Sexually dimorphic transcription of estrogen receptors in cod gonads throughout a reproductive cycle
The role of sex steroid regulation in gonadal maturation is a very complex process that is far from being fully understood. Hence, we have investigated seasonal changes in gonadal expression of estrogen receptors (ERs) in Atlantic cod ( Gadus morhua L.), a batch spawner, throughout the annual reprod...
Published in: | Journal of Molecular Endocrinology |
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crbioscientif:10.1530/jme-13-0187 2024-06-23T07:51:04+00:00 Sexually dimorphic transcription of estrogen receptors in cod gonads throughout a reproductive cycle Nagasawa, Kazue Presslauer, Christopher Kirtiklis, Lech Babiak, Igor Fernandes, Jorge M O 2014 http://dx.doi.org/10.1530/jme-13-0187 https://jme.bioscientifica.com/view/journals/jme/52/3/357.xml https://jme.bioscientifica.com/downloadpdf/journals/jme/52/3/357.xml unknown Bioscientifica Journal of Molecular Endocrinology volume 52, issue 3, page 357-371 ISSN 0952-5041 1479-6813 journal-article 2014 crbioscientif https://doi.org/10.1530/jme-13-0187 2024-05-30T08:13:38Z The role of sex steroid regulation in gonadal maturation is a very complex process that is far from being fully understood. Hence, we have investigated seasonal changes in gonadal expression of estrogen receptors (ERs) in Atlantic cod ( Gadus morhua L.), a batch spawner, throughout the annual reproductive cycle. Three nuclear ER partial cDNA sequences ( esr1 , esr2a , and esr2b ) were cloned and all esr transcripts were detected mainly in liver and gonads of fish of both sexes. In situ hybridization of esr s along with germ cell ( vasa ) and gonadal somatic cell markers (gonadal soma-derived factor ( gsdf ), 3β-hydroxysteroid dehydrogenase ( 3 β hsd ), and anti-Müllerian hormone ( amh ) for testicular, or gsdf for ovarian somatic cells) showed that all three esr s were preferentially localized within interstitial fibroblasts composed of immature and mature Leydig cells in testis, whereas they were differentially expressed in both follicular cells and oocytes in ovary. Quantitative real-time PCR analysis revealed a sexually dimorphic expression pattern of the three esr paralogs in testis and ovary. A significant increase in esr2a expression was identified in testis and of esr2b in ovary, whereas e sr1 transcripts were elevated in both testis and ovary in February and March before the spawning period. The localization and sexually dimorphic expression of esr genes in gonads indicate a direct function of estrogen via ERs in gonadal somatic cell growth and differentiation for Leydig cell in testis and follicular cells in ovary throughout the annual reproductive cycle in Atlantic cod. Article in Journal/Newspaper atlantic cod Gadus morhua Bioscientifica Vasa ENVELOPE(25.177,25.177,67.587,67.587) Journal of Molecular Endocrinology 52 3 357 371 |
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Bioscientifica |
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crbioscientif |
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The role of sex steroid regulation in gonadal maturation is a very complex process that is far from being fully understood. Hence, we have investigated seasonal changes in gonadal expression of estrogen receptors (ERs) in Atlantic cod ( Gadus morhua L.), a batch spawner, throughout the annual reproductive cycle. Three nuclear ER partial cDNA sequences ( esr1 , esr2a , and esr2b ) were cloned and all esr transcripts were detected mainly in liver and gonads of fish of both sexes. In situ hybridization of esr s along with germ cell ( vasa ) and gonadal somatic cell markers (gonadal soma-derived factor ( gsdf ), 3β-hydroxysteroid dehydrogenase ( 3 β hsd ), and anti-Müllerian hormone ( amh ) for testicular, or gsdf for ovarian somatic cells) showed that all three esr s were preferentially localized within interstitial fibroblasts composed of immature and mature Leydig cells in testis, whereas they were differentially expressed in both follicular cells and oocytes in ovary. Quantitative real-time PCR analysis revealed a sexually dimorphic expression pattern of the three esr paralogs in testis and ovary. A significant increase in esr2a expression was identified in testis and of esr2b in ovary, whereas e sr1 transcripts were elevated in both testis and ovary in February and March before the spawning period. The localization and sexually dimorphic expression of esr genes in gonads indicate a direct function of estrogen via ERs in gonadal somatic cell growth and differentiation for Leydig cell in testis and follicular cells in ovary throughout the annual reproductive cycle in Atlantic cod. |
format |
Article in Journal/Newspaper |
author |
Nagasawa, Kazue Presslauer, Christopher Kirtiklis, Lech Babiak, Igor Fernandes, Jorge M O |
spellingShingle |
Nagasawa, Kazue Presslauer, Christopher Kirtiklis, Lech Babiak, Igor Fernandes, Jorge M O Sexually dimorphic transcription of estrogen receptors in cod gonads throughout a reproductive cycle |
author_facet |
Nagasawa, Kazue Presslauer, Christopher Kirtiklis, Lech Babiak, Igor Fernandes, Jorge M O |
author_sort |
Nagasawa, Kazue |
title |
Sexually dimorphic transcription of estrogen receptors in cod gonads throughout a reproductive cycle |
title_short |
Sexually dimorphic transcription of estrogen receptors in cod gonads throughout a reproductive cycle |
title_full |
Sexually dimorphic transcription of estrogen receptors in cod gonads throughout a reproductive cycle |
title_fullStr |
Sexually dimorphic transcription of estrogen receptors in cod gonads throughout a reproductive cycle |
title_full_unstemmed |
Sexually dimorphic transcription of estrogen receptors in cod gonads throughout a reproductive cycle |
title_sort |
sexually dimorphic transcription of estrogen receptors in cod gonads throughout a reproductive cycle |
publisher |
Bioscientifica |
publishDate |
2014 |
url |
http://dx.doi.org/10.1530/jme-13-0187 https://jme.bioscientifica.com/view/journals/jme/52/3/357.xml https://jme.bioscientifica.com/downloadpdf/journals/jme/52/3/357.xml |
long_lat |
ENVELOPE(25.177,25.177,67.587,67.587) |
geographic |
Vasa |
geographic_facet |
Vasa |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
Journal of Molecular Endocrinology volume 52, issue 3, page 357-371 ISSN 0952-5041 1479-6813 |
op_doi |
https://doi.org/10.1530/jme-13-0187 |
container_title |
Journal of Molecular Endocrinology |
container_volume |
52 |
container_issue |
3 |
container_start_page |
357 |
op_container_end_page |
371 |
_version_ |
1802642061459980288 |