Cortisol regulation of ion transporter mRNA in Atlantic salmon gill and the effect of salinity on the signaling pathway
Based on real-time RT-PCR, analysis of transcripts of selected ion-regulatory proteins (Na + , K + -ATPase α1a and α1b subunit, Na + , K + , 2Cl − cotransporter, cystic fibrosis transmembrane conductance regulator (CFTR), and H + -ATPase B-subunit), the regulatory role of cortisol and the associated...
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crbioscientif:10.1677/joe-07-0185 2024-06-23T07:51:25+00:00 Cortisol regulation of ion transporter mRNA in Atlantic salmon gill and the effect of salinity on the signaling pathway Kiilerich, Pia Kristiansen, Karsten Madsen, Steffen S 2007 http://dx.doi.org/10.1677/joe-07-0185 https://joe.bioscientifica.com/view/journals/joe/194/2/1940417.xml https://joe.bioscientifica.com/downloadpdf/journals/joe/194/2/1940417.xml unknown Bioscientifica Journal of Endocrinology volume 194, issue 2, page 417-427 ISSN 0022-0795 1479-6805 journal-article 2007 crbioscientif https://doi.org/10.1677/joe-07-0185 2024-06-06T04:11:07Z Based on real-time RT-PCR, analysis of transcripts of selected ion-regulatory proteins (Na + , K + -ATPase α1a and α1b subunit, Na + , K + , 2Cl − cotransporter, cystic fibrosis transmembrane conductance regulator (CFTR), and H + -ATPase B-subunit), the regulatory role of cortisol and the associated receptor signaling pathway (glucocorticoid (GR) versus mineralocorticoid (MR)) of cortisol was investigated in the salmon gill. Using a gill organ culture technique, the effect of cortisol with and without addition of specific hormone receptor antagonists (RU486 and spironolactone) was analyzed in gills from freshwater (FW) and seawater (SW) acclimated fish. The effect of cortisol was highly dependent on acclimation to salinity. In FW, cortisol stimulated the transcript levels of CFTR-I and Na + , K + -ATPase α1a and α1b. Addition of RU486 totally abolished the cortisol effects on CFTR-I and Na + , K + -ATPase α1b, suggesting that signaling was mediated via GR. Interestingly, both spironolactone and RU486 were able to inhibit the cortisol effect on Na + , K + -ATPase α1a indicating a role for both MR and GR in regulation of this target gene. In SW, cortisol increased the transcript levels of CFTR-I, CFTR-II, Na + , K + -ATPase α1a and α1b, and NKCC. Interestingly, the effect of cortisol on CFTR-I and Na + , K + -ATPase α1a was mediated through GR and MR respectively, while both GR and MR signaling were required in the regulation of CFTR-II and Na + , K + -ATPase α1b. In FW gills, GR1 and MR transcript levels were not significantly affected by cortisol. In SW gills, GR1 and MR transcripts were downregulated by cortisol; GR1 was regulated via the MR and MR regulation was mediated via GR. Article in Journal/Newspaper Atlantic salmon Bioscientifica Journal of Endocrinology 194 2 417 427 |
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Open Polar |
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Bioscientifica |
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crbioscientif |
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description |
Based on real-time RT-PCR, analysis of transcripts of selected ion-regulatory proteins (Na + , K + -ATPase α1a and α1b subunit, Na + , K + , 2Cl − cotransporter, cystic fibrosis transmembrane conductance regulator (CFTR), and H + -ATPase B-subunit), the regulatory role of cortisol and the associated receptor signaling pathway (glucocorticoid (GR) versus mineralocorticoid (MR)) of cortisol was investigated in the salmon gill. Using a gill organ culture technique, the effect of cortisol with and without addition of specific hormone receptor antagonists (RU486 and spironolactone) was analyzed in gills from freshwater (FW) and seawater (SW) acclimated fish. The effect of cortisol was highly dependent on acclimation to salinity. In FW, cortisol stimulated the transcript levels of CFTR-I and Na + , K + -ATPase α1a and α1b. Addition of RU486 totally abolished the cortisol effects on CFTR-I and Na + , K + -ATPase α1b, suggesting that signaling was mediated via GR. Interestingly, both spironolactone and RU486 were able to inhibit the cortisol effect on Na + , K + -ATPase α1a indicating a role for both MR and GR in regulation of this target gene. In SW, cortisol increased the transcript levels of CFTR-I, CFTR-II, Na + , K + -ATPase α1a and α1b, and NKCC. Interestingly, the effect of cortisol on CFTR-I and Na + , K + -ATPase α1a was mediated through GR and MR respectively, while both GR and MR signaling were required in the regulation of CFTR-II and Na + , K + -ATPase α1b. In FW gills, GR1 and MR transcript levels were not significantly affected by cortisol. In SW gills, GR1 and MR transcripts were downregulated by cortisol; GR1 was regulated via the MR and MR regulation was mediated via GR. |
format |
Article in Journal/Newspaper |
author |
Kiilerich, Pia Kristiansen, Karsten Madsen, Steffen S |
spellingShingle |
Kiilerich, Pia Kristiansen, Karsten Madsen, Steffen S Cortisol regulation of ion transporter mRNA in Atlantic salmon gill and the effect of salinity on the signaling pathway |
author_facet |
Kiilerich, Pia Kristiansen, Karsten Madsen, Steffen S |
author_sort |
Kiilerich, Pia |
title |
Cortisol regulation of ion transporter mRNA in Atlantic salmon gill and the effect of salinity on the signaling pathway |
title_short |
Cortisol regulation of ion transporter mRNA in Atlantic salmon gill and the effect of salinity on the signaling pathway |
title_full |
Cortisol regulation of ion transporter mRNA in Atlantic salmon gill and the effect of salinity on the signaling pathway |
title_fullStr |
Cortisol regulation of ion transporter mRNA in Atlantic salmon gill and the effect of salinity on the signaling pathway |
title_full_unstemmed |
Cortisol regulation of ion transporter mRNA in Atlantic salmon gill and the effect of salinity on the signaling pathway |
title_sort |
cortisol regulation of ion transporter mrna in atlantic salmon gill and the effect of salinity on the signaling pathway |
publisher |
Bioscientifica |
publishDate |
2007 |
url |
http://dx.doi.org/10.1677/joe-07-0185 https://joe.bioscientifica.com/view/journals/joe/194/2/1940417.xml https://joe.bioscientifica.com/downloadpdf/journals/joe/194/2/1940417.xml |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Journal of Endocrinology volume 194, issue 2, page 417-427 ISSN 0022-0795 1479-6805 |
op_doi |
https://doi.org/10.1677/joe-07-0185 |
container_title |
Journal of Endocrinology |
container_volume |
194 |
container_issue |
2 |
container_start_page |
417 |
op_container_end_page |
427 |
_version_ |
1802642517445836800 |