Molecular cloning and characterization of thyroid hormone receptors in teleost fish
Thyroid hormones are pleiotropic factors important for many developmental and physiological functions in vertebrates. Their effects are mediated by two specific receptors (TRalpha and TRbeta) which are members of the nuclear hormone receptor superfamily. To clarify the function of these receptors, o...
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crbioscientif:10.1677/jme.0.0260051 2024-04-28T08:13:40+00:00 Molecular cloning and characterization of thyroid hormone receptors in teleost fish Marchand, O Safi, R Escriva, H Van Rompaey, E Prunet, P Laudet, V 2001 http://dx.doi.org/10.1677/jme.0.0260051 https://jme.bioscientifica.com/view/journals/jme/26/1/51.xml https://jme.bioscientifica.com/downloadpdf/journals/jme/26/1/51.xml unknown Bioscientifica Journal of Molecular Endocrinology volume 26, issue 1, page 51-65 ISSN 0952-5041 1479-6813 Endocrinology Molecular Biology journal-article 2001 crbioscientif https://doi.org/10.1677/jme.0.0260051 2024-04-02T06:51:25Z Thyroid hormones are pleiotropic factors important for many developmental and physiological functions in vertebrates. Their effects are mediated by two specific receptors (TRalpha and TRbeta) which are members of the nuclear hormone receptor superfamily. To clarify the function of these receptors, our laboratory has started a comparative study of their role in teleost fish. This type of approach has been hampered by the isolation of specific clones for each fish species studied. In this report, we describe an efficient reverse transcription/PCR procedure that allows the isolation of large fragments corresponding to TRalpha and TRbeta of a wide range of teleost fish. Phylogenetic analysis of these receptors revealed a placement consistent with their origin, sequences from teleost fish being clearly monophyletic for both TRalpha and TRbeta. Interestingly, this approach allowed us to isolate (from tilapia and salmon) several new TRalpha or TRbeta isoforms resulting from alternative splicing. These isoforms correspond to expressed transcripts and thus may have an important physiological function. In addition, we isolated a cDNA encoding TRbeta in the Atlantic salmon (Salmo salar) encoding a functional thyroid hormone receptor which binds specific thyroid hormone response elements and regulates transcription in response to thyroid hormones. Article in Journal/Newspaper Atlantic salmon Salmo salar Bioscientifica Journal of Molecular Endocrinology 26 1 51 65 |
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Bioscientifica |
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Endocrinology Molecular Biology |
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Endocrinology Molecular Biology Marchand, O Safi, R Escriva, H Van Rompaey, E Prunet, P Laudet, V Molecular cloning and characterization of thyroid hormone receptors in teleost fish |
topic_facet |
Endocrinology Molecular Biology |
description |
Thyroid hormones are pleiotropic factors important for many developmental and physiological functions in vertebrates. Their effects are mediated by two specific receptors (TRalpha and TRbeta) which are members of the nuclear hormone receptor superfamily. To clarify the function of these receptors, our laboratory has started a comparative study of their role in teleost fish. This type of approach has been hampered by the isolation of specific clones for each fish species studied. In this report, we describe an efficient reverse transcription/PCR procedure that allows the isolation of large fragments corresponding to TRalpha and TRbeta of a wide range of teleost fish. Phylogenetic analysis of these receptors revealed a placement consistent with their origin, sequences from teleost fish being clearly monophyletic for both TRalpha and TRbeta. Interestingly, this approach allowed us to isolate (from tilapia and salmon) several new TRalpha or TRbeta isoforms resulting from alternative splicing. These isoforms correspond to expressed transcripts and thus may have an important physiological function. In addition, we isolated a cDNA encoding TRbeta in the Atlantic salmon (Salmo salar) encoding a functional thyroid hormone receptor which binds specific thyroid hormone response elements and regulates transcription in response to thyroid hormones. |
format |
Article in Journal/Newspaper |
author |
Marchand, O Safi, R Escriva, H Van Rompaey, E Prunet, P Laudet, V |
author_facet |
Marchand, O Safi, R Escriva, H Van Rompaey, E Prunet, P Laudet, V |
author_sort |
Marchand, O |
title |
Molecular cloning and characterization of thyroid hormone receptors in teleost fish |
title_short |
Molecular cloning and characterization of thyroid hormone receptors in teleost fish |
title_full |
Molecular cloning and characterization of thyroid hormone receptors in teleost fish |
title_fullStr |
Molecular cloning and characterization of thyroid hormone receptors in teleost fish |
title_full_unstemmed |
Molecular cloning and characterization of thyroid hormone receptors in teleost fish |
title_sort |
molecular cloning and characterization of thyroid hormone receptors in teleost fish |
publisher |
Bioscientifica |
publishDate |
2001 |
url |
http://dx.doi.org/10.1677/jme.0.0260051 https://jme.bioscientifica.com/view/journals/jme/26/1/51.xml https://jme.bioscientifica.com/downloadpdf/journals/jme/26/1/51.xml |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Journal of Molecular Endocrinology volume 26, issue 1, page 51-65 ISSN 0952-5041 1479-6813 |
op_doi |
https://doi.org/10.1677/jme.0.0260051 |
container_title |
Journal of Molecular Endocrinology |
container_volume |
26 |
container_issue |
1 |
container_start_page |
51 |
op_container_end_page |
65 |
_version_ |
1797580076268650496 |