. Even in intra-specific cloning the source of oocyte for nuclear transfer is an important consideration. Typically, cloned animals are only genomic copies of the founder if they possess mitochondrial DNA which differs from the original animal. Different maternal lineages of oocytes both within and between breeds significantly affect cloning efficiency and livestock production characteristics. Cloning should not distract conservation efforts from encouraging the use of indigenous livestock breeds with traits of adaptation to local environments, the preservation of wildlife habitats or the use of other forms of assisted reproduction. Whilst it is often difficult to justify cloning in animal conservation at present, the appropriate cryo-preservation of tissues and cells from a wide selection of biodiversity is of paramount importance. This provides an insurance ...