BIOGENESIS OF CORTICOSTEROIDS IN THE EUROPEAN EEL ANGUILLA ANGUILLA L.

• Published in: Journal of Endocrinology
• Main Authors: SANDOR, T., VINSON, G. P., JONES, I. CHESTER, HENDERSON, I. W., WHITEHOUSE, B. J.
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Bioscientifica 1966
• Subjects: Endocrinology; Endocrinology, Diabetes and Metabolism; Anguilla anguilla
• Online Access: http://dx.doi.org/10.1677/joe.0.0340105; https://joe.bioscientifica.com/view/journals/joe/34/1/joe_34_1_011.xml; https://joe.bioscientifica.com/downloadpdf/journals/joe/34/1/joe_34_1_011.xml

SUMMARY Incubation, with [4- 14 C]progesterone, of adrenocortical tissue (posterior cardinal vein preparations) from the eel yielded 14 C-labelled cortisol and cortisone. These two hormones, containing both 14 C and 3 H, appeared when similar preparations were incubated simultaneously with [4- 14 C]progesterone and [16- 3 H]pregnenolone. In addition, the following intermediaries were isolated: [ 14 C, 3 H]progesterone; [ 14 C, 3 H]17α-hydroxyprogesterone; [ 3 H]17α-hydroxypregnenolone and [ 14 C, 3 H]21-deoxycortisol. Analysis of the isotope content of the end products and isolated intermediaries seemed to indicate that the transformation of pregnenolone to corticosteroids proceeded to a large extent through intermediaries other than progesterone. In none of these experiments could the formation of aldosterone be demonstrated. Similarly, incubation of eel adrenocortical tissue with a mixture of [4- 14 C]-progesterone and [1,2- 3 H]corticosterone failed to yield detectable aldosterone. A further search for aldosterone, using a large amount of eel adrenocortical tissue with [4- 14 C]progesterone and [16- 3 H]pregnenolone as substrates with added angiotensin, also gave negative results. In similar preparations, [1- 14 C]sodium acetate was not transformed to any recognizable corticosteroids.