Molecular cloning, identification and characterization of four distinct receptor subtypes for insulin and IGF-I in Japanese flounder, Paralichthys olivaceus
Insulin receptor (IR) and IGF-I receptor (IGF-IR) are structurally and functionally related and belong to the tyrosine kinase receptor family. In teleosti such as salmonids and turbot, occurrence of multiple IR and IGF-IR members has been reported, but the structures of a complete set of both IR and...
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crbioscientif:10.1677/joe.0.1730365 2023-10-09T21:56:21+02:00 Molecular cloning, identification and characterization of four distinct receptor subtypes for insulin and IGF-I in Japanese flounder, Paralichthys olivaceus Nakao, N Tanaka, M Higashimoto, Y Nakashima, K 2002 http://dx.doi.org/10.1677/joe.0.1730365 https://joe.bioscientifica.com/view/journals/joe/173/2/365.xml https://joe.bioscientifica.com/downloadpdf/journals/joe/173/2/365.xml unknown Bioscientifica Journal of Endocrinology volume 173, issue 2, page 365-375 ISSN 0022-0795 1479-6805 Endocrinology Endocrinology, Diabetes and Metabolism journal-article 2002 crbioscientif https://doi.org/10.1677/joe.0.1730365 2023-09-15T07:14:45Z Insulin receptor (IR) and IGF-I receptor (IGF-IR) are structurally and functionally related and belong to the tyrosine kinase receptor family. In teleosti such as salmonids and turbot, occurrence of multiple IR and IGF-IR members has been reported, but the structures of a complete set of both IR and IGF-IR members in a single teleost species have not yet been characterized. In this study, we cloned and analysed four distinct cDNA clones for IR and IGF-IR members from the liver and kidney of the Japanese flounder (Paralichthys olivaceus). Deduced amino acid sequence analyses and phylogenetic analysis have revealed that two of them (fIR-1 and fIR-2) belong to IR members and the other two (fIGF-IR-1 and fIGF-IR-2) are IGF-IRs. fIR-1 and fIR-2 comprised 1369 and 1368 amino acid residues respectively, and fIGF-IR-1 and fIGF-IR-2 comprised 1412 and 1418 residues respectively. All the receptor proteins contained cysteine-rich domains in their alpha-subunits, and conserved each transmembrane and tyrosine kinase domains in their beta-subunits. The amino acid sequences of fIRs and fIGF-IRs showed more than 90% sequence identity with turbot IR and IGF-IR respectively. When compared with their mammalian homologues, fIGF-IR-1 and fIGF-IR-2 proteins contained large insertions at their C-termini, as was observed in the corresponding region of turbot IGF-IR. Occurrence of multiple species of mRNA for each IR and IGF-IR was suggested by Northern blot analyses. A ribonuclease protection assay revealed diverse expressions of four receptor mRNAs in a wide range of tissues including heart, liver, ovary, testis, brain, gill arch, kidney, skeletal muscle, intestine, stomach, spleen and eye of the flounder. Article in Journal/Newspaper Turbot Bioscientifica (via Crossref) Journal of Endocrinology 173 2 365 375 |
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Open Polar |
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Bioscientifica (via Crossref) |
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Endocrinology Endocrinology, Diabetes and Metabolism |
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Endocrinology Endocrinology, Diabetes and Metabolism Nakao, N Tanaka, M Higashimoto, Y Nakashima, K Molecular cloning, identification and characterization of four distinct receptor subtypes for insulin and IGF-I in Japanese flounder, Paralichthys olivaceus |
topic_facet |
Endocrinology Endocrinology, Diabetes and Metabolism |
description |
Insulin receptor (IR) and IGF-I receptor (IGF-IR) are structurally and functionally related and belong to the tyrosine kinase receptor family. In teleosti such as salmonids and turbot, occurrence of multiple IR and IGF-IR members has been reported, but the structures of a complete set of both IR and IGF-IR members in a single teleost species have not yet been characterized. In this study, we cloned and analysed four distinct cDNA clones for IR and IGF-IR members from the liver and kidney of the Japanese flounder (Paralichthys olivaceus). Deduced amino acid sequence analyses and phylogenetic analysis have revealed that two of them (fIR-1 and fIR-2) belong to IR members and the other two (fIGF-IR-1 and fIGF-IR-2) are IGF-IRs. fIR-1 and fIR-2 comprised 1369 and 1368 amino acid residues respectively, and fIGF-IR-1 and fIGF-IR-2 comprised 1412 and 1418 residues respectively. All the receptor proteins contained cysteine-rich domains in their alpha-subunits, and conserved each transmembrane and tyrosine kinase domains in their beta-subunits. The amino acid sequences of fIRs and fIGF-IRs showed more than 90% sequence identity with turbot IR and IGF-IR respectively. When compared with their mammalian homologues, fIGF-IR-1 and fIGF-IR-2 proteins contained large insertions at their C-termini, as was observed in the corresponding region of turbot IGF-IR. Occurrence of multiple species of mRNA for each IR and IGF-IR was suggested by Northern blot analyses. A ribonuclease protection assay revealed diverse expressions of four receptor mRNAs in a wide range of tissues including heart, liver, ovary, testis, brain, gill arch, kidney, skeletal muscle, intestine, stomach, spleen and eye of the flounder. |
format |
Article in Journal/Newspaper |
author |
Nakao, N Tanaka, M Higashimoto, Y Nakashima, K |
author_facet |
Nakao, N Tanaka, M Higashimoto, Y Nakashima, K |
author_sort |
Nakao, N |
title |
Molecular cloning, identification and characterization of four distinct receptor subtypes for insulin and IGF-I in Japanese flounder, Paralichthys olivaceus |
title_short |
Molecular cloning, identification and characterization of four distinct receptor subtypes for insulin and IGF-I in Japanese flounder, Paralichthys olivaceus |
title_full |
Molecular cloning, identification and characterization of four distinct receptor subtypes for insulin and IGF-I in Japanese flounder, Paralichthys olivaceus |
title_fullStr |
Molecular cloning, identification and characterization of four distinct receptor subtypes for insulin and IGF-I in Japanese flounder, Paralichthys olivaceus |
title_full_unstemmed |
Molecular cloning, identification and characterization of four distinct receptor subtypes for insulin and IGF-I in Japanese flounder, Paralichthys olivaceus |
title_sort |
molecular cloning, identification and characterization of four distinct receptor subtypes for insulin and igf-i in japanese flounder, paralichthys olivaceus |
publisher |
Bioscientifica |
publishDate |
2002 |
url |
http://dx.doi.org/10.1677/joe.0.1730365 https://joe.bioscientifica.com/view/journals/joe/173/2/365.xml https://joe.bioscientifica.com/downloadpdf/journals/joe/173/2/365.xml |
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Turbot |
genre_facet |
Turbot |
op_source |
Journal of Endocrinology volume 173, issue 2, page 365-375 ISSN 0022-0795 1479-6805 |
op_doi |
https://doi.org/10.1677/joe.0.1730365 |
container_title |
Journal of Endocrinology |
container_volume |
173 |
container_issue |
2 |
container_start_page |
365 |
op_container_end_page |
375 |
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1779321024345538560 |