Lipofuscin Quantification as a Potential Tool for Age Estimation in Snow Crabs,Chionoecetes opilio (O. Fabricius, 1788) (Decapoda, Oregoniidae)
Abstract Lipofuscin levels were investigated in snow crabs (Chionoecetes opilio) to assess the applicability of lipofuscin quantification as an age determining tool for this species. Localization and quantification of lipofuscin granules in the olfactory lobe cell mass (OLCM) of the brain were condu...
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crbrillap:10.1163/156854011x605729 2023-07-02T03:31:57+02:00 Lipofuscin Quantification as a Potential Tool for Age Estimation in Snow Crabs,Chionoecetes opilio (O. Fabricius, 1788) (Decapoda, Oregoniidae) Allain, R. N. Moriyasu, M. Crawford, B. D. Courtenay, S. C. 2011 http://dx.doi.org/10.1163/156854011x605729 https://brill.com/view/journals/cr/84/12-13/article-p1441_3.xml https://brill.com/downloadpdf/journals/cr/84/12-13/article-p1441_3.xml unknown Brill Crustaceana volume 84, issue 12-13, page 1441-1463 ISSN 0011-216X 1568-5403 Animal Science and Zoology Aquatic Science journal-article 2011 crbrillap https://doi.org/10.1163/156854011x605729 2023-06-09T14:45:07Z Abstract Lipofuscin levels were investigated in snow crabs (Chionoecetes opilio) to assess the applicability of lipofuscin quantification as an age determining tool for this species. Localization and quantification of lipofuscin granules in the olfactory lobe cell mass (OLCM) of the brain were conducted by confocal microscopy and image analysis. The majority of immature crabs had no detectable lipofuscin. Crabs with a dirty carapace condition (an indication of greater age) had more lipofuscin (in terms of granule density, area fraction, and mean granule size) than crabs with a clean carapace condition; however, the amount of lipofuscin was much lower than that in more temperate species. Lipofuscin content was positively correlated with carapace size among crabs with a clean carapace but not among crabs with a dirty carapace. No correlation was observed between average lipofuscin granule fluorescent intensity and carapace width. OLCM neuron characteristics were also investigated. An inverse relationship was observed between neuron density and carapace width although it is unknown if this density reduction is due to a loss of neurons, an increase in size of the structures, or both. Furthermore, a positive relationship was observed between neuron size and carapace width. The range of neuron size also increased in larger crabs, suggesting the presence of mitotically active cells in the OLCM. Although lipofuscin levels were higher in dirty carapace condition (presumably older) crabs than in clean carapace crabs and increased with size (in mature, clean carapace snow crabs), the lack of detectable lipofuscin in most immature crabs, generally low levels of lipofuscin in mature crabs, and lack of increase with size in older (dirty carapace) crabs limits the applicability of this age estimation tool for this species. Article in Journal/Newspaper Chionoecetes opilio Brill (via Crossref) Crustaceana 84 12-13 1441 1463 |
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Brill (via Crossref) |
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crbrillap |
language |
unknown |
topic |
Animal Science and Zoology Aquatic Science |
spellingShingle |
Animal Science and Zoology Aquatic Science Allain, R. N. Moriyasu, M. Crawford, B. D. Courtenay, S. C. Lipofuscin Quantification as a Potential Tool for Age Estimation in Snow Crabs,Chionoecetes opilio (O. Fabricius, 1788) (Decapoda, Oregoniidae) |
topic_facet |
Animal Science and Zoology Aquatic Science |
description |
Abstract Lipofuscin levels were investigated in snow crabs (Chionoecetes opilio) to assess the applicability of lipofuscin quantification as an age determining tool for this species. Localization and quantification of lipofuscin granules in the olfactory lobe cell mass (OLCM) of the brain were conducted by confocal microscopy and image analysis. The majority of immature crabs had no detectable lipofuscin. Crabs with a dirty carapace condition (an indication of greater age) had more lipofuscin (in terms of granule density, area fraction, and mean granule size) than crabs with a clean carapace condition; however, the amount of lipofuscin was much lower than that in more temperate species. Lipofuscin content was positively correlated with carapace size among crabs with a clean carapace but not among crabs with a dirty carapace. No correlation was observed between average lipofuscin granule fluorescent intensity and carapace width. OLCM neuron characteristics were also investigated. An inverse relationship was observed between neuron density and carapace width although it is unknown if this density reduction is due to a loss of neurons, an increase in size of the structures, or both. Furthermore, a positive relationship was observed between neuron size and carapace width. The range of neuron size also increased in larger crabs, suggesting the presence of mitotically active cells in the OLCM. Although lipofuscin levels were higher in dirty carapace condition (presumably older) crabs than in clean carapace crabs and increased with size (in mature, clean carapace snow crabs), the lack of detectable lipofuscin in most immature crabs, generally low levels of lipofuscin in mature crabs, and lack of increase with size in older (dirty carapace) crabs limits the applicability of this age estimation tool for this species. |
format |
Article in Journal/Newspaper |
author |
Allain, R. N. Moriyasu, M. Crawford, B. D. Courtenay, S. C. |
author_facet |
Allain, R. N. Moriyasu, M. Crawford, B. D. Courtenay, S. C. |
author_sort |
Allain, R. N. |
title |
Lipofuscin Quantification as a Potential Tool for Age Estimation in Snow Crabs,Chionoecetes opilio (O. Fabricius, 1788) (Decapoda, Oregoniidae) |
title_short |
Lipofuscin Quantification as a Potential Tool for Age Estimation in Snow Crabs,Chionoecetes opilio (O. Fabricius, 1788) (Decapoda, Oregoniidae) |
title_full |
Lipofuscin Quantification as a Potential Tool for Age Estimation in Snow Crabs,Chionoecetes opilio (O. Fabricius, 1788) (Decapoda, Oregoniidae) |
title_fullStr |
Lipofuscin Quantification as a Potential Tool for Age Estimation in Snow Crabs,Chionoecetes opilio (O. Fabricius, 1788) (Decapoda, Oregoniidae) |
title_full_unstemmed |
Lipofuscin Quantification as a Potential Tool for Age Estimation in Snow Crabs,Chionoecetes opilio (O. Fabricius, 1788) (Decapoda, Oregoniidae) |
title_sort |
lipofuscin quantification as a potential tool for age estimation in snow crabs,chionoecetes opilio (o. fabricius, 1788) (decapoda, oregoniidae) |
publisher |
Brill |
publishDate |
2011 |
url |
http://dx.doi.org/10.1163/156854011x605729 https://brill.com/view/journals/cr/84/12-13/article-p1441_3.xml https://brill.com/downloadpdf/journals/cr/84/12-13/article-p1441_3.xml |
genre |
Chionoecetes opilio |
genre_facet |
Chionoecetes opilio |
op_source |
Crustaceana volume 84, issue 12-13, page 1441-1463 ISSN 0011-216X 1568-5403 |
op_doi |
https://doi.org/10.1163/156854011x605729 |
container_title |
Crustaceana |
container_volume |
84 |
container_issue |
12-13 |
container_start_page |
1441 |
op_container_end_page |
1463 |
_version_ |
1770271413150679040 |