Relation between serum xenobiotic-induced receptor activities and sperm DNA damage and sperm apoptotic markers in European and Inuit populations
Persistent organic pollutants (POPs) can interfere with hormone activities and are suspected as endocrine disrupters involved in disorders, e.g. reproductive disorders. We investigated the possible relation between the actual integrated serum xenoestrogenic, xenoandrogenic and aryl hydrocarbon recep...
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crbioscientif:10.1530/rep-06-0195 2023-12-31T10:07:30+01:00 Relation between serum xenobiotic-induced receptor activities and sperm DNA damage and sperm apoptotic markers in European and Inuit populations Long, Manhai Stronati, Alessandra Bizzaro, Davide Krüger, Tanja Manicardi, Gian-Carlo Hjelmborg, Philip S Spanò, Marcello Giwercman, Alexander Toft, Gunnar Bonde, Jens Peter Bonefeld-Jorgensen, Eva C 2007 http://dx.doi.org/10.1530/rep-06-0195 https://rep.bioscientifica.com/view/journals/rep/133/2/1330517.xml https://rep.bioscientifica.com/downloadpdf/journals/rep/133/2/1330517.xml unknown Bioscientifica Reproduction volume 133, issue 2, page 517-530 ISSN 1470-1626 1741-7899 Cell Biology Obstetrics and Gynecology Endocrinology Embryology Reproductive Medicine journal-article 2007 crbioscientif https://doi.org/10.1530/rep-06-0195 2023-12-07T16:15:39Z Persistent organic pollutants (POPs) can interfere with hormone activities and are suspected as endocrine disrupters involved in disorders, e.g. reproductive disorders. We investigated the possible relation between the actual integrated serum xenoestrogenic, xenoandrogenic and aryl hydrocarbon receptor (AhR) activities, and the sperm DNA damage and sperm apoptotic markers of 262 adult males (54 Inuits from Greenland, 69 from Warsaw (Poland), 81 from Sweden, and 58 from Kharkiv (Ukraine)) exposed to different levels of POPs. Xenobiotic-induced receptor activities were determined by receptor-mediated luciferase reporter gene expression. Sperm DNA damage was measured using terminal deoxynucleotidyl transferase-driven dUTP nick labeling assay (TUNEL) and pro- (Fas) and anti-apoptotic (Bcl-xL) markers were determined by immune methods. Different features of xenobiotic-induced receptor activity in serum and sperm DNA fragmentation and apoptotic markers existed between the Inuits and the European Caucasians. Negative correlations between xenobiotic-induced receptor activities and DNA damage were found for Inuits having relatively lower xenoestrogenic, lower dioxin-like activity, and lower sperm DNA damage, but higher xenoandrogenic activity. In contrast, in the European groups, xenobiotic-induced receptor activities were found to be positively correlated with the DNA damage. Further research must elucidate whether altered receptor activities in concerted action with genetic and/or nutrient factors may have protecting effect on sperm DNA damage of the Inuit population. Article in Journal/Newspaper Greenland inuit inuits Bioscientifica (via Crossref) Reproduction 133 2 517 530 |
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Open Polar |
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Bioscientifica (via Crossref) |
op_collection_id |
crbioscientif |
language |
unknown |
topic |
Cell Biology Obstetrics and Gynecology Endocrinology Embryology Reproductive Medicine |
spellingShingle |
Cell Biology Obstetrics and Gynecology Endocrinology Embryology Reproductive Medicine Long, Manhai Stronati, Alessandra Bizzaro, Davide Krüger, Tanja Manicardi, Gian-Carlo Hjelmborg, Philip S Spanò, Marcello Giwercman, Alexander Toft, Gunnar Bonde, Jens Peter Bonefeld-Jorgensen, Eva C Relation between serum xenobiotic-induced receptor activities and sperm DNA damage and sperm apoptotic markers in European and Inuit populations |
topic_facet |
Cell Biology Obstetrics and Gynecology Endocrinology Embryology Reproductive Medicine |
description |
Persistent organic pollutants (POPs) can interfere with hormone activities and are suspected as endocrine disrupters involved in disorders, e.g. reproductive disorders. We investigated the possible relation between the actual integrated serum xenoestrogenic, xenoandrogenic and aryl hydrocarbon receptor (AhR) activities, and the sperm DNA damage and sperm apoptotic markers of 262 adult males (54 Inuits from Greenland, 69 from Warsaw (Poland), 81 from Sweden, and 58 from Kharkiv (Ukraine)) exposed to different levels of POPs. Xenobiotic-induced receptor activities were determined by receptor-mediated luciferase reporter gene expression. Sperm DNA damage was measured using terminal deoxynucleotidyl transferase-driven dUTP nick labeling assay (TUNEL) and pro- (Fas) and anti-apoptotic (Bcl-xL) markers were determined by immune methods. Different features of xenobiotic-induced receptor activity in serum and sperm DNA fragmentation and apoptotic markers existed between the Inuits and the European Caucasians. Negative correlations between xenobiotic-induced receptor activities and DNA damage were found for Inuits having relatively lower xenoestrogenic, lower dioxin-like activity, and lower sperm DNA damage, but higher xenoandrogenic activity. In contrast, in the European groups, xenobiotic-induced receptor activities were found to be positively correlated with the DNA damage. Further research must elucidate whether altered receptor activities in concerted action with genetic and/or nutrient factors may have protecting effect on sperm DNA damage of the Inuit population. |
format |
Article in Journal/Newspaper |
author |
Long, Manhai Stronati, Alessandra Bizzaro, Davide Krüger, Tanja Manicardi, Gian-Carlo Hjelmborg, Philip S Spanò, Marcello Giwercman, Alexander Toft, Gunnar Bonde, Jens Peter Bonefeld-Jorgensen, Eva C |
author_facet |
Long, Manhai Stronati, Alessandra Bizzaro, Davide Krüger, Tanja Manicardi, Gian-Carlo Hjelmborg, Philip S Spanò, Marcello Giwercman, Alexander Toft, Gunnar Bonde, Jens Peter Bonefeld-Jorgensen, Eva C |
author_sort |
Long, Manhai |
title |
Relation between serum xenobiotic-induced receptor activities and sperm DNA damage and sperm apoptotic markers in European and Inuit populations |
title_short |
Relation between serum xenobiotic-induced receptor activities and sperm DNA damage and sperm apoptotic markers in European and Inuit populations |
title_full |
Relation between serum xenobiotic-induced receptor activities and sperm DNA damage and sperm apoptotic markers in European and Inuit populations |
title_fullStr |
Relation between serum xenobiotic-induced receptor activities and sperm DNA damage and sperm apoptotic markers in European and Inuit populations |
title_full_unstemmed |
Relation between serum xenobiotic-induced receptor activities and sperm DNA damage and sperm apoptotic markers in European and Inuit populations |
title_sort |
relation between serum xenobiotic-induced receptor activities and sperm dna damage and sperm apoptotic markers in european and inuit populations |
publisher |
Bioscientifica |
publishDate |
2007 |
url |
http://dx.doi.org/10.1530/rep-06-0195 https://rep.bioscientifica.com/view/journals/rep/133/2/1330517.xml https://rep.bioscientifica.com/downloadpdf/journals/rep/133/2/1330517.xml |
genre |
Greenland inuit inuits |
genre_facet |
Greenland inuit inuits |
op_source |
Reproduction volume 133, issue 2, page 517-530 ISSN 1470-1626 1741-7899 |
op_doi |
https://doi.org/10.1530/rep-06-0195 |
container_title |
Reproduction |
container_volume |
133 |
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2 |
container_start_page |
517 |
op_container_end_page |
530 |
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1786839918565130240 |