New Microsatellite Markers for the Common Tern (Sterna hirundo) Developed with 454 Shot-Gun Pyrosequencing
Long term studies, focusing on population- and socio-biology research, require the unequivocal identification of individuals. DNA studies with Short Tandem Repeats (STR loci) became a widespread tool in population genetics. We used the next-generation sequencing (NGS) approach with 454 shot-gun pyro...
Published in: | The Open Ornithology Journal |
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crbenthamsciepub:10.2174/1874453201609010050 2024-09-15T18:00:00+00:00 New Microsatellite Markers for the Common Tern (Sterna hirundo) Developed with 454 Shot-Gun Pyrosequencing Janowski, Susann Gross, Ina Sauer-Gürth, Hedwig Tietze, Dieter Thomas Grohme, Markus Frohme, Marcus Becker, Peter Wink, Michael 2016 http://dx.doi.org/10.2174/1874453201609010050 https://openornithologyjournal.com/contents/volumes/V9/TOOENIJ-9-50/TOOENIJ-9-50.pdf https://openornithologyjournal.com/contents/volumes/V9/TOOENIJ-9-50/TOOENIJ-9-50.xml en eng Bentham Science Publishers Ltd. https://creativecommons.org/licenses/by-nc/4.0/legalcode The Open Ornithology Journal volume 9, issue 1, page 50-59 ISSN 1874-4532 journal-article 2016 crbenthamsciepub https://doi.org/10.2174/1874453201609010050 2024-06-27T04:10:44Z Long term studies, focusing on population- and socio-biology research, require the unequivocal identification of individuals. DNA studies with Short Tandem Repeats (STR loci) became a widespread tool in population genetics. We used the next-generation sequencing (NGS) approach with 454 shot-gun pyrosequencing to identify 13 new polymorphic STR loci for the Common Tern, Sterna hirundo . To enlarge the marker set we added two more loci originally developed for Black-legged Kittiwake ( Rissa tridactyla ) and Red-billed Gull ( Chroicocephalus scopulinus ) and arranged these 15 loci into three multiplex PCR panels for high throughput genotyping. Loci characterization demonstrated that our marker set is of high quality. A PIC value of about 0.67 and a power of exclusion value of 0.99 were reached. Deviation from Hardy-Weinberg expectations of some loci and low frequencies for null alleles are interpreted as a result of inbreeding and founder effect in the investigated tern colony. We used a test data set of this well-studied breeding colony of Common Tern at Banter Lake, Wilhelmshaven, Germany, to perform a parentage test. Parent-chick relationships, known from the social pedigree of that colony, were compared with genetically calculated ones. In order to test our markers and the used parentage program COLONY, we conducted six competing data sets with varying completeness of included parental genotypes. By including fully sampled parent pairs of known family assignment, results were correct for nest mates, single parents and parent pairs. Our marker set provides a powerful tool to investigate life-time reproductive success and other issues of population and socio-biology for Common Terns, e.g . in the aforementioned colony monitored for decades. Article in Journal/Newspaper Black-legged Kittiwake Common tern rissa tridactyla Sterna hirundo Bentham Science Publishers The Open Ornithology Journal 9 1 50 59 |
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Open Polar |
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Bentham Science Publishers |
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crbenthamsciepub |
language |
English |
description |
Long term studies, focusing on population- and socio-biology research, require the unequivocal identification of individuals. DNA studies with Short Tandem Repeats (STR loci) became a widespread tool in population genetics. We used the next-generation sequencing (NGS) approach with 454 shot-gun pyrosequencing to identify 13 new polymorphic STR loci for the Common Tern, Sterna hirundo . To enlarge the marker set we added two more loci originally developed for Black-legged Kittiwake ( Rissa tridactyla ) and Red-billed Gull ( Chroicocephalus scopulinus ) and arranged these 15 loci into three multiplex PCR panels for high throughput genotyping. Loci characterization demonstrated that our marker set is of high quality. A PIC value of about 0.67 and a power of exclusion value of 0.99 were reached. Deviation from Hardy-Weinberg expectations of some loci and low frequencies for null alleles are interpreted as a result of inbreeding and founder effect in the investigated tern colony. We used a test data set of this well-studied breeding colony of Common Tern at Banter Lake, Wilhelmshaven, Germany, to perform a parentage test. Parent-chick relationships, known from the social pedigree of that colony, were compared with genetically calculated ones. In order to test our markers and the used parentage program COLONY, we conducted six competing data sets with varying completeness of included parental genotypes. By including fully sampled parent pairs of known family assignment, results were correct for nest mates, single parents and parent pairs. Our marker set provides a powerful tool to investigate life-time reproductive success and other issues of population and socio-biology for Common Terns, e.g . in the aforementioned colony monitored for decades. |
format |
Article in Journal/Newspaper |
author |
Janowski, Susann Gross, Ina Sauer-Gürth, Hedwig Tietze, Dieter Thomas Grohme, Markus Frohme, Marcus Becker, Peter Wink, Michael |
spellingShingle |
Janowski, Susann Gross, Ina Sauer-Gürth, Hedwig Tietze, Dieter Thomas Grohme, Markus Frohme, Marcus Becker, Peter Wink, Michael New Microsatellite Markers for the Common Tern (Sterna hirundo) Developed with 454 Shot-Gun Pyrosequencing |
author_facet |
Janowski, Susann Gross, Ina Sauer-Gürth, Hedwig Tietze, Dieter Thomas Grohme, Markus Frohme, Marcus Becker, Peter Wink, Michael |
author_sort |
Janowski, Susann |
title |
New Microsatellite Markers for the Common Tern (Sterna hirundo) Developed with 454 Shot-Gun Pyrosequencing |
title_short |
New Microsatellite Markers for the Common Tern (Sterna hirundo) Developed with 454 Shot-Gun Pyrosequencing |
title_full |
New Microsatellite Markers for the Common Tern (Sterna hirundo) Developed with 454 Shot-Gun Pyrosequencing |
title_fullStr |
New Microsatellite Markers for the Common Tern (Sterna hirundo) Developed with 454 Shot-Gun Pyrosequencing |
title_full_unstemmed |
New Microsatellite Markers for the Common Tern (Sterna hirundo) Developed with 454 Shot-Gun Pyrosequencing |
title_sort |
new microsatellite markers for the common tern (sterna hirundo) developed with 454 shot-gun pyrosequencing |
publisher |
Bentham Science Publishers Ltd. |
publishDate |
2016 |
url |
http://dx.doi.org/10.2174/1874453201609010050 https://openornithologyjournal.com/contents/volumes/V9/TOOENIJ-9-50/TOOENIJ-9-50.pdf https://openornithologyjournal.com/contents/volumes/V9/TOOENIJ-9-50/TOOENIJ-9-50.xml |
genre |
Black-legged Kittiwake Common tern rissa tridactyla Sterna hirundo |
genre_facet |
Black-legged Kittiwake Common tern rissa tridactyla Sterna hirundo |
op_source |
The Open Ornithology Journal volume 9, issue 1, page 50-59 ISSN 1874-4532 |
op_rights |
https://creativecommons.org/licenses/by-nc/4.0/legalcode |
op_doi |
https://doi.org/10.2174/1874453201609010050 |
container_title |
The Open Ornithology Journal |
container_volume |
9 |
container_issue |
1 |
container_start_page |
50 |
op_container_end_page |
59 |
_version_ |
1810437115077459968 |