Cryomicroscopic Investigations of Freezing Processes in Cell Suspensions
This study evaluates the freezing response of three different cell types, Pacific oyster embryos (~50 µm in diameter), Jurkat cells and HeLa cells (~12 to 15 µm’s in diameter), using cryomicroscopy. Freezing experiments were performed on oyster embryos at cooling rates of either 5 or 10 °C/min, whil...
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2010
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crbenthamsciepub:10.2174/1874070701004010026 2023-09-05T13:22:23+02:00 Cryomicroscopic Investigations of Freezing Processes in Cell Suspensions Acharya, Tathagata Devireddy, Ram V. 2010 http://dx.doi.org/10.2174/1874070701004010026 https://openbiotechnologyjournal.com/contents/volumes/V4/TOBIOTJ-4-26/TOBIOTJ-4-26.pdf https://openbiotechnologyjournal.com/contents/volumes/V4/TOBIOTJ-4-26/TOBIOTJ-4-26.xml en eng Bentham Science Publishers Ltd. https://creativecommons.org/licenses/by/4.0/legalcode The Open Biotechnology Journal volume 4, issue 1, page 26-35 ISSN 1874-0707 Biomedical Engineering Biochemistry Bioengineering Biophysics Biotechnology journal-article 2010 crbenthamsciepub https://doi.org/10.2174/1874070701004010026 2023-08-11T15:33:38Z This study evaluates the freezing response of three different cell types, Pacific oyster embryos (~50 µm in diameter), Jurkat cells and HeLa cells (~12 to 15 µm’s in diameter), using cryomicroscopy. Freezing experiments were performed on oyster embryos at cooling rates of either 5 or 10 °C/min, while Jurkats were cooled at either 1 or 10 °C/min and HeLa cells were cooled at either 1, 15 or 20 °C/min, respectively. The experiments with oyster embryos were primarily designed to investigate the phenomena of intracellular ice formation (IIF) while the experiments for Jurkat and HeLa cells were designed to investigate both cellular dehydration and IIF during freezing. IIF was characterized by the abrupt black flashing during the cooling steps while the cellular dehydration experiments were characterized by the volumetric (projected area) shrinkage of the cells during the cooling steps. Mathematical models were fit to the cellular dehydration data to obtain the Jurkat and HeLa cell membrane permeability ( L pg ) at the reference temperature (273.15 K), the apparent activation energy of the cellular dehydration process ( E Lp ) or the temperature dependence of L pg . The temperature dependence of IIF in the oyster embryos, the Jurkat cells and the HeLa cells were also determined. Article in Journal/Newspaper Pacific oyster Bentham Science Publishers (via Crossref) Pacific The Open Biotechnology Journal 4 1 26 35 |
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Open Polar |
collection |
Bentham Science Publishers (via Crossref) |
op_collection_id |
crbenthamsciepub |
language |
English |
topic |
Biomedical Engineering Biochemistry Bioengineering Biophysics Biotechnology |
spellingShingle |
Biomedical Engineering Biochemistry Bioengineering Biophysics Biotechnology Acharya, Tathagata Devireddy, Ram V. Cryomicroscopic Investigations of Freezing Processes in Cell Suspensions |
topic_facet |
Biomedical Engineering Biochemistry Bioengineering Biophysics Biotechnology |
description |
This study evaluates the freezing response of three different cell types, Pacific oyster embryos (~50 µm in diameter), Jurkat cells and HeLa cells (~12 to 15 µm’s in diameter), using cryomicroscopy. Freezing experiments were performed on oyster embryos at cooling rates of either 5 or 10 °C/min, while Jurkats were cooled at either 1 or 10 °C/min and HeLa cells were cooled at either 1, 15 or 20 °C/min, respectively. The experiments with oyster embryos were primarily designed to investigate the phenomena of intracellular ice formation (IIF) while the experiments for Jurkat and HeLa cells were designed to investigate both cellular dehydration and IIF during freezing. IIF was characterized by the abrupt black flashing during the cooling steps while the cellular dehydration experiments were characterized by the volumetric (projected area) shrinkage of the cells during the cooling steps. Mathematical models were fit to the cellular dehydration data to obtain the Jurkat and HeLa cell membrane permeability ( L pg ) at the reference temperature (273.15 K), the apparent activation energy of the cellular dehydration process ( E Lp ) or the temperature dependence of L pg . The temperature dependence of IIF in the oyster embryos, the Jurkat cells and the HeLa cells were also determined. |
format |
Article in Journal/Newspaper |
author |
Acharya, Tathagata Devireddy, Ram V. |
author_facet |
Acharya, Tathagata Devireddy, Ram V. |
author_sort |
Acharya, Tathagata |
title |
Cryomicroscopic Investigations of Freezing Processes in Cell Suspensions |
title_short |
Cryomicroscopic Investigations of Freezing Processes in Cell Suspensions |
title_full |
Cryomicroscopic Investigations of Freezing Processes in Cell Suspensions |
title_fullStr |
Cryomicroscopic Investigations of Freezing Processes in Cell Suspensions |
title_full_unstemmed |
Cryomicroscopic Investigations of Freezing Processes in Cell Suspensions |
title_sort |
cryomicroscopic investigations of freezing processes in cell suspensions |
publisher |
Bentham Science Publishers Ltd. |
publishDate |
2010 |
url |
http://dx.doi.org/10.2174/1874070701004010026 https://openbiotechnologyjournal.com/contents/volumes/V4/TOBIOTJ-4-26/TOBIOTJ-4-26.pdf https://openbiotechnologyjournal.com/contents/volumes/V4/TOBIOTJ-4-26/TOBIOTJ-4-26.xml |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Pacific oyster |
genre_facet |
Pacific oyster |
op_source |
The Open Biotechnology Journal volume 4, issue 1, page 26-35 ISSN 1874-0707 |
op_rights |
https://creativecommons.org/licenses/by/4.0/legalcode |
op_doi |
https://doi.org/10.2174/1874070701004010026 |
container_title |
The Open Biotechnology Journal |
container_volume |
4 |
container_issue |
1 |
container_start_page |
26 |
op_container_end_page |
35 |
_version_ |
1776202903596302336 |