Molecular cloning and differential expression of three GnRH mRNAs in discrete brain areas and lymphocytes in red drum
Three gonadotropin releasing hormones, seabream GnRH (GnRH-I), chicken GnRH-II (GnRH-II) and salmon GnRH (GnRH-III) cDNAs were isolated from the brain of the red drum, Sciaenops ocellatus. The GnRH cDNA sequences of red drum showed more similarity to those of Atlantic croaker, sea bass and sea bream...
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crbioscientif:10.1677/joe.1.06423 2023-11-12T04:25:13+01:00 Molecular cloning and differential expression of three GnRH mRNAs in discrete brain areas and lymphocytes in red drum Mohamed, J Shaik Khan, I A 2006 http://dx.doi.org/10.1677/joe.1.06423 https://joe.bioscientifica.com/view/journals/joe/188/3/1880407.xml https://joe.bioscientifica.com/downloadpdf/journals/joe/188/3/1880407.xml unknown Bioscientifica Journal of Endocrinology volume 188, issue 3, page 407-416 ISSN 0022-0795 1479-6805 Endocrinology Endocrinology, Diabetes and Metabolism journal-article 2006 crbioscientif https://doi.org/10.1677/joe.1.06423 2023-10-20T08:25:26Z Three gonadotropin releasing hormones, seabream GnRH (GnRH-I), chicken GnRH-II (GnRH-II) and salmon GnRH (GnRH-III) cDNAs were isolated from the brain of the red drum, Sciaenops ocellatus. The GnRH cDNA sequences of red drum showed more similarity to those of Atlantic croaker, sea bass and sea bream. The real-time quantitative RT-PCR study revealed expression of GnRH-I and GnRH III mRNAs in the olfactory bulb plus telencephalon (OB+TEL), and preoptic-anterior hypothalamic area (POAH), indicating an overlap of the GnRH-I and GnRH-III systems in these forebrain regions. However, GnRH-II mRNA expression was detected only in the midbrain tegmentum (MT). The GnRH-I mRNA expression in the POAH in fish undergoing gonadal recrudescence was significantly higher than that in gonadally immature individuals, suggesting involvement of the POAH GnRH-I in gonadal maturation. On the other hand, GnRH-III mRNA expression was significantly higher in the OB+TEL region compared with that in the POAH. Moreover, the demonstration of GnRH-I mRNA and peptide expression in red drum lymphocytes indicates that the GnRH-I is synthesized in these cells of the immune system similar to the situation in mammals. Article in Journal/Newspaper Red drum Sciaenops ocellatus Bioscientifica (via Crossref) Journal of Endocrinology 188 3 407 416 |
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Bioscientifica (via Crossref) |
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Endocrinology Endocrinology, Diabetes and Metabolism |
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Endocrinology Endocrinology, Diabetes and Metabolism Mohamed, J Shaik Khan, I A Molecular cloning and differential expression of three GnRH mRNAs in discrete brain areas and lymphocytes in red drum |
topic_facet |
Endocrinology Endocrinology, Diabetes and Metabolism |
description |
Three gonadotropin releasing hormones, seabream GnRH (GnRH-I), chicken GnRH-II (GnRH-II) and salmon GnRH (GnRH-III) cDNAs were isolated from the brain of the red drum, Sciaenops ocellatus. The GnRH cDNA sequences of red drum showed more similarity to those of Atlantic croaker, sea bass and sea bream. The real-time quantitative RT-PCR study revealed expression of GnRH-I and GnRH III mRNAs in the olfactory bulb plus telencephalon (OB+TEL), and preoptic-anterior hypothalamic area (POAH), indicating an overlap of the GnRH-I and GnRH-III systems in these forebrain regions. However, GnRH-II mRNA expression was detected only in the midbrain tegmentum (MT). The GnRH-I mRNA expression in the POAH in fish undergoing gonadal recrudescence was significantly higher than that in gonadally immature individuals, suggesting involvement of the POAH GnRH-I in gonadal maturation. On the other hand, GnRH-III mRNA expression was significantly higher in the OB+TEL region compared with that in the POAH. Moreover, the demonstration of GnRH-I mRNA and peptide expression in red drum lymphocytes indicates that the GnRH-I is synthesized in these cells of the immune system similar to the situation in mammals. |
format |
Article in Journal/Newspaper |
author |
Mohamed, J Shaik Khan, I A |
author_facet |
Mohamed, J Shaik Khan, I A |
author_sort |
Mohamed, J Shaik |
title |
Molecular cloning and differential expression of three GnRH mRNAs in discrete brain areas and lymphocytes in red drum |
title_short |
Molecular cloning and differential expression of three GnRH mRNAs in discrete brain areas and lymphocytes in red drum |
title_full |
Molecular cloning and differential expression of three GnRH mRNAs in discrete brain areas and lymphocytes in red drum |
title_fullStr |
Molecular cloning and differential expression of three GnRH mRNAs in discrete brain areas and lymphocytes in red drum |
title_full_unstemmed |
Molecular cloning and differential expression of three GnRH mRNAs in discrete brain areas and lymphocytes in red drum |
title_sort |
molecular cloning and differential expression of three gnrh mrnas in discrete brain areas and lymphocytes in red drum |
publisher |
Bioscientifica |
publishDate |
2006 |
url |
http://dx.doi.org/10.1677/joe.1.06423 https://joe.bioscientifica.com/view/journals/joe/188/3/1880407.xml https://joe.bioscientifica.com/downloadpdf/journals/joe/188/3/1880407.xml |
genre |
Red drum Sciaenops ocellatus |
genre_facet |
Red drum Sciaenops ocellatus |
op_source |
Journal of Endocrinology volume 188, issue 3, page 407-416 ISSN 0022-0795 1479-6805 |
op_doi |
https://doi.org/10.1677/joe.1.06423 |
container_title |
Journal of Endocrinology |
container_volume |
188 |
container_issue |
3 |
container_start_page |
407 |
op_container_end_page |
416 |
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1782339542944055296 |