Angiotensin II receptors in the gill of sea water- and freshwater-adapted eel
ABSTRACT Immunocytochemistry of paraffin-embedded and cryostat sections of eel ( Anguilla anguilla ) gill showed that angiotensin II receptors (Ang II-R) were present in chloride cells, uniformly distributed in the cytoplasm and on surface membranes. Computerised image analysis of these preparations...
Published in: | Journal of Molecular Endocrinology |
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Bioscientifica
1997
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crbioscientif:10.1677/jme.0.0180067 2024-09-09T19:00:43+00:00 Angiotensin II receptors in the gill of sea water- and freshwater-adapted eel Marsigliante, S Muscella, A Vinson, G P Storelli, C 1997 http://dx.doi.org/10.1677/jme.0.0180067 https://jme.bioscientifica.com/view/journals/jme/18/1/jme_18_1_008.xml https://jme.bioscientifica.com/downloadpdf/journals/jme/18/1/jme_18_1_008.xml unknown Bioscientifica Journal of Molecular Endocrinology volume 18, issue 1, page 67-76 ISSN 0952-5041 1479-6813 journal-article 1997 crbioscientif https://doi.org/10.1677/jme.0.0180067 2024-06-27T04:10:49Z ABSTRACT Immunocytochemistry of paraffin-embedded and cryostat sections of eel ( Anguilla anguilla ) gill showed that angiotensin II receptors (Ang II-R) were present in chloride cells, uniformly distributed in the cytoplasm and on surface membranes. Computerised image analysis of these preparations showed that gills from sea water (SW)-adapted animals had a significantly (3-fold) higher Ang II-R concentration compared with freshwater (FW)-adapted eel gills. Isoelectric focusing gel electrophoresis revealed two Ang II-R isoforms with pI 6·5 and 6·6 that were differentially modulated by environmental salinity: they were equally abundant in SW while in FW the pI 6·6/pI 6·5 ratio was 1·66. Using catalytic cytochemistry with image analysis, gill chloride cell membrane Na + /K + ATPase activity was shown to increase 4-fold in response to SW adaptation. Additionally, perfusion of gills for 30 min with 0·1, 10 or with 100 n M Ang II provoked a dose-dependent increment in Na + /K + ATPase activity in FW, and a biphasic response in SW gills in which activity was significantly increased at low Ang II concentrations but was reduced to basal values at 100 n M . The data suggest that adaptation to sea water significantly increases Ang II-R concentration in the chloride cell and, together with the effects of Ang II on Na +/ K + ATPase activity, suggest a role for this hormone in gill NaCl retention. The different responses of Na + /K + ATPase to Ang II stimulation in FW and SW may be attributed to the presence of two receptor subtypes that are differently modulated by salinity and that have opposing effects on Na + /K + ATPase. Article in Journal/Newspaper Anguilla anguilla Bioscientifica Journal of Molecular Endocrinology 18 1 67 76 |
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ABSTRACT Immunocytochemistry of paraffin-embedded and cryostat sections of eel ( Anguilla anguilla ) gill showed that angiotensin II receptors (Ang II-R) were present in chloride cells, uniformly distributed in the cytoplasm and on surface membranes. Computerised image analysis of these preparations showed that gills from sea water (SW)-adapted animals had a significantly (3-fold) higher Ang II-R concentration compared with freshwater (FW)-adapted eel gills. Isoelectric focusing gel electrophoresis revealed two Ang II-R isoforms with pI 6·5 and 6·6 that were differentially modulated by environmental salinity: they were equally abundant in SW while in FW the pI 6·6/pI 6·5 ratio was 1·66. Using catalytic cytochemistry with image analysis, gill chloride cell membrane Na + /K + ATPase activity was shown to increase 4-fold in response to SW adaptation. Additionally, perfusion of gills for 30 min with 0·1, 10 or with 100 n M Ang II provoked a dose-dependent increment in Na + /K + ATPase activity in FW, and a biphasic response in SW gills in which activity was significantly increased at low Ang II concentrations but was reduced to basal values at 100 n M . The data suggest that adaptation to sea water significantly increases Ang II-R concentration in the chloride cell and, together with the effects of Ang II on Na +/ K + ATPase activity, suggest a role for this hormone in gill NaCl retention. The different responses of Na + /K + ATPase to Ang II stimulation in FW and SW may be attributed to the presence of two receptor subtypes that are differently modulated by salinity and that have opposing effects on Na + /K + ATPase. |
format |
Article in Journal/Newspaper |
author |
Marsigliante, S Muscella, A Vinson, G P Storelli, C |
spellingShingle |
Marsigliante, S Muscella, A Vinson, G P Storelli, C Angiotensin II receptors in the gill of sea water- and freshwater-adapted eel |
author_facet |
Marsigliante, S Muscella, A Vinson, G P Storelli, C |
author_sort |
Marsigliante, S |
title |
Angiotensin II receptors in the gill of sea water- and freshwater-adapted eel |
title_short |
Angiotensin II receptors in the gill of sea water- and freshwater-adapted eel |
title_full |
Angiotensin II receptors in the gill of sea water- and freshwater-adapted eel |
title_fullStr |
Angiotensin II receptors in the gill of sea water- and freshwater-adapted eel |
title_full_unstemmed |
Angiotensin II receptors in the gill of sea water- and freshwater-adapted eel |
title_sort |
angiotensin ii receptors in the gill of sea water- and freshwater-adapted eel |
publisher |
Bioscientifica |
publishDate |
1997 |
url |
http://dx.doi.org/10.1677/jme.0.0180067 https://jme.bioscientifica.com/view/journals/jme/18/1/jme_18_1_008.xml https://jme.bioscientifica.com/downloadpdf/journals/jme/18/1/jme_18_1_008.xml |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
Journal of Molecular Endocrinology volume 18, issue 1, page 67-76 ISSN 0952-5041 1479-6813 |
op_doi |
https://doi.org/10.1677/jme.0.0180067 |
container_title |
Journal of Molecular Endocrinology |
container_volume |
18 |
container_issue |
1 |
container_start_page |
67 |
op_container_end_page |
76 |
_version_ |
1809941661378150400 |