Sex steroids differentially regulate fshb, lhb and gnrhr expression in Atlantic cod (Gadus morhua)
Depending on the stage of gonad maturation, as well as other factors, gonadal steroids can exert either a positive or negative feedback at the brain and pituitary level. While this has been demonstrated in many teleost species, little is known about the nature of steroid feedback in Gadiform fish. U...
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crbioscientif:10.1530/rep-17-0208 2024-09-30T14:32:10+00:00 Sex steroids differentially regulate fshb, lhb and gnrhr expression in Atlantic cod (Gadus morhua) von Krogh, Kristine Bjørndal, Gunnveig Toft Nourizadeh-Lillabadi, Rasoul Hodne, Kjetil Ropstad, Erik Haug, Trude M Weltzien, Finn-Arne 2017 http://dx.doi.org/10.1530/rep-17-0208 https://rep.bioscientifica.com/view/journals/rep/154/5/REP-17-0208.xml https://syndication.highwire.org/content/doi/10.1530/REP-17-0208 unknown Bioscientifica Reproduction volume 154, issue 5, page 581-594 ISSN 1470-1626 1741-7899 journal-article 2017 crbioscientif https://doi.org/10.1530/rep-17-0208 2024-09-19T04:09:39Z Depending on the stage of gonad maturation, as well as other factors, gonadal steroids can exert either a positive or negative feedback at the brain and pituitary level. While this has been demonstrated in many teleost species, little is known about the nature of steroid feedback in Gadiform fish. Using an optimized in vitro model system of the Atlantic cod pituitary, the present study investigated the potential effects of two physiologically relevant doses of estradiol, testosterone (TS) or dihydrotestosterone (DHTS) on cell viability and gene expression of gonadotropin subunits ( fshb / lhb ) and two suggested reproduction-relevant gonadotropin-releasing hormone receptors ( gnrhr1b / gnrhr2a ) during three stages of sexual maturity. In general, all steroids stimulated cell viability in terms of metabolic activity and membrane integrity. Furthermore, all steroids affected fshb expression, with the effect depending on both the specific steroid, dose and maturity status. Conversely, only DHTS exposure affected lhb levels, and this occurred only during the spawning season. Using single-cell qPCR, co-transcription of gnrhr1b and gnrhr2a was confirmed to both fshb- and lhb- expressing gonadotropes, with gnrhr2a being the most prominently expressed isoform. While steroid exposure had no effect on gnrhr1b expression, all steroids affected gnrhr2a transcript levels in at least one maturity stage. These and previous results from our group point to Gnrhr2a as the main modulator of gonadotropin regulation in cod and that regulation of its gene expression level might function as a direct mechanism for steroid feedback at the pituitary level. Article in Journal/Newspaper atlantic cod Gadus morhua Bioscientifica Reproduction 154 5 581 594 |
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Bioscientifica |
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Depending on the stage of gonad maturation, as well as other factors, gonadal steroids can exert either a positive or negative feedback at the brain and pituitary level. While this has been demonstrated in many teleost species, little is known about the nature of steroid feedback in Gadiform fish. Using an optimized in vitro model system of the Atlantic cod pituitary, the present study investigated the potential effects of two physiologically relevant doses of estradiol, testosterone (TS) or dihydrotestosterone (DHTS) on cell viability and gene expression of gonadotropin subunits ( fshb / lhb ) and two suggested reproduction-relevant gonadotropin-releasing hormone receptors ( gnrhr1b / gnrhr2a ) during three stages of sexual maturity. In general, all steroids stimulated cell viability in terms of metabolic activity and membrane integrity. Furthermore, all steroids affected fshb expression, with the effect depending on both the specific steroid, dose and maturity status. Conversely, only DHTS exposure affected lhb levels, and this occurred only during the spawning season. Using single-cell qPCR, co-transcription of gnrhr1b and gnrhr2a was confirmed to both fshb- and lhb- expressing gonadotropes, with gnrhr2a being the most prominently expressed isoform. While steroid exposure had no effect on gnrhr1b expression, all steroids affected gnrhr2a transcript levels in at least one maturity stage. These and previous results from our group point to Gnrhr2a as the main modulator of gonadotropin regulation in cod and that regulation of its gene expression level might function as a direct mechanism for steroid feedback at the pituitary level. |
format |
Article in Journal/Newspaper |
author |
von Krogh, Kristine Bjørndal, Gunnveig Toft Nourizadeh-Lillabadi, Rasoul Hodne, Kjetil Ropstad, Erik Haug, Trude M Weltzien, Finn-Arne |
spellingShingle |
von Krogh, Kristine Bjørndal, Gunnveig Toft Nourizadeh-Lillabadi, Rasoul Hodne, Kjetil Ropstad, Erik Haug, Trude M Weltzien, Finn-Arne Sex steroids differentially regulate fshb, lhb and gnrhr expression in Atlantic cod (Gadus morhua) |
author_facet |
von Krogh, Kristine Bjørndal, Gunnveig Toft Nourizadeh-Lillabadi, Rasoul Hodne, Kjetil Ropstad, Erik Haug, Trude M Weltzien, Finn-Arne |
author_sort |
von Krogh, Kristine |
title |
Sex steroids differentially regulate fshb, lhb and gnrhr expression in Atlantic cod (Gadus morhua) |
title_short |
Sex steroids differentially regulate fshb, lhb and gnrhr expression in Atlantic cod (Gadus morhua) |
title_full |
Sex steroids differentially regulate fshb, lhb and gnrhr expression in Atlantic cod (Gadus morhua) |
title_fullStr |
Sex steroids differentially regulate fshb, lhb and gnrhr expression in Atlantic cod (Gadus morhua) |
title_full_unstemmed |
Sex steroids differentially regulate fshb, lhb and gnrhr expression in Atlantic cod (Gadus morhua) |
title_sort |
sex steroids differentially regulate fshb, lhb and gnrhr expression in atlantic cod (gadus morhua) |
publisher |
Bioscientifica |
publishDate |
2017 |
url |
http://dx.doi.org/10.1530/rep-17-0208 https://rep.bioscientifica.com/view/journals/rep/154/5/REP-17-0208.xml https://syndication.highwire.org/content/doi/10.1530/REP-17-0208 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
Reproduction volume 154, issue 5, page 581-594 ISSN 1470-1626 1741-7899 |
op_doi |
https://doi.org/10.1530/rep-17-0208 |
container_title |
Reproduction |
container_volume |
154 |
container_issue |
5 |
container_start_page |
581 |
op_container_end_page |
594 |
_version_ |
1811636397244678144 |