Transgenic rainbow trout expressed sGnRH-antisense RNA under the control of sGnRH promoter of Atlantic salmon
A recombinant vector containing antisense DNA complementary to Atlantic salmon (Salmo salar) sGnRH cDNA driven by specific promoter Pab derived from a corresponding sGnRH gene was introduced into rainbow trout (Oncorhynchus mykiss) eggs. This resulted in transgenic animals that had integrated one co...
Published in: | Journal of Molecular Endocrinology |
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Online Access: | http://dx.doi.org/10.1677/jme.0.0250337 https://jme.bioscientifica.com/view/journals/jme/25/3/337.xml https://jme.bioscientifica.com/downloadpdf/journals/jme/25/3/337.xml |
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crbioscientif:10.1677/jme.0.0250337 2024-09-15T17:56:09+00:00 Transgenic rainbow trout expressed sGnRH-antisense RNA under the control of sGnRH promoter of Atlantic salmon Uzbekova, S Chyb, J Ferriere, F Bailhache, T Prunet, P Alestrom, P Breton, B 2000 http://dx.doi.org/10.1677/jme.0.0250337 https://jme.bioscientifica.com/view/journals/jme/25/3/337.xml https://jme.bioscientifica.com/downloadpdf/journals/jme/25/3/337.xml unknown Bioscientifica Journal of Molecular Endocrinology volume 25, issue 3, page 337-350 ISSN 0952-5041 1479-6813 journal-article 2000 crbioscientif https://doi.org/10.1677/jme.0.0250337 2024-07-25T04:09:57Z A recombinant vector containing antisense DNA complementary to Atlantic salmon (Salmo salar) sGnRH cDNA driven by specific promoter Pab derived from a corresponding sGnRH gene was introduced into rainbow trout (Oncorhynchus mykiss) eggs. This resulted in transgenic animals that had integrated one copy of the transgene into their genome and transmitted it through the germline. Antisense-sGnRH mRNA (AS) was expressed mainly in the brain of transgenic AS(+) fish. Levels of sGnRH endogenous mRNA in the brain were lower in 11-month-old AS(+) fish compared with nontransgenic AS(-) individuals from the same F2 progeny. sGnRH levels significantly decreased in the pituitary of transgenic males and females around the maturation period and in the brain of AS(+) immature females compared with controls. No reliable statistical difference was found in the levels of FSH and LH between AS(+) and AS(-) groups either in immature or mature fish. The majority of transgenic fish reached maturity at the same time as did nontransgenic individuals, although the maturation of AS(+) animals seemed to be more asynchronous. For the first time, the influence of antisense messengers on endogenous mRNA in transgenic fish and the corresponding protein is described. Article in Journal/Newspaper Atlantic salmon Salmo salar Bioscientifica Journal of Molecular Endocrinology 25 3 337 350 |
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Bioscientifica |
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crbioscientif |
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unknown |
description |
A recombinant vector containing antisense DNA complementary to Atlantic salmon (Salmo salar) sGnRH cDNA driven by specific promoter Pab derived from a corresponding sGnRH gene was introduced into rainbow trout (Oncorhynchus mykiss) eggs. This resulted in transgenic animals that had integrated one copy of the transgene into their genome and transmitted it through the germline. Antisense-sGnRH mRNA (AS) was expressed mainly in the brain of transgenic AS(+) fish. Levels of sGnRH endogenous mRNA in the brain were lower in 11-month-old AS(+) fish compared with nontransgenic AS(-) individuals from the same F2 progeny. sGnRH levels significantly decreased in the pituitary of transgenic males and females around the maturation period and in the brain of AS(+) immature females compared with controls. No reliable statistical difference was found in the levels of FSH and LH between AS(+) and AS(-) groups either in immature or mature fish. The majority of transgenic fish reached maturity at the same time as did nontransgenic individuals, although the maturation of AS(+) animals seemed to be more asynchronous. For the first time, the influence of antisense messengers on endogenous mRNA in transgenic fish and the corresponding protein is described. |
format |
Article in Journal/Newspaper |
author |
Uzbekova, S Chyb, J Ferriere, F Bailhache, T Prunet, P Alestrom, P Breton, B |
spellingShingle |
Uzbekova, S Chyb, J Ferriere, F Bailhache, T Prunet, P Alestrom, P Breton, B Transgenic rainbow trout expressed sGnRH-antisense RNA under the control of sGnRH promoter of Atlantic salmon |
author_facet |
Uzbekova, S Chyb, J Ferriere, F Bailhache, T Prunet, P Alestrom, P Breton, B |
author_sort |
Uzbekova, S |
title |
Transgenic rainbow trout expressed sGnRH-antisense RNA under the control of sGnRH promoter of Atlantic salmon |
title_short |
Transgenic rainbow trout expressed sGnRH-antisense RNA under the control of sGnRH promoter of Atlantic salmon |
title_full |
Transgenic rainbow trout expressed sGnRH-antisense RNA under the control of sGnRH promoter of Atlantic salmon |
title_fullStr |
Transgenic rainbow trout expressed sGnRH-antisense RNA under the control of sGnRH promoter of Atlantic salmon |
title_full_unstemmed |
Transgenic rainbow trout expressed sGnRH-antisense RNA under the control of sGnRH promoter of Atlantic salmon |
title_sort |
transgenic rainbow trout expressed sgnrh-antisense rna under the control of sgnrh promoter of atlantic salmon |
publisher |
Bioscientifica |
publishDate |
2000 |
url |
http://dx.doi.org/10.1677/jme.0.0250337 https://jme.bioscientifica.com/view/journals/jme/25/3/337.xml https://jme.bioscientifica.com/downloadpdf/journals/jme/25/3/337.xml |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Journal of Molecular Endocrinology volume 25, issue 3, page 337-350 ISSN 0952-5041 1479-6813 |
op_doi |
https://doi.org/10.1677/jme.0.0250337 |
container_title |
Journal of Molecular Endocrinology |
container_volume |
25 |
container_issue |
3 |
container_start_page |
337 |
op_container_end_page |
350 |
_version_ |
1810432368159227904 |