Influence of Betaine- and Choline-based Eutectic Solvents on Lipase Activity
Background: Eutectic solvents are a mixture of two compounds which possess a lower melting temperature than the parent compounds, using quaternary ammonium salts, such as choline chloride and betaine hydrochloride and organic acids, polyols and amides as hydrogen bond donors. These solvents can be a...
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crbenthamsciepub:10.2174/2212711906666190710181629 2024-09-30T14:23:10+00:00 Influence of Betaine- and Choline-based Eutectic Solvents on Lipase Activity Ribeiro, Bernardo Dias de Carvalho Iff, Lucas Coelho, Maria Alice Zarur Marrucho, Isabel M. Conselho Nacional de Desenvolvimento Científico e Tecnológico, CNPq Fundação para a Ciência e Tecnologia/MCTES, Portugal 2019 http://dx.doi.org/10.2174/2212711906666190710181629 http://eurekaselect.com/article/download/173400 en eng Bentham Science Publishers Ltd. Current Biochemical Engineering volume 5, issue 1, page 57-68 ISSN 2212-7119 journal-article 2019 crbenthamsciepub https://doi.org/10.2174/2212711906666190710181629 2024-09-05T04:10:49Z Background: Eutectic solvents are a mixture of two compounds which possess a lower melting temperature than the parent compounds, using quaternary ammonium salts, such as choline chloride and betaine hydrochloride and organic acids, polyols and amides as hydrogen bond donors. These solvents can be an alternative as non-aqueous media for enzymatic reactions, mainly using lipases. Objective: The objective of this work is to evaluate enzymatic activity and stability of commercial lipases, immobilized or at free form (Thermomyces lanuginosus: Lipozyme TL IM, iTL and Lipolase 100 L, fTL; Candida antarctica: Novozym 435, iCALB; Novozym 735, iCALA and Novozym CALB L, fCALB); and a phospholipase (Lecitase Ultra), in the presence of eutectic solvents (choline chloride ChCl:urea, ChCl:glycerol, betaine hydrochloride (BeHCl):urea and BeHCl: glycerol. Methods: Initially, lipases were maintained for 2 hours in solutions of choline and betaine-based eutectic solvents (1 to 20% m/m) at 25ºC compared with water for relative enzymatic activity. Using the solvent that best promoted lipase activity, some parameters were evaluated such as the molar ratio between quaternary ammonium salts and urea, stocking temperature and kinetics. Results and Conclusion: These eutectic solvents enable, mainly with immobilized lipases, 25 to 125 times more activity than water at 25ºC and 2h, and even after 24h, lipase iTLL was still 40 times more active in the presence of ChCl:Urea 1:3. Lipase iCALB showed great thermostability 47 times higher at 55ºC, almost double relative activity at 25ºC in the presence of BetHCl:Urea 1:4. Article in Journal/Newspaper Antarc* Antarctica Bentham Science Publishers Current Biochemical Engineering 5 1 57 68 |
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Bentham Science Publishers |
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English |
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Background: Eutectic solvents are a mixture of two compounds which possess a lower melting temperature than the parent compounds, using quaternary ammonium salts, such as choline chloride and betaine hydrochloride and organic acids, polyols and amides as hydrogen bond donors. These solvents can be an alternative as non-aqueous media for enzymatic reactions, mainly using lipases. Objective: The objective of this work is to evaluate enzymatic activity and stability of commercial lipases, immobilized or at free form (Thermomyces lanuginosus: Lipozyme TL IM, iTL and Lipolase 100 L, fTL; Candida antarctica: Novozym 435, iCALB; Novozym 735, iCALA and Novozym CALB L, fCALB); and a phospholipase (Lecitase Ultra), in the presence of eutectic solvents (choline chloride ChCl:urea, ChCl:glycerol, betaine hydrochloride (BeHCl):urea and BeHCl: glycerol. Methods: Initially, lipases were maintained for 2 hours in solutions of choline and betaine-based eutectic solvents (1 to 20% m/m) at 25ºC compared with water for relative enzymatic activity. Using the solvent that best promoted lipase activity, some parameters were evaluated such as the molar ratio between quaternary ammonium salts and urea, stocking temperature and kinetics. Results and Conclusion: These eutectic solvents enable, mainly with immobilized lipases, 25 to 125 times more activity than water at 25ºC and 2h, and even after 24h, lipase iTLL was still 40 times more active in the presence of ChCl:Urea 1:3. Lipase iCALB showed great thermostability 47 times higher at 55ºC, almost double relative activity at 25ºC in the presence of BetHCl:Urea 1:4. |
author2 |
Conselho Nacional de Desenvolvimento Científico e Tecnológico, CNPq Fundação para a Ciência e Tecnologia/MCTES, Portugal |
format |
Article in Journal/Newspaper |
author |
Ribeiro, Bernardo Dias de Carvalho Iff, Lucas Coelho, Maria Alice Zarur Marrucho, Isabel M. |
spellingShingle |
Ribeiro, Bernardo Dias de Carvalho Iff, Lucas Coelho, Maria Alice Zarur Marrucho, Isabel M. Influence of Betaine- and Choline-based Eutectic Solvents on Lipase Activity |
author_facet |
Ribeiro, Bernardo Dias de Carvalho Iff, Lucas Coelho, Maria Alice Zarur Marrucho, Isabel M. |
author_sort |
Ribeiro, Bernardo Dias |
title |
Influence of Betaine- and Choline-based Eutectic Solvents on Lipase Activity |
title_short |
Influence of Betaine- and Choline-based Eutectic Solvents on Lipase Activity |
title_full |
Influence of Betaine- and Choline-based Eutectic Solvents on Lipase Activity |
title_fullStr |
Influence of Betaine- and Choline-based Eutectic Solvents on Lipase Activity |
title_full_unstemmed |
Influence of Betaine- and Choline-based Eutectic Solvents on Lipase Activity |
title_sort |
influence of betaine- and choline-based eutectic solvents on lipase activity |
publisher |
Bentham Science Publishers Ltd. |
publishDate |
2019 |
url |
http://dx.doi.org/10.2174/2212711906666190710181629 http://eurekaselect.com/article/download/173400 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Current Biochemical Engineering volume 5, issue 1, page 57-68 ISSN 2212-7119 |
op_doi |
https://doi.org/10.2174/2212711906666190710181629 |
container_title |
Current Biochemical Engineering |
container_volume |
5 |
container_issue |
1 |
container_start_page |
57 |
op_container_end_page |
68 |
_version_ |
1811636624226779136 |